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Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料,通过9~11 日龄非免疫鸡进行了病毒的实验室分离和传代,并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡矮小化、电镜特征等生物学特性鉴定,结果表明,4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞,标准阳性血清可特异性的抑制其凝集性,回归试验可复制出与自然发病相同的病例,病毒传代物有明显的致鸡矮小化作用,透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子,将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

These experiments demonstrated that hyperglycemic conditions in vitro can inhibit the growth and development of the embryo cells resulting in the down-regulated expression of igf-1, and IGF-1 could inhibit the apoptosis of blastocyst and be benefit for the growth of blastocyst.

因此,高血糖能抑制泡细胞的生长发育,导致igf-1的表达下调,而IGF-1能抑制泡细胞的凋亡,有利于泡细胞的生长发育国。

The frequency of somatic embryogenesis was less than 20%, which showed that somatic embryogenesis of Medicago truncatula is genotype-dependent.

性愈伤诱导体的发生率低于20%,表明品种的基因型在很大程度上影响Medcago truncatula体的发生率。

In this dissertation, serial researches have been carried out concerning the processing of wheat germ protein by micronization and alkali extraction, the preparation and the action mechanism of antioxidant hydrolysates from wheat germ proteins.

本论文以脱脂麦为原料,对麦蛋白在稀碱和微细化处理条件下的提取工艺进行研究,并对麦蛋白的酶解工艺、抗氧化活性及作用机理进行研究,主要研究内容及结果如下:1。

At the same time, there was remarkable difference in the calcium distribution on the wall of micropylar canal between self-pollinated and cross-pollinated ovules: abundant calcium precipitates were found on the wall of self-pollinated ovules, whereas none on cross-pollinated pollen.

同时,自交和异交授粉珠珠孔道壁钙信号表达也存在很大差异,自交授粉珠珠孔道壁有大量钙颗粒密集,而异交授粉珠珠孔道壁无钙沉淀积累。

Most of the a-sexual embryo sacs also parthenogenetically developed into asexual seeds,which is the same as that of their maternal parent.

此外,平邑甜茶也形成次生极核,并在卵分裂前,形成乳游离核;而卵的发育,无论受精与否都要经过3-5天休眠后分裂,并逐渐形成小球形、子叶

Seeds with copious starchy perisperm and a minute embryo embedded in small endosperm.

种子具大量的粉质外乳和一小嵌入在小的乳里。

Embryo spiral; perisperm separated into two parts by embryo, or perisperm absent.

螺旋形;外乳通过缺席胎儿或者外乳分成两个部分。

Seed vertical; embryo horseshoe-shaped; radicle inferior; perisperm copious.

种子垂直马蹄形;根下位。外乳丰富。

The results are as follows: The location of seedling in the polyembryonic rice IR36-Shuang could be differentiated into 2 types: the uncomplete twin seedlings and the complete twin seedlings.

结果表明,多苗突变株系&IR36-双&在双苗性状的表现形态上有其特异性,在同一纯合株系的群体内双苗的苗位有非完全双苗和完全双苗两种类型。

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