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胆红素

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Methods: Seventy-two confirmed BMJ neonates were observed and analyzed. The total bilirubin of seventy cases was greater than 221 μmol/L. The 22 cases whose total bilirubin was greater than 342 μmol/L were treated, on the basis of phototherapy, with Yinzhihuang, smecta and combined traditional Chinese and Western medicine to retreat yellowy, and breast-feed was suspended for 48-72 hours.

72例确诊为母乳性黄疸新生儿血清总胆红素>221μmol/L者70例,其中总胆红素>342μmol/L者22例,在光疗及茵栀黄、思密达中西医结合退黄治疗的基础上,暂停母乳48~72h。

Methods The levels of serum TBA, total bilirubin, direct bilirubin, alanine aminotransferase, and glutamic oxaloacetic transaminase of 215 normal pregnant women and 136 non-pregnant women were determined by Olympus Au-640 whole autochemical analyzer.

采用日产OlympusAu-640自动生化分析仪检测215例正常孕妇不同妊娠阶段和136例未孕妇女血清总胆汁酸含量,并同时检测总胆红素,直接胆红素、谷丙转氨酶、谷草转氨酶。

CSF UCB is a reliable indicator in diagnosing bilirubin encephalopathy. The ratio of B/A is a more sensitive indicator of bilirubinic toxicity than serum UCB.

CSF中UCB可作为胆红素脑病诊断可靠指标;B/A作为胆红素毒性指标较血UCB更有意义。

Infusion of theChinese prescription "Ying-chen dandao tang" in 28 cholelithiatic patients who had had cholecystectomy choledochotomy with a "T" tube drainage (15 cases in experimental group and 13 cases in control group).

发现,在胆酸丢失、胆汁成石指数增高、胆汁成石性增高的情况下,应用该制剂时,胆汁成石指数无明显变化;并且胆汁中总胆红素、非结合胆红素浓度及胆汁UCB/T Bil%值等均较注药前明显降低。

It was found that after the intravenous injection of bilirubin into a rat the concentration of both bilirubin and glucuronic acid in the bile rose.

在给大鼠静脉注射胆红素之后发现在胆汁中的胆红素和葡萄糖醛酸浓度升高。

Although cholestasis of sepsis is often associated with gram-nagetive bacterial infection,it also occurs with a diverse array of other organisms,including Gram-positive bacteria,rickettsial,parasitic and fungal infections.in one study of bacteraemic patients with positive blood cultures only 34% had bilirubin levels greater than or equal to 2.0mg/dl.moreover,in clinical practice the frequency and extent of liver enzyme abnormalities in bacteraemic patients may be far greater than the occurrence of hyperbilirubinaemia.cholestasis appears to be a marker for the severity of sepsis,and prolonged and unresolved cholestasis carries a high mortality.

虽然败血症(sepsis引发的胆汁郁积往往与革兰氏阴性菌(gram-negative bacterial,原文negative瓶错了)感染,它也可能伴随各种各样其他的有机体,包括革兰氏阳性菌(Gram-positive bacteria),立克次体属微生物,寄生虫和真菌感染。一个对菌血症(bacteremic,原文中拼错了)的阳性患者的血液培养分析研究发现其中只有34%的胆红素水平大于或等于2.0mg/dl。而且,在临床实践中的血症患者肝酶异常的发生频率和严重程度可能远远大于高胆红素血症(hyperbilirubinemia,原文中拼错了)。胆汁郁积似乎是一个患有严重性败血症的标志,而且长期的和没有根治的胆汁郁积有很高的死亡率。

Methods Serum total bilirubin was determined by vanadic acid oxidation method, improved J - G method and Bilirubin Oxidase method.

目的:对钒酸氧化法、改良J—G法、胆红素氧化酶法进行方法学比较,选择胆红素测定的最好方法。

There were not obviously interference to vanadic acid oxidation method when TG≤8.0 mmol/L, HB≤6.0 mmol/L.

钒酸氧化法测定胆红素准确、灵敏、稳定,线形范围宽,是测定胆红素的理想方法。

Results The within-run and between-run coefficient of variation of total bilirubin by vanadic acid oxidation assay were 1.58% and 2.94%.The within-run CV of direct bilirubin were 1.23% and 2.7%. The average rate of recovery of TBil and DBil were 101.1% and 100.1%.

结果 钒酸氧化法测定血清总胆红素批内变异系数为1.58%,批间CV为2.94%;血清直接胆红素批内CV为1.23%,批间CV为2.7%;平均回收率TBil为101.1%,DBil为100.1%。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T/BOX切下,插入到表达质粒pET-3a的T7启动子下游,构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a/BOX。

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