胎儿

Dimensiona ultrasonography data of 29 cases fetal anomaly between 24 and 40 weeks' gestation from Jan 2003 to Dec 2006. Results Among them, there were 11 cases of cheilognathus,7 cases of anencephalus, 4 cases of anomaly of extremities, 3 cases of omphalocele, 3 cases of lymphocyst at the neck and 1 case of amniotic band syndrome. The anomaly features are consistent with those of the born fetuses. Conclusion The three??

大部分解剖结构明显异常的畸形一般通过二维实时超声在22周左右即可做出诊断［1］，但胎儿体表结构有较复杂的曲面改变，二维超声可能漏诊、误诊胎儿体表畸形。20世纪80年代末应用于临床的三维超声成像，是检测胎儿发育状况的有效方法，大大提高了产前诊断的准确率，尤其在检测胎儿畸形方面具有独特优势［2］。

Paternally inherited fetal alleles were detected in 23 of 30 plasma samples. including 6/10 cases in early pregnancy, 8/10 cases in middle pregnancy and 9/10 cases in late pregnancy respectively.

结果 10例孕妇均足月分娩，男婴3例，女婴7例。10例孕妇血浆中均检出胎儿父源性等位基因，即胎儿DNA.30份孕期血浆标本中有23份检出胎儿DNA，其中孕早期标本6份（6/10）；孕中期标本8份（8/10）；孕晚期标本9份（9/10）。7份标本未见到胎儿DNA。

Objective To investigate clinical significance of color Doppler echocardiographic diagnosis in prenatal diagnosis of fetal congenital heart disease.Methods 520fetuses in24～40weeks were examined by color Doppler echocardiography on four-chamber views and subcostal views to determine CHD.

目的 探讨彩色普勒超声心动图对胎儿先天性心脏病产诊断的临床意义方法使用彩色多普勒超声诊断仪随机对520例胎龄24～40周的胎儿，在心脏超声四腔观基础上结合多切面观察胎儿心内结构，以确定胎儿有无先心病。

Results: The pollution of amnionic fluid, the incidence of Apgar≤7 after 1 minute and the perinatal mortality rate in the group of BPS≤5 were significantly higher than that in the group of BPS≥6 (P.05). To predict dysplasia with ultrasonic score of BPS, the positive predictive value, negative predictive value, specificity and sensitivity were 93.94%, 78.68%, 98.57%, 65.58% respectively.

结果 声振刺激后胎儿BPS超声评分≤5分组中胎儿羊水污染率、1min Apgar评分≤7分发生率、围生儿死亡率均明显高於BPS超声评分≥6分组(P.05)，声振刺激后胎儿BPS超声评分预测胎儿结局异常阳性值、阴性值、特异度、敏感度分别为93.94%、78.68%、98.57%、65.58%。

When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.

当用电融合法进行核移植时，成体耳部成纤维细胞的融合率（46.0％），卵裂率（53.9％）和囊胚发育率（10.9％）均显著低于胎儿成纤维细胞（64.5％，70.1％和21.6％），胎儿皮肤细胞（71.5％，70.8％和22.1％），以及卵巢颗粒细胞（88.2％，79.1％和25.5％）；另外三种细胞间的卵裂率，囊胚发育率无显著差异，但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞（88.2％vs 64.4％，71.5％，P＜0.05）。

Ultrasonographic scans, fetal genetic studies, and hormonal assays of amniotic fluid can diagnosis certain diseases, fetal sex differentiation disorders, fetal endocrinal disorders, and chromosome abnormality.

产前超声发现胎儿生殖器官异常可作为筛查指标之一，联合羊水中激素检查可早期诊断某些胎儿生殖器官异常、胎儿性分化异常、胎儿内分泌代谢异常、胎儿染色体异常相关疾病。

Abstract］ objective to discuss ultrasound diagnosis on fetal abnormal.methods we examined 1 231 example pregnant women carried on the prenatal with ultrasonography and the color ultrasonography.results picked out the fetal abnormal 9 examples,in which anencephaly were 2 examples,the hydrocephalus 3 examples,the meningocele 1 example,the endocardial cushion defects 1 example,the ectopiacordis accompanied grastroschisis 1 example,the cleftlip 1 example.all patients were confirmed through induced labor.conclusion fetal abnormal ultrasonography image is typical,the careful inspection correct analysis can discover promptly,ultrasonography is the first method to inspect the fetalabnormal,may achieve the prompt discovery,prompt termination of pregnancy item.

目的 探讨超声检查对胎儿畸形的诊断价值。方法对1231例孕妇进行产前超声检查。结果检出胎儿畸形9例，其中无脑畸形2例，脑积水3例，脑膜膨出1例，心内膜垫缺损1例，心脏外翻并腹壁大缺损1例，唇裂1例。所有患者均经引产后证实。结论胎儿畸形的声像图比较典型，细心检查正确分析能及时发现，超声是检查胎儿畸形的首选方法，可达到及时发现及时终止妊娠的目的。

Of the 19 cases,19 fetus were found,MRI confirmed 14 cases considered or suspected as fetal corpus callosum agenesis by US(8 cases are completed agenesis of corpus collosom-CACC,6 cases are partial agenesis of corpus collosom-PACC, and 5 cases had took on autopsy),however 3 cases are mild enlargement of lateral cerebral ventricle and 2 cases are leukodystrophy.In these cases,MRI confirmed a Dandy-Walker syndrome and a lipoma of corpus callosum suspected by US,detect a Dandy-Walker syndrome and a microcephalus made missed diagnoses by US.The fetal corpus callosum and additional cerebral anomalies were shown more clearly on MR T_2-weighted images.

胎儿胼胝体长度与额枕径的比值在24～36孕龄阶段相对较恒定。2、19例孕妇共检出胎儿19个，MRI证实超声疑诊的胎儿胼胝体发育不全14例(完全型胼胝体发育不全8例，部分型胼胝体发育不全6例，有胎儿尸检结果对照者5例)，3例为单纯侧脑室轻度扩张，2例考虑脑白质发育不良，合并Dandy—Walker综合征1例，胼胝体脂肪瘤1例，检出超声漏诊合并Dandy-Walker综合征1例及脑小畸形1例。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

It is concluded that the expression of hERMAP in fetal liver approximately consistent with haematopoiesis of fetal liver, and the expression of this gene in bone marrow aged 15-32th fetal weeks coincides with haematopoiesis of fetal bone marrow.

人类ERMAP基因在胎儿肝脏中的表达规律与胎儿肝脏造血过程基本一致，在15-32周胎儿骨髓中的表达规律与胎儿期骨髓造血活动基本吻合，推测该基因的功能可能与胎儿发育过程中红系细胞向肝脏和骨髓的迁移活动有关。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失