胃

A 46-year-old woman, known to have radiation enteritis with chronic diarrhea and associated malabsorption syndrome, presented to the outpatient department complaining of severe tingling of recent onset in her hands and feet. The patient had a past history of hypocalcemic tetany 18months previously, but serum calcium had since remained normal on therapy with 1α-hydroxycholecalciferol, 0.75μg daily, plus oral calcium supplements.

您好。。。想请问医生，我在以前就有胃痛的症状〈大约3年了〉，曾经有中医师跟我说我这是季节性胃炎，后来换了工作之后，胃痛的机率比较少了，我就没那么在意这件事了，但是，在这近几个月来，胃痛的症状又开始出现，但是，说痛它也没有那么痛，就好象是闷闷的痛，而且不只是闷痛，有时还觉得人有点晕晕的，因此，想请问医生这是什么症状呢？

Results: DWN could lessen gastric mucous injury, inhibit ulcerous formation and improve pathological changes of damaged gastric mucosa induced by alcohol.

结果：胆胃宁能减轻乙醇所致胃黏膜损伤，抑制溃疡形成，改善胃黏膜组织学损害。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40％ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的： 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法：用酒精灌胃法制备大鼠酒精性肝病模型，将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只)，连续灌胃8周后病理切片观察细胞凋亡程度，干化学法测定ALT、AST的含量变化，用PCR法测定细胞色素P450 2E1的表达变化。

Methods The mice were divided into three groups. The alimentotherapy group was received honey milk tea, the medicine group received ranitidine, and the blank control group received distilled water. All the mice were treated in 8 successive days.

将小鼠分为三组，食疗组用蜂蜜奶茶25ml/kgBW灌胃8d，并用蜂蜜奶茶代替蒸馏水饲养；药物组用雷尼替丁42ml/kgBW灌胃8d，对照组用蒸馏水灌胃8d，后两组均用蒸馏水饲养，三组均食用相同的固体饲料。

Methods: Diabetes gastroparesis model rats were built by means of intraperitoneal injection 5% alloxanate aqu and Prepared Radix Rehmanniae once. Observed the effect of JG on rats' stomach power, serum glucose, serum motilin, cholecystokinin.

采用一次性腹腔注射5%四氧嘧啶水溶液加熟地复制糖尿病胃轻瘫模型，观察加味柴平颗粒剂对大鼠胃动力、血糖、血清胃动素、胆囊收缩素的影响。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Results: Among 55 cases of fetus digestive malformation, 3 cases without stomach bubble, 2 cases with small stomach bubble, 7 cases with double bubble, 1 case with strong echo around liver and spleen, 1 case with cyst in liver, 2 cases with solid mass in liver, 1 case with large liver, 2 cases with small intestinal extension, 11 cases with colon extension, 1 case with aproctia, 18 cases with cyst in abdominal cavity, 6 cases with strong echo in abdominal cavity.

结果：55例患者中，胎儿腹腔内未见胃泡3例，小胃泡2例，胃泡呈&双泡征&7例，肝脏及脾脏近包膜处强回声1例，肝脏囊性占位1例，肝内实性占位2例，肝脏大1例，小肠扩张2例，结肠扩张11例，先天性无肛门1例，腹腔内囊性占位18例，腹腔内强回声6例。

Agreat deal of cottons,plastic bags,ropes and bricks eaten by this goat were deposited in stomach and resulted in atonia proventriculorum which affected its natural emptying inclusion.

由于饲养管理粗放，波尔山羊发生矿物质营养代谢障碍，产生异食癖，吃进大量棉花、塑料袋和绳套砖块等，在胃内贮积，导致前胃弛缓，影响胃的排空。

The method makes model of small rat gastric ulcer, go alone with the home stomach of fill of colza leach fluid gives drug, observation test group and contrast the cankerous index of mucous membrane of group of small rat stomach, discuss its to advance a law to observe the home goes alone with charcoal end to protective action; of the stomach the influence that colza water steep moves to small intestine passes experiment leaving put oneself in another's position to observe its the action; of flowing to small rat small intestine flesh observes with heating up board law the home goes alone colza water.

方法制作小鼠胃溃疡模型，以家独行菜子水浸液灌胃给药，观察实验组和对照组小鼠胃黏膜的溃疡指数，探索其对胃的保护功能；以炭末推进法观察家独行菜子水浸液对小肠运动的影响并通过离体实验观察其对小鼠小肠平滑肌的功能；以热板法观察家独行菜子水浸液对实验动物的镇痛功能。

Via the experimental study of HWJND to SUs model in rat, we discovered that HWJND can reduce the excretive capacity of gastric juice and cankerous index of gastric mucosal, increase the content of GMBF, PGE2 in gastric mucosal, depress the content of NO and iNOSmRNA in gastric mucosal etc at a certain extent according to 15、 20 or 25g medicinal materials/kg avoirdupois. The results have statistic significance (p. 05-0. 01).It clues to that HWJND

和胃降逆方能通过多种途径多种机制表现出对SU模型大鼠胃粘膜的防护作用，同时体现了中药多成分、多靶点、多作用环节的特点，而和胃降逆方具有保护应激性溃疡作用是否影响与应激性溃疡相关的其它因素，还有待进一步深入研究。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。