胆汁酸

Objective To explore the effect of high serum concentration of bile acids on energy metabolism of liver.Methods Bile acids were continously infused into the Wistar rats to simulate the serum concentration change of patients with obsturctive jaundice.Serum bile acids concentration,arterial blood ketone body ratio and energy charge of liver were determined.Results Aterial blood ketone body ratio and energy charge of liver decreased with the increase of serum bile acids concentration.Conclusion High serum concentration of bile acids plays an important role in hepatic fumction injury caused by biliary obstruction.

目的 探讨高胆汁酸血症单一因素对肝脏能量代谢的影响方法经Wistar大鼠颈内静脉持续给予外源性胆汁酸，升高其外周血清中胆汁酸浓度，模拟阻塞性黄疸患者外周血清中胆汁酸的变化规律，观察腹腔动脉血酮体比值及肝组织能荷变化，时间一周结果随外周血清中胆汁酸浓度的升高，腹腔动脉血酮体比值、肝组织能荷均呈进行性下降结论高胆汁酸血症时肝脏能量代谢受损，表明在阻塞性黄疸患者肝功能损害中高胆汁酸血症起了重要作用

Methods Bile acids were continously infused into the Wistar rats to simulate the serum concentration change of patients with obsturctive jaundice.Serum bile acids concentration,arterial blood ketone body ratio and energy charge of liver were determined.

经Wistar大鼠颈内静脉持续给予外源性胆汁酸，升高其外周血清中胆汁酸浓度，模拟阻塞性黄疸患者外周血清中胆汁酸的变化规律，观察腹腔动脉血酮体比值及肝组织能荷变化，时间一周。

Glycocholic acid is a kind of glycol-conjugated bile acid which exists in many animals'bile. It is one of main component of bile acid and was synthesized from cholalic acid and glycine in liver.

甘氨胆酸是胆汁酸的主要成分之一，属于结合型胆汁酸，在肝脏中由胆酸和甘氨酸结合而成。

The mean conˉcentrationsof the total bile acid in the gallbladders were respectively77.53±11.93,51.81±8.12and53.27±7.04in group DM,CH and DMCH,but it was79.28±18.28in group N.Combined bile acids（both glycocholic acid and taurocholic acid）were apparently lessened and the ratio of cholic acid and chenodeoxycholic acid was rised in group DM,CH and DMCH,the concentrationsof cholesterol were respectively5.31±0.81,5.69±2.20and5.12±1.47,but0.86±0.20,in the group N;those of phospholipd were respectively0.35±0.16,0.52±0.18and1.38±0.34,but0.59±0.28,group N.

胆囊胆汁成分分析中，DM组、CH组和DMCH组总胆汁酸分别为77.53±11.93、51.81±8.12和53.27±7.04，N组为79.28±18.28；与N组比较结合胆汁酸减少，其中甘氨胆汁酸盐与牛磺胆汁酸盐均减少；鹅去氧胆酸减少，胆酸/鹅去氧胆酸比值升高；胆汁胆固醇分别为5.31±0.81、5.69.20和5.12±1.47，N组为0.86±0.20；磷脂分别为0.35±0.16、0.52±0.18和1.38±0.34。

Stones were seen in the gallbladder of12/12cases in either group DM and CH,and11/12in the group DMCH.The mean conˉcentrationsof the total bile acid in the gallbladders were respectively77.53±11.93,51.81±8.12and53.27±7.04in group DM,CH and DMCH,but it was79.28±18.28in group N.Combined bile acids（both glycocholic acid and taurocholic acid）were apparently lessened and the ratio of cholic acid and chenodeoxycholic acid was rised in group DM,CH and DMCH,the concentrationsof cholesterol were respectively5.31±0.81,5.69±2.20and5.12±1.47,but0.86±0.20,in the group N;those of phospholipd were respectively0.35±0.16,0.52±0.18and1.38±0.34,but0.59±0.28,group N.

