胁迫的

50Mmol/L NaCl stress had no influence on level of ATP in root of Ginkgo andAilanthus.Level of ATP in roots of Ginkgo,Ailanthus and Robinia decreased greatly under200mmol/L NaCl stress.The extent of decrease of ATP level accord to plant salt tolerance:Ginkgo is more salt tolerance,and ATP decreases more slowly than that of Ailanthus andRobinia.

11.50mmol/L的NaCl胁迫没有引起银杏和臭椿根尖ATP水平的明显改变；200mmol/L的NaCl胁迫引起银杏、刺槐和臭椿ATP水平指数性的衰减，ATP的下降幅度的变化与植物耐盐性一致：耐盐性强的银杏，ATP的下降幅度明显比刺槐和臭椿小。

The main results are shown as follows:1.Screening freezing tolerant cultivars and lines of chrysanthemumTwenty seven lines of chrysanthemum grown outdoors were screened using \'injury rate\' and \'injury index\'.There were 7 cultivars and lines,which were relatively high tolerant to freezing treatment,i.e.,\'Jinbuhuan\',\'Xuetao\',\'Fuli\',\'Donglin 4\',\'Donglin 6\',\'Donglin 9\', and \'Donglin 13\'.There were 3 cultivars and lines,which were less tolerant,i.e.,\'Fenmeigui\', \'Donglin 1\',and \'Donglin 2\';The determination of electrolyte leakage,and contents of free proline,MDA,CAT,fructose,glucose and soluble sugars on 7 lines of chrysanthemums at different stages freezing showed that \'Xuetao\' and \'Donglin 4\' were more tolerant than others, and that especially \'Donglin 4\' was able to regulate its osmotic pathway to alleviate the \'injury degree\' caused by freezing conditions.2.Protein expression profile of chrysanthemum under low temperature stressTwo-dimensional liquid chromatography was applied to fractionate and analyze total protein taken from \'Donglin 4\' leaves grown at low temperature and at normal condition. Chromatography peaks of fraction were transformed to gel graph by ProteoVue software.

主要研究结果如下：1、露地菊抗冻品种和品系的筛选利用受伤率和伤害指数两项指标对27个露地菊品系进行耐低温筛选，筛选出'金不换\'、'雪涛\'、'富丽\'、'东林4号\'、'东林6号\'、'东林9号\'、'东林13号\'等7个耐低温品系，同时发现'粉玫瑰\'、'东林1号\'、'东林2号\'三个品系的耐低温能力最差；通过对不同自然低温处理时期，7个品系的电导率、丙二醛含量、脯氨酸含量、过氧化氢酶、果糖、葡萄糖和可溶性糖等指标的测定发现，'雪涛\'和'东林4号\'的耐低温能力要强于其它品系，尤其是'东林4号\'对低温的调节能力较强，在低温胁迫下，能够调节渗透途径减轻低温的伤害。2、低温胁迫下，露地菊蛋白质表达谱分析利用二维液相色谱技术，分离了'东林4号\'露地菊品系的低温处理和对照样品的总蛋白，通过ProteoVue软件分析发现：处理样品与对照相比共有蛋白差异点36个，其中显著增强表达的蛋白差异点有26个。

The results showed that the seed germination and stem apexes growth of the materials was decreased with the increase of NaCl concentration and PYM concentration and treating time.

结果表明：供试种子的萌发率和茎端组织的存活率随培养基中NaCl浓度的上升急剧下降，平阳霉素处理对茎端组织存活率的抑制明显大于对种子萌发率的影响，平阳霉素处理后存活的种子和茎端组织经NaCl胁迫筛选，筛选出耐NaCl胁迫力明显提高的存活苗，存活苗经无性繁殖，可以保持较高的耐NaCl能力。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Before fissuration, osmotic adjustment occurred by the addition of solute to cells and decreasing the loss of water in the body, so that it could maintain normal turgor pressure during drought. After fissurate growth, turgor pressure was maintained by higher cell and tissue elasticity lower ε(superscript max and by osmotic adjustment for normal plant development in dry conditions and to avoid dehydration under water stress during the drought period, but it also became more sensitive to water stress. The ability to maintain turgor pressure varied from strong to weak during the period before, during and after fissuration. Fissurate growth decreased the ability of P. mongolica to maintain turgor pressure and led to changes in walls related to drought tolerance.

