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Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示,各组织结构正常,细胞形态清晰可见;免疫组织化学SABC染色显示,在皱体部固有层底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒;western Blot 实验发现,在和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在中表达量显著高于小肠中的表达;110KDa的短型瘦素受体蛋白,在小肠和皱中表达量接近。98KDa短型受体蛋白在和小肠表达均较弱。

Fifteen patients underwent operation, including gastrostomy (6 cases), gastrotomy (4 cases), partial gastric fundus resection (2 cases), others operations (2 case), and laparotomy exploration (1 case); among them four patient died.

手术治疗15例,包括切开减压、造口术(6例),切开减压后再缝合创口(3例),切开减压、造口、空肠造口术(1例),底部分切除术(2例),十二指肠空肠吻合术(1例),空肠造口术(1例),开腹探查术未切开壁(1例),死亡4例(26.7%)。

Why the Friends of the stomach of sugar,"not as" stomach movements,"Reserve","grinding","send" three functions, if the row to send dysfunction, you can not complete the normal gastric emptying function and lead to retention, that is, stomach pump failure, also known as gastric "not as" clinically referred to as the stomach ...

糖友的缘何"不作为"的运动有"储"、"磨"、"送"三种功能,若其排送功能不良,则不能完成正常的排空作用而导致潴留,这就是泵衰竭,也可称为"不作为",临床上称为。。。

Results 25 protuberances from outside organ (spleen, n=19; aorta, n=4; left hepatic lobe, n=1 and cyst of pancreatic tail, n=1) and 92 SMTs were detected by EUS including 63 myogenic ones (esophageal, n=34; gastric, n=29), 9 etopic pancreases in stomach, 8 esophgeal cysts, 3 varices in gastric fundus, 3 gastric lymphomas, 2 gastric lipomas, 2 Menetrier ' s disorders, 1 esophageal lipoma and varix each.

结果发现外压性改变25例(其中脾脏19例,主动脉弓4例,肝左叶、胰尾囊肿各1例);粘膜下肿瘤92例,包括肌源性肿瘤63例(食管34例、29例),异位胰腺9例,食管囊肿8例,底静脉瘤3例,淋巴瘤3例,脂肪瘤2例,巨大粘膜肥厚症2例,食管脂肪瘤、食管静脉瘤各1例。

P 0.05. Total ruminal bacteria, proteolytic and amylolytic bacteria, and protozoa in rumen enumerated by culture method all increased (P 0.05) in the Trial period. Real-time PCR analysis was used to compare Pre-trial and Trial populations of cellulolytic species, including Butyrivibrio fibrisolvens, Ruminococcus flavefaciens and Ruminococcus albus, which were increased by 7.0, 4.5 and 9.6%(P 0.05), respectively. Two starch-fermenting bacteria species, Streptococcus bovis and Ruminobacter amylophilus, were increased by 25.4% and 14.6%(P 0.05), respectively.

3.02,P 0.05。传统培养法结果表明,与对照期相比,BSN2处理期奶牛瘤中瘤细菌、蛋白分解菌、淀粉分解菌和原虫数量显著增加;实时定量PCR结果表明,试验期奶牛瘤纤维分解菌,包括溶纤维丁酸弧菌、黄色瘤球菌和白色瘤球菌数量分别提高6.97%、4.51%和9.6%(P 0.05),两种淀粉分解菌,牛链球菌和嗜淀粉瘤杆菌分别提高25.4%和14.6%(P 0.05),乳酸利用菌埃氏巨球型菌也提高8.85%(P 0.05)。

Results ①The most common lesions were the extragastric compressions caused by spleen, which accounted for 54.74%, followed by the enlarged gallblodder, hepatic cyst and carcinoma in the left lobe, left kidney, pancreatic cystic adenoma and pancreatic cyst.②The diagnostic accurancy of EUS was 100%, which was higher than that of USP.③Most of the lesions, benign or malignant, had normal structure of the gastric wall.④Splenomegaly only accounted for 38.46% in the lesions caused by the compression of spleen, which were slightly to moderately enlarged.⑤The location of the lesion was usually related to the organ which caused the extragastric compression.