胆囊胆汁成分分析中，DM组、CH组和DMCH组总胆汁酸分别为77.53±11.93、51.81±8.12和53.27±7.04，N组为79.28±18.28；与N组比较结合胆汁酸减少，其中甘氨胆汁酸盐与牛磺胆汁酸盐均减少；鹅去氧胆酸减少，胆酸/鹅去氧胆酸比值升高；胆汁胆固醇分别为5.31±0.81、5.69.20和5.12±1.47，N组为0.86±0.20；磷脂分别为0.35±0.16、0.52±0.18和1.38±0.34。

It was observed that, at the doses of 50 mg/kg and 100 mg/kg, geniposide could significantly increase the biliary secretion after intraduodenal administration, while crocins showed no effect.

结果显示西红花苷50和100 mg/kg剂量均不增加大鼠的胆汁流量，而京尼平苷50和100 mg/kg剂量均可显著增加大鼠胆汁流量，降低胆汁内胆固醇含量，增加胆汁内HCO－3浓度，但对胆汁酸，胆红素，〔Ca2＋〕含量无显著影响。

The oversaturation of cholesterol in bile results in the formation of cholesterol stones. The liver is the organ which synthesizes cholesterol and secretes bile. Liver lipid metabolism research helps to explain the mechanism of cholelithiasis. Utilizing biochemical and biomolecular technology, we have done experimental work as follows: Analyse the ingredient changes of blood and bile samples from patients.

肝脏是合成胆固醇、分泌胆汁酸的器官，对肝脏胆固醇代谢的研究有助于阐明胆石症形成的原因，本文用生物化学和分子生物学的方法，分析了病人血液、胆汁中各种脂类成份的变化，以及肝脏胆固醇合成限速酶3-羟-3-甲戊二酸辅酶A还原酶（HMG-CoA Reductase）、胆汁酸合成限速酶7α-羟化酶（7α-hydrolase）的基因表达。

Approximately some 1/4 unio primary bile acid in the jejunum,the ileum and the colon upside ,with the effect of enzyme's catalysis,produces the deoxycholic acid,the lithocholic acid ,the bear deoxycholic acid and etc.

大约有1/4的结合型初级胆汁酸在空肠、回肠及结肠上段，在细菌的酶的催化下，进一步生成去氧胆酸、石胆酸、熊去氧胆酸等次级胆汁酸。C·胆汁酸的合成：肝细胞合成的胆汁酸与甘氨酸或牛磺酸结合，形成结合型胆汁酸，分泌入胆道。

When the swine's bile acid, made from the waste liquid of swine's bile after bilirubin is extracted, is used as an antitussive medicine to treat the mice's cough induced by SO2 the following result is recorded: The swine's bile acid is effective in prolongating the cough latency and decreasing the cough frequency per unit time.

本文利用提取胆红素后的猪胆汁废液所制备的猪胆汁酸，作对SO_2诱发咳嗽的正常NIH系小白鼠镇咳效果观察。结果表明；猪胆汁中的胆汁酸对SO_2诱发咳嗽的小白鼠具有延长诱发咳嗽潜伏期和减少单位时间内咳嗽次数的效果。

Biles from an animal model of incomplete stenosis of common bile duct in guinea pigs and from patients with and without gallstone were examined for the total protein concentration, protein fractionations by SDS-PAGE electrophoresis, endogenous and exogenous .beta.-glucuronidase activity, bilirubin fractionations by HPLC, ionized calcium activity, pH, cholesterol and bile salts concentrations.

我们利用天竺鼠总胆管不完全结扎的动物模式之胆汁与患有胆结石病人之胆汁，进行下列测定：总蛋白质浓度、SDS-PAGE蛋白质电泳分析、内源性与外源性葡萄糖醛酸酵素活性、胆红素高效能液态层析仪分型浓度、游离钙活性、pH值、胆固醇浓度与胆汁酸浓度。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。