胎

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Determination of alpha-fetoprotein in amniotic fluid, fetal diagnosis may also open, such as neural tube defects.

测定羊水中甲胎蛋白，还可诊断胎儿开放性神经管畸形等。

Methods A retrospective analysis was conducted on 210 premature children from September 2005 to May 2008 with weight equal to or less than 2500g or a gestational age at birth of 36 weeks or less. All of the members were examined by indirect binocular ophthalmoscopy.

回顾性分析2005年9月至2008年5月来就诊的胎龄小于36周、体重低于2500g的210例早产儿的眼底筛查情况及高危因素。

Infant mortality has reduced continuously with signicant changes of distribution for material age and birth order and birth interval.

母亲分娩年龄、胎次和生育间隔的分布变动，显著地影响婴儿死亡率，其中生育间隔对嬰儿死亡的影响最强。

It seems so, Stephen said, when he wants to do for him, and for all other and singular uneared wombs, the holy office an ostler does for the stallion.

283"好像是的，"斯蒂芬说，为了这位贵族，并为所有其他特定的、未被耕耘过的处女的胎，他想尽尽马夫对种马所尽的那种神圣职责。

Otocyst cells ; stem cells ; cell proliferation ; differentiation ; fetal rats

听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠

Keywords otocyst cells; stem cells; cell proliferation; differentiation; fetal rats

听囊细胞；干细胞；细胞增殖；细胞分化；胎鼠

In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

CONCLUSION摘要: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

结论摘要：胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

Month culture. CONCLUSION: In natural culture condition in vitro, otocyst cells of fetal rats can differentiate or proliferate to immature hair cells.

胎鼠听囊细胞在体外自然培养条件下可以分化或者增殖为未成熟毛细胞。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。