结果 ①外压性隆起中脾脏压迫最多见,占54.74%,其余为肿大胆囊、肝右叶囊肿、肝左叶癌、左肾、胰腺囊腺瘤和胰腺囊肿;②EUS对外压性隆起的诊断正确率达100%,其对外压灶的完全显示率高于微超声探头;③良性外压灶及多数恶性肿瘤外压灶其所压壁结构均正常;④脾脏压者中脾肿大仅占38.46%,且均为轻、中度脾肿大;⑤隆起部位与外压灶的种类相关联。

Following studies had been performed:(1)repetitive WLT and real-time ultrasonography had been performed to 18 HS and 40 FD toassess the reproducibility and influencing factors.(2) correlations of WLT with symptomscore and electrogastrography had been analysed.(3) differences ofWLT between HS and FD had been compared.(4) methophenolane andimmunohistochemistry stain of gastric fundus mucosa biopsy from 40 FD patients hadbeen performed to analyse the correlations of symptom score and WLT with count andactivity of mast cells, 5-HT level, and H.Pylori infectious status.(5) the effect of oralsumatriptan to proximal stomach function in 10 HS and 10 FD had been assessed, so as toestablish the possible regulating mechanism of fundic relaxation, explore the therapeuticpotential and feasibility of sumatriptan on FD.Results:I. Evaluation of WLT and its application in FD management.

我们主要进行以下几个方面的研究:(1)对18名健康人及40名FD患者重复进行水负荷试验并结合B超声对近端的实时监测,评价水负荷试验结果的可靠性并分析其影响因素;(2)观察水负荷试验结果与症状积分及体表电结果的相关性;(3)比较FD患者和健康成人水负荷试验结果的差别;(4)对40名FD患者底粘膜活检组织进行免疫组化染色和甲苯胺蓝染色,观察FD患者的症状及水负荷试验结果与底粘膜肥大细胞、5-HT表达情况及H.pylori感染情况的相关性:(5)观察口服舒马曲坦对10名健康人及10名FD患者近端功能的影响,并由此推测底舒张运动调节的生理机制,探讨口服舒马曲坦治疗FD的可行性。

Mobilization of the stomach for use as the conduit is performed, with dissection of the celiac and left gastric lymph nodes, diision of the left gastric artery, and preseration of the gastroepiploic and right gastric arteries.

游离并作管状时,需解剖腹腔和左淋巴结,分离左动脉,并保护网膜和右动脉。

Motilin and gastrin induced contraction of isolated gastric smooth muscle cells in a dose-dependent manner.(2) Motilin- and gastrin-induced antral smooth muscle cell contraction was inhibited by antibodies against Gαi-3. Motilin and gastrin caused an increase of Guanosine-5′-(3-[35S]thio)triphosphate([35S]GTPγS) binding of Gαi-3.(3) Contraction of antral muscle cells induced by motilin and gastrin was inhibited by the phospholipase C inhibitor U-73122 and the IP3 receptor antagonist heparin.

结果显示:(1)动素和泌素对窦平滑肌细胞均有收缩作用;(2) Gαi-3抗体可抑制动素和泌素加强窦平滑肌细胞的收缩,动素、泌素明显增加Gαi-3抗体与[35S]GTPγS的结合;(3)磷脂酶C抑制剂U-73122、三磷酸肌醇受体拮抗剂肝素可抑制动素和泌素引起的窦平滑肌细胞的收缩。

Methods insert the stomach tube nasal cavity, with the help of intubating ferceps, take the stomach tube out of mouth cavity from the posterior wall of dysphagia, insert trachea catheter (cid 7.0~7.5) to esophagus, under the help of trachea catheter, insert the stomach tube to esophagus then take the trachea catheter out of the esophagus and vertical, cutting off, in the course of into esophagus.

经鼻腔插入管,用插管钳将管从咽后壁前取出口腔外,用id 7.0~7.5的气管导管插入食管,在气管导管诱导下将管经气管导管插入食管,然后将管向食管内送进,同时将气管导管退出,将管置入相当合适的深度后,固定管。

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