胚

It was confirmed that facultative apomixes is existed in coffee species based on emasculating treatment, cyto-genetic observe and SSR marker detection.

细胞遗传学观察发现咖啡四倍体类型材料(2n=4x=44)的多胚和多胚苗中含有天然的单倍体胚和单倍体小苗(2n=2X=22)；去雄处理后，可见子房膨大，其中5份材料获得了果实和种子，初步说明无融合生殖现象的存在；对小粒种品种间杂交F1代个体的SSR分析，发现多胚苗中存在与母本图谱相同且不携带父本特异带的后代以及携带双亲特异带的有性后代，表明子代有仅来自母本的无融合生殖个体分离出来。 3 建立了咖啡无融合生殖的分子鉴别方法。

The results indicated that the lignified seed shell had a negative impact on water permeability of seeds,but it was not the main reason for seed dormancy.

结果表明：(1)美国桂花种子木质化种壳对种子的吸水透性有一定的阻碍作用，但不是引起种子休眠的主要原因；(2)美国桂花种子的胚没有休眠，离体胚在光照培养7 d开始萌动；(3)胚乳被严密包裹的机械作用使胚无法获得萌发所必需的水分和氧气，这可能是导致种子休眠的最主要的原因。

At the same time,some ovule-specific cDNA clones in Orchid and Petunia flowers had been isolated and among them MADS Box genes may be responsible for the ovule differentiation and formation.

同时，已分别从蝶兰和矮牵牛的胚珠中分离出一批胚珠发育特异的基因，其中有关MADS Box 基因在胚珠形成和发育中的作用研究得比较清楚，基因转化工作证实胚珠的分化和形成受一类新的MADS Box基因控制。

With the increase of the 1254 dosages, in the further development process of 2-cell embryo, there still occurred the development relayed and degeneration, so that the development rate of morulae and blastocysts decreascd significantly.

在受精卵的进一步发育中，随著公鼠染毒剂量的增加，发育到桑椹胚和囊胚的比例显著降低，发育延迟胚及发育中退化变性胚比率显著升高。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

The galactosyl oligosaccharides of raffinose series are widely distributed in seeds of many species and are localized in tissues that remain viable after desiccation, including the embryo and aleurone layer of cereals, cotyledons and axis tissues of legumes and other dicots.

棉子糖半乳糖苷系列寡糖广泛分布在许多种植物种子中，并存在于干燥后仍能保持活力的组织内，如禾谷类种子的胚及糊粉层，豆类及其他双子叶植物的子叶和胚轴组织等。棉子糖半乳糖苷系列寡糖在禾谷类种子的非自溶性中央胚乳中不合成，但存在于蓖麻种子的自溶性胚乳细胞中。

A computer vision algorithm for identifying rice embryo and inspecting embryo ratio based on color analysis was developed.

通过对胚芽米的颜色特性和彩色图像的分析研究，首次提出以饱和度S作为特征参数进行胚芽和胚乳的识别，从而实现了对大米留胚率的自动检测。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

The changes in activity of glutamine synthetase and NADH-glutamate synthase in endosperms, coleoptiles, and roots were determined during the germination of rice Oryza sativa L.

测定了水稻种子不同萌发时期胚乳、胚芽鞘和幼根的谷氨酰胺合成酶和依赖于NADH的谷氨酸合酶活性变化。胚乳和胚芽鞘的GS活性在萌发过程中升高，幼根的GS活性则有所降低。

A lot of proembryos and heart-shaped embryos are abortive; however,the others may develop normally and grow into mature embryos.

胚在各个发育阶段尤其是球形胚和心形胚阶段，都可能败育，也可以正常发育成为分化完全的有效胚。2。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。