胚

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

It shows that the embryogenic tissues are composed of a group of small meristematic cells at one end (embryo-proper cells), which are at different phages of cell division and many long cells at the other end (embryo-suspensor cells).

研究表明，胚性愈伤组织是由不同发育阶段的早期胚组成的细胞团，早期胚主要由长的胚柄细胞及其顶端的胚头细胞组成，而后者又具不同的分裂相。

It was studied that the process of the somaticembryogenesis and analyzed that the problem existing in technique of in vitro morphogeny in Hevea to ground for that to probe into the method to improve the technology of tissue culture in Hevea with the techniques of transgene and to improve the ability of somatic embryogenesis and plant percentage of embryoid in Hevea.

本试验以巴西橡胶树，海垦2的花药为外植体，诱导体胚的发生，对体细胞胚发生方式及发育过程做了一系列的组织学观察，分析了橡胶树体细胞胚发生的各个过程以及畸形胚的形成原因，初步结果如下： 1 橡胶花药体细胞胚主要以间接方式发生。

With the prolonging of treatment with 6-DMAP,the developmental ability of parthenogenetic embryos decreased.

随着6-DMAP处理时间加长，孤雌胚的发育能力降低。6-DMAP处理1小时的桑葚胚／囊胚的发育率(72％)远远高于6-DMAP处理6小时的桑葚胚／囊胚的发育率(28％)。

Bioassay results revealed: compared with alcohol control, extracts at parameter 1, 4, 7 stimulated the absolute germination rate and potential, radicle growth, plumular axis growth, fresh weight, dry weight at different levels; extracts at parameter 2, 3 stimulated the all germination index except radicle growth and axis growth; extracts at parameter 6 stimulated all but radicle growth and fresh weight; extract at parameter 8 stimulated only the germination potential.

生物检测结果显示，与酒精对照相比：萃取参数1、4和7等条件苦楝叶提取物对杉木种子绝对发芽率、绝对发芽势、胚根长、胚轴长、鲜重和干重等发芽指标均表现出不同程度的促进作用，萃取参数2和3等条件苦楝叶提取对除胚根和胚轴外的各发芽指标起促进作用，萃取参数5条件苦楝叶提取物对除鲜重和干重外的各发芽指标起促进作用，萃取参数6条件苦楝叶提取物对除胚根和鲜重外的各发芽指标起促进作用，而萃取参数8条件苦楝叶提取物仅对绝对发芽势起促进作用。

In an attempt to promote the efficiency in citrus breeding and estimate the pos- sibility of embryo culture applied to citrus production,we carried out the culture technique in the hybrid embryos(Citrus sinensis Osbeek×Poncirus trifoliata Raf.).

为了提高柑桔杂交育种效率和探讨胚培养在柑桔生产上应用的可能性，我们研究了锦橙×枳(Poncirus trifoliata Raf。)的杂交种子胚培养技术。证明：怀特培养基+IAA(1ppm)+CH(600ppm)和 MT 培养基+GA(1ppm)能使第五个小胚萌发成苗；在 MT 培养基+2，4-D(0.7ppm)+BA(0.23ppm)上，有些胚先形成愈伤组织，然后从原有的胚中生根出苗。

The results of protein synthesis of NT embryos indicated that the protein synthesis pattern of NT embryos and fertilized eggs was similar. 19. 2 KD protein existed in 32-cell stage donor embryos disappeared in NT embryos at the pronuclear stage, however, this protein was reprogrammed to reappeared in 32-cell stage NT embryos.

七、核移植胚的蛋白合成分析结果表明，原核期核移植胚与原核期受精卵具有相同的合成模式。32-细胞期供体胚中的19.5KD蛋白在原核期重组胚中消失，而在32-细胞期重组胚中再度出现，发生了蛋白合成的重序过程。

In order to explain clearly the variation status of embryo from tetrapoid Robinia pseudoacacia and fully use the variation resource initiated in the sexual reproduction of TRP in the further improvement of TRP, the classification and vitalities of seed embryos from tetraploid Robinia pseudoacacia were studied. The main results are as follow. According to the cotyledon number, color and plumpness, the seed embryos of TRP could be divided into 5 types: YVT (yellow, very replete,2 cotyledons), YMT( yellow, more replete,3 cotyledons ), YRF ( yellow, replete,4 cotyledons ), GNT ( green, no-replete, 2 cotyledons ) and WNT ( white, no-replete, 2 cotyledons ).

为了阐明刺槐同源四倍体种子胚变异状况及充分利用刺槐同源四倍体有性过程创造的变异资源对四倍体刺槐进行遗传改良，研究了刺槐同源四倍体种子胚类型及其生活力特征，主要结论如下：刺槐同源四倍体种子胚按照子叶数量、颜色及饱满程度，可以划分出5个类型：黄二、黄三、黄四、绿二及白二，所有类型种子胚混合平均单胚重量接近二倍体的1/2。

This experiment object was 1-cell embryo of KUNMING mouse , ROS was added in different developmental phases through basal medium with CZB medium, cultured to morulae, early blastula, blastula and the hatching of blastula, observed the change of cell number and cleavage index of embryo.

本试验以昆明小鼠1-细胞期胚胎为实验对象，以CZB培养液为基础液在不同发育阶段添加外源性ROS，培养至桑椹胚、早期囊胚、囊胚、扩张囊胚阶段，分别观察胚胎的胚细胞数、分裂指数的变化。

Owing to placenta and milk transshipping effect, the content of Aroclor 1254 in fetal and infant vivo was very low, so there were not significantly pathological changes in sexual gland, the effect of Aroclor

2首次以排卵数、受精率、受精卵的第一次卵裂率、桑堪胚发育率、囊胚~孵化胚发育率、以及发育延迟胚和退化变性胚的比率，对个体发育的不同时期接触不同剂量 AroClor 1254对小鼠卵子发生、受精和附植前胚胎体内发育毒性进行系统定量。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失