胎型

Primary culture of type II alveolar epithelial cells was performed using IgG panning technique. The experiment was divided into groups as VEGF, Dexamethasone, VEGF plus dexamethasone and a control, to determine total phospholipids, phosphatidylcholine, phosphatidyl glycerol and sphingomyelin.

方法孕19天Wistar大鼠剖腹取胎鼠，原代培养肺泡Ⅱ型细胞，分成VEGF组、地塞米松组、VEGF加地塞米松组和对照组进行试验，测定肺表面活性物质总磷脂、磷脂酰胆碱、磷脂酰甘油、鞘磷脂含量。

Objective To analyze the characteristics of hepatic metastasis of pure immature ovarian teratoma and explore proper diagnosis and treatment.

目的 总结单纯性卵巢未成熟型畸胎瘤肝脏四周复发的临床特征，探索其正确的诊断和治疗方案。

The plasmid of pEGFP-N1 was preserved in our laboratory.②The rat vibrissa follicles were dissected under a stereomicroscope. The dermal sheath was moved by incubated in dispase. The bulge regions of the hair follicles in anagen phase were carefully cut between the arrector pili muscle and the sebaceous gland, and then incubated with a mixture of trypsin and EDTA. The cell suspension was selected, and cultured in 10% fetal bovine serum DMEM/F12 FAD medium. After 7 days culture, HFSC was obtained by rapid adhering on collagen Ⅳ.

实验方法：大鼠在体视显微镜下分离出含真皮鞘的完整毛囊，dispase消化，将毛囊从真皮鞘中挤出，收集形态完好且处于生长期的毛囊，分别在毛球部上端、皮脂腺下端横切毛囊，取中间部分置于胰酶和EDTA中联合消化，向所得细胞悬液中添加含10%胎牛血清DMEM/F12完全FAD培养基，常规培养7 d后，采用IV型胶原快速贴壁法两次筛选以分离纯化大鼠毛囊干细胞。

Acrylic-oil, acrylic cool are commonly used such as acrylic paint, clear acrylic via ethhyl and butylacralate, in the resin introduction of hydroxyl and carboxylated to aminosulfonic cross-linkers gubernacular as a tree.

丙烯酸型平油，凡是拔取羟基丙烯酸酷如丙烯酸不纬乙酯和丙烯酸共聚，在树脂洋引进羟基和羧基，以氨基树胎作为接联剂。

After 6 to 7 days of culture, Eleven ES cell lines were selected and explanted. At last we obtained 4,3,4 and 3 ES cell lines separately. One of the B6C3F_1×B6D2F_1 ES cell lines was selected to future study its competent for germline transmission.We founded the ES cell line has normal karyotype on the chromosome analyse.In teratoma experiment by injected ESCs into C57 mices inguen, we gained teratoma contained cells come from all three embryonic germ layers.

选择一株具有高度杂合背景的B6C3F_1×B6D2F_1 ES细胞系进行核型分析、畸胎瘤实验后与ICR品系2n胚胎构建嵌合体并得到具有生殖系传递能力的嵌合体小鼠，证明此细胞系具有生殖系嵌合能力。

Refers to Down's syndrome (Down's syndrome, dementia-like伸舌) screening, that is, pumping blood or amniotic fluid do villus cells, and then do Karyotype analysis, such as the emergence of 21 - trisomy map, you can diagnose fetus for Down syndrome. 2, AFP Chinese name for the AFP, such as maternal and fetal amniotic fluid were increased AFP confirmed fetal neural tube defects, namely spina bifida, spinal Meningocele, no brains infants, microcephaly, hydrocephalus.

是指唐氏综合症的筛查，就是抽绒毛血或羊水做细胞培养，然后做染色体核型分析，如出现21-三体综合征图谱，即可诊断胎儿为唐氏综合征。2、AFP中文名称为甲胎蛋白，如母血及胎儿羊水AFP均增高，证实胎儿有神经管畸形，即脊柱裂、脊脑膜膨出、无脑儿、小头畸形、脑积水。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

A reactive nonstress test is reassuring. Patients with nonreassuring fetal heart rate testing should be delivered or further evaluated.

反应型无应激实验提示胎儿宫内情况好，胎心率检查可疑的患者应尽早分娩或进一步检查。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

RM has special morphological characteristics; it should be differentiated from gemis tocytic astrocytoma, atypical teratoid/rhabdoid tumor, and malignant melanocytoma.

RM有特殊的组织学特点，应注意与胖细胞型星形细胞瘤、非典型畸胎样瘤/横纹肌样瘤、恶性黑色素瘤等鉴别。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。