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Methods Detecting bile acid metabolites with high performance liquid chromatography-mass spectrometry to serum samples of five patients of consuming amanita verna.

采用高效液相色谱联合质谱技术检测了5例不同预后的白毒伞中毒患者的多种胆汁酸代谢产物。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定;用酶法测定了alt、ast与胆汁酸并计算ast/alt值;用peariman和lee改良法测定血清胆红素;用溴甲酚氯比色法测定血清白蛋白;用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原;并测定了凝血酶原时间与血小板计数等相关指标。

The staining of F-actin in the ischemia reperfusion group was decreased significantly (P.05) compared with the normal control group. There was a marked loss of canalicular microvilli and distension of canalicular lumen in the ischemia reperfusion group. The staining of F-actin in the LA group was significantly increased than those in the I/R group (P.05). Conclusions: Ischemia reperfusion induces an disruption of bile canalicular F-actin microfilaments and a loss of microvilli. Alpha-lipoic acid can protect bile canalicular F-actin microfilaments against IRI.

肝脏I/R可造成胆小管F-actin微丝破坏、微绒毛丧失,导致胆小管收缩减弱,胆汁排泄功能受损,这可能是大鼠肝脏I/ R后缺血型胆道病变发生的主要机制;α-硫辛酸通过清除氧自由基,降低脂质过氧化反应,调节细胞内氧化剂-抗氧化剂的含量,保护胆小管F-actin微丝结构免遭缺血再灌注损伤破坏,进而预防和治疗缺血型胆道病变。

Results The pathogenesis of XGC was due to bile exudation to interstitial of cyst wall from reptured Rokilansky-Aschoff sinus or mucosa ulcer.

结果 XGC发病机理为胆汁从破裂的Rokitansky-Aschoff窦或粘膜溃疡处渗至间质,产生炎症反应。

Maternal plasma and umbilical cord vein concentration of LPO and the amount of RBC-SOD were measured by thiobarbituric acid fluorimetric determination and catechol self-oxidation determination respectively.

目的 探讨过氧化脂质和红细胞超氧化物歧化酶活性在妊娠肝内胆汁瘀积症病理生理变化中的作用。

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