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Embryo 1 to several by polyembryony.

1到数个多

Cleavage polyembryony occurs in some gymnosperms, e.g. in Pinus, but usually only one embryo develops to maturity.

裂生多现象通常发生在一些裸子植物中,例如松属,有时仅有一个可以发育成熟。

Seedlings under light absorbed more rutin than those in the dark at rutin concentrations of 60-80 μg/mL and absorbed less rurin at concentrations lower than 40 μg/mL or near 100 μg/mL. IAA (>0.1 μg/mL) treatment promoted rutin accumulation and inhibited elongation of shoot axis and primary roots.

当培养基中的芦丁浓度为60~80 μg/mL时,光照下的幼苗比暗处理的幼苗在轴中积累更多的芦丁;而芦丁浓度为40μg/mL以下和接近100μg/mL时幼苗在光照下累积的芦丁较暗处理的幼苗更少。0.1μg/mL以上的IAA促进芦丁的累积而进一步抑制幼苗轴和主根的伸长。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P<0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P<0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB,可明显提高双原核的形成率(38.9%vs 15.1%,P<0.05)和桑椹/囊发育率(40.7%vs 26.8%,P<0.05),但对其激活分裂率和总原核形成率无明显影响(P>0.05)。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件,以Hprt为阳性对照,用巢式RT-PCR对小鼠早期胎进行Xist基因的转录分析,结果发现,转录Sry基因的睾丸组织以及雄性胎,从受精卵发育到囊的过程中,基本上不转录Xist基因;不转录Sry基因的雌性卵母细胞和雌性胎,从出现原核开始,到发育至2-细胞期的过程中,Xist基因一直不转录,但是,从4-细胞期开始,一直到孵化前囊阶段,雌性胎都转录Xist基因。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞和二细胞间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胎中的表达。

When the porcine-porcine and porcine-mouse nuclear transfer embryos were activated,inthe former only one pronucleus was observed in most cases,but in the later two pronuclei werefound mostly.

猪-猪同种核移植胎和猪-小鼠异种移核胎激活后,异种重组多形成二原核(2PN),而同种重组则主要形成一原核。

Differentiation of the protoderm layer marked the beginning of the structural differentiation in globular stage.

球形时期表皮原层的分化标志着体细胞结构分化的开始。

The results showed that the coefficient of raicel decreased firstly in a short distance, and then kept stable form radicel base to radicel apical.

通过对根分支系数研究表明:从根基部到根尖,呈现出先递减,然后保持稳定的趋势,采用经验分布函数表示;同时,用四种分支类型已能描述玉米根分支特征,且它们的特征参数在一定范围内随机分布。

And Cucumis sativus L. were tested by bioassay-guided separation. The extracts from P. tomentosa flower with ethanol were extracted with petroleum ether, ethyl acetate and n-butanol. The inhibition rate of the ethyl acetate extract to growth of the 3 kinds of plant radicel were 99.89%, 63.53% and 32.74% respectively at 0.5 mg/mL concentration, and that of petroleum ether were 76.30%, 56.17% and 23.36%.

绒叶泡桐Paulownia tomentosa花乙醇提取物依次用石油醚、乙酸乙酯、正丁醇和水萃取后进行活性测定,结果表明,石油醚萃取物在浓度为0.5mg/mL时,对反枝苋、生菜和黄瓜种子根生长的抑制率分别为76.30%、56.17%和23.36%;乙酸乙酯萃取物在浓度为0.5mg/mL时,对反枝苋、生菜和黄瓜种子根生长的抑制率分别为99.89%、63.53%和32.74%。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。