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All single step method and CTAB method did not work well when banana total RNA was isolated, unless modified single step method was used, in which, polysaccharide was removed by aether extraction prior to RNA precipitation.

对于富含多糖的香蕉果肉,一步法和CTAB异硫氰酸法的效果均不理想;改良后的一步法中增加了乙醚去除多糖并获得了较好的效果。

The total RNA were extracted from CDV infected DK cells or the tissues of infected animals by single step method of acid guanidinium thiocyanate-phenol-chloroform extraction and was used for reverse transcription.

用异硫氰酸-酚-仿一步抽提法提取细胞总RNA进行反转录,再以此产物为模板进行PCR扩增,筛选出最佳扩增条件。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA,在含有甲醛的琼脂糖凝胶电泳后,转印到硝酸纤维膜上。

Methods The template DNA was extract from the human osteosarcoma cells line U-2OS by the single- step isolation method with isothiocyanic acid guanidine, the cDNA coding for the mature fragment of BMP-4 was amplified by the reverse transcription- polymerase chain reaction.

方法采用异硫氰酸一步法从人骨肉瘤细胞株U-2OS中提取总RNA,通过逆转录-聚合酶链反应扩增为BMP-4成熟区cDNA基因片段。

In addition, the subjects who took guanfacine had significantly more somnolence, lightheadedness, and dry mouth than those who took placebo.

另外,服用氯苯乙的患者比服用安慰剂的患者有更明显的嗜睡、头昏、口干等症状。

Agmatine is the precursor of spermine and spermidine; however, neither of the two polyamines influenced morphine analgesia.

丁胺是精胺及亚精胺的合成前体。精胺和亚精胺均不影响吗啡的镇痛作用。

B:I'll give you some sulfaguanidine tables.

我给您开些磺胺片。

Samples of animal manure were collected in Jiangsu Province in 2005 to 2006, the residues of sulfagidine, sulfanilamide, sulfadiazine, sulfamerazine(SM1), sulfadimidin(SM2), sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole were determined by HPLC with fluorimetric detector after pre-column derivatization.

2005—2006年采集了江苏省各市不同种类的集约化畜禽养殖场共178个畜禽粪便样品,用柱前衍生法高效液相色谱荧光检测器,检测了样品中8种磺胺类药物(磺胺、磺胺、磺胺嘧啶、磺胺甲嘧啶、磺胺二甲嘧啶、磺胺间甲氧嘧啶、磺胺氯哒嗪、磺胺甲恶唑)残留情况。

In addition, the preparation conditions of butylbiguanide sulphate were studied by orthogonal test.

此外,用正交设计对正丁基双硫酸盐的合成反应条件进行了研究。

Nine kinds of compounds are synthesized with NTO〓, PA〓, TNR〓'used as acid radical, and CHZ, SCZ·HCl, AG·H〓CP〓 served as the materials. Among them eight kinds of compounds have been never reported in the literature and one has been prepared at home for the first time. The structures of these compounds are characterized by using the element analysis and the FT-IR measurements.

以3-硝基-1,2,4-三唑-5-酮离子、2,4,6-三硝基苯酚离子、2,4,6-三硝基间苯二酚离子为酸根,以碳酰肼、盐酸氨基脲、氨基重碳酸盐为原料合成富氮化合物,得到了8种未见文献报道的新富氮化合物,另有1种是国内首次合成的新型富氮化合物。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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