结果表明：1未劈裂绵刺主要通过增加细胞内溶质，减少细胞内的水分丧失来进行渗透调节，从而在干旱胁迫下能够维持正常的膨压。2己劈裂绵刺通过渗透调节和高的组织弹性两条途径来共同保持膨压，以抵抗不良的生存环境；同时对环境水分胁迫具有较高的敏感性。33种状态绵刺保持膨压的能力由强到弱依次为：未劈裂绵刺、正在劈裂绵刺、已劈裂绵刺。4劈裂的发生导致绵刺保持膨压能力的降低，同时耐旱方式和途径发生了变化。

Proline was much more accumulated in the fleshy leaves of K.foliatum with increased salt concentration concided with the expression pattern of K.foliatum P5CS, suggesting that proline and betain as two effective osmolytes had important functions for Kalidium foliatum during stress.

检测的各基因均受盐的诱导。P5CS和KfBADH基因的表达随着盐浓度的升高而表达逐渐增强，而脯氨酸的含量也随着盐胁迫程度的增强而在盐爪爪肉质化叶内积累更多，说明脯氨酸和甜菜碱作为重要的渗调物质，在植物逆境胁迫中可能发挥着重要的作用。

In this study,under laboratory conditions,using traditional microbiological culture method,select the Pb2+ stress training of Pistia stratiote and Myriophyllum spicatum L. under water culture, through the changs of root- microbial of Pistia stratiote and Myriophyllum spicatum L.,the results show that bacteria,fungi,actinomycetes have different concentrations under Pb2+ stress. It is specificity of ammonification, nitrification,andanti-Nitrosation after Pb2+ stress.

中文摘要：本研究在实验室条件下，用传统的微生物培养法，选择重金属离子Pb2+在水培条件下对大薸和穗状狐尾藻进行胁迫培养，通过测定大薸和穗状狐尾藻根系的微生物区系及与氮循环有关的四种细菌的数量变化发现：细菌、真菌、放线菌对重金属Pb2+的不同浓度的敏感性不同；重金属Pb2+胁迫对氨化、硝化、亚硝化和反硝化四类细菌的影响也表现出各自的特殊性。

Var. pyramidalis is not positively correlated with ion concentration in growth environment, the capacity of selective absorption and transport of ions in Populus alba L. var. pyramidali under salt stress in drainage canal is higher than Populus alba L. var. pyramidal is of normal growth in irrigation canal. Under salt stress, the concentration of K(superscript +)、Na(superscript +) of roots and leave of Populus alba L. var. pyramidalis have changed, the concentration of K(superscript +) of roots and leave dropt the concentration of Na(superscript +) of roots markedly increased, the concentration of Ca(superscript 2+), Mg(superscript 2+) have slightly changed, the concentration of Ca(superscript 2+) of roots dropted, the concentration of Mg(superscript 2+) increased, the concentration of Ca(superscript 2+), Mg(superscript 2+) of leave increased.

主要结论为：新疆杨各器官的离子含量与生长环境中的离子浓度不存在正相关趋势；排渠盐胁迫下生长的新疆杨对离子选择吸收和运输的能力高于灌渠正常生长的新疆杨；盐胁迫下，新疆杨根和叶中K、Na的浓度发生了明显的变化，K浓度在根和叶中呈下降趋势，Na浓度在根中明显增加，根和叶中Ca(上标 2+)、Mg(上标 2+)浓度的变化趋势略缓，根中Ca(上标 2+)浓度下降，Mg(上标 2+)浓度增加，叶中Ca(上标 2+)、Mg(上标 2+)浓度有所上升。

Results showed that, with cadmium concentration and stress time increasing, the chlorophyll content in the leaves of the three fruit tree species declined, and the decreasing range was in the order of apricot, plum, and pear from high to low; relative conductivity of the leaves showed an increasing tendency; MDA content first decreased and then increased; POD activity first increased and then decreased; proline content also exhibited an increasing tendency.

结果表明：3个树种随着重金属质量分数和胁迫时间的增加，各树种叶片的叶绿素质量分数降低，其中杏树的降幅最大，李树居中，梨树最小；相对电导率有逐渐升高的趋势；叶片MDA质量摩尔浓度均出现先降低后升高的趋势；POD活性随土壤中镉质量分数的增加先升高后降低；脯氨酸质量分数随着重金属胁迫时间的延长有不断积累的趋势。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。