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The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的,可在体外发育至孵化囊阶段,我们应用一种简便、快速的异种重构的鉴定方法,即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构的核遗传物质来源于大熊猫供核体细胞(后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性)。

Functionally known genes were obtained after Blastx analysis, 237 of them were from the subtractive library of stage of proembryo mass and 279 from that of somatic embryo maturation.

成功构建了Y35体细胞团阶段和成熟阶段的差异表达基因文库,获得了868个差异表达的UniESTs,其中原团阶段400个,体细胞成熟阶段468个。

During embryogenesis, CaM mRNA was expressedstronger in the endosperm cells than in the proembryo cells at earlier stage but it wasreversed at embryo differentiation stage.

胎发育早期,钙调素mRNA在乳细胞中的表达比原中强,而后期则在分化中比乳细胞中强。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明,非性愈伤组织转入到MS + 4.0 mg · L-1 2,4-D + 0.5 mg · L-1 6-BA培养基培养后,愈伤组织中先出现一些性细胞;然后单个性细胞分裂形成一个由多细胞组成的原

The lung tissue for electron microscopy was cut into section 1mm thick sagittally.

将实验动物按年龄分为14日、16日、18日、20日、生后1日、1周、1个月、3个月、6个月、18个月共10组,每组6只。

Additionally, the water fraction from wood extract can inhibit the root growth on Pak-choi. At 1000 μg/mL, after 72 h, the length ratio of root to shoot was 0.69, and the dry weight of Pak-choi root was only the 20.6% of control. But the extracts of Sapindus mukorossi have no influence on the growth of Pak-choi with normal growth 7 days.

此外,木材抽出物之水分离部则具有抑制根生长的作用,经浓度1,000 μg/mL处理72 h之小白菜,根与茎长比值为0.69,且其根乾重仅为对照组的20.6%,但无患子抽出物对正常生长7天后之小白菜并无显著抑制生长作用。

The vascular bundle of stem is the type of double phloem. The anther is tetrasporangiate. Simultaneous cytokinesis in the microspore mother cell follows meriosis.

茎的维管束是双韧类型;雄蕊的花药四室,小孢子的形成为同时型胞质分裂,悬垂胎座,倒生珠,核型乳,果实成熟时仍处于球形阶段。

Nevertheless, human TGC-CM is not feasible in practical application. We hypothesized that xenogenic TGC-CM may exert similar influence on DPSCs.

我们培养发育期猪的第三磨牙牙,并收集获得牙条件培养基,同时培养人的牙并获得人牙条件培养基。

Embryoids occurred in all stages as zygotic embryo and were asynchronous. embryoids were white and opague.

分化经历与合子相似的发育过程,有球形期、心形期、鱼雷期和子叶期,体发生是不同步的,在同一块愈伤组织上有处于不同发育阶段的体

Fifty-five lines with different Wx genes from the DH population of Yangmai 10/Waxy C, and 12 waxy wheat varieties derived from the crosses or backcrosses of Yangmai 9 as no-waxy parent or recurrent parent with different waxy wheat varieties were used to study the effects of Wx genes on percentage of vitreous kernel. The grain protein content of 12 waxy wheat varieties with floury endosperm from different crosses was also tested.

为了明确Wx基因变异与小麦籽粒乳的关系,以玉米花粉诱导扬麦10号(角质乳品种,非糯小麦)与糯质供体Waxy C杂种F1产生的不同Wx基因变异类型的55个双单倍体系以及以扬麦9号与不同糯质供体亲本杂交、回交育成的12个糯小麦品系为材料,研究了Wx基因变异对籽粒乳质地的影响,同时测定了12个不同来源的粉质乳糯小麦籽粒蛋白质含量。

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