胁迫的

According to the investigation on the local situation of aquatic vegetation in Littoral Regions of Gonghu Lake and Meiliang Bay of the Wuxi Taihu Lake, the species of aquatic vegetation, community forms and biomass were acquired. Then a contrast of vegetation distribution characteristic between the two Littoral Regions was analyzed which induced to the analysis of impact factors. In the treatments designed as varying degree oxidation intimidate, the heavy metal cadmium and the ammonia nitrogen single and compound poisons, the COD sewage cultivate, the plant growth opsonin processes, the effect of the intimidate environment on the submerged plant growth and the physiological biochemistry characteristic was analyzed. The effective method to improve the resist adversity of submerged macrophyte was discussed. The results will give a suggestion to the restoration of the submerged macrophyte, the efficiency and reconstruction in Lake Ecosystem.

通过对无锡太湖两重要湖湾贡湖和梅梁湖湖滨带沿岸浅水区水生植被现状进行调查，了解了水生植物的种类、群落组成及生物量，对两湖湾湖滨带沿岸浅水区水生植被分布特征进行了对比，并进行了影响因素分析；通过不同程度的氧化胁迫、重金属镉与氨态氮单一和复合作用、高COD生活污水胁迫培养、植物生长调节素处理，分析胁迫环境和壳聚糖各自对沉水植物生长的影响及作用机理，探讨提高沉水植物逆境抗性的有效措施；探寻沉水植物修复的理论依据和有效途径，为急待进行水生植被恢复的湖泊提供依据和建议。

To study the ecophysiological effects of low temperature stress on podocarpus fleuryi Hickel seedlings, two-year-old seedlings were selected as experimental material. We conducted comparative study. On the one hand, seedlings were separately placed in the plots located at the top, half-way-up and the foot of Jinyun Mountain (different altitudes at Jinyun Mountain led to the difference in air temperature) and subjected to winter low temperature stress. On the other hand, in laboratory, seedlings were exposed to simulated low temperature stress which were artificially controlled .

为了探讨低温胁迫对长叶竹柏幼苗的生理生态影响，本试验选取长叶竹柏的二龄幼苗为试验材料，在试验方法上采取了对比研究方法：一方面，利用缙云山不同的海拔造成的温度差异，分别将幼苗置于山顶、山腰和山脚下三个样地中，进行野外冬季自然低温胁迫处理；另一方面，在实验室中，利用人工控制的不同低温进行模拟低温胁迫处理。

The results showed that the rate of photosynthesis, the contents of individual and total carotenoids and the pool size of xanthophyll cycle decreased with age increasing of the leaf.

结果表明：随着叶片的衰老，光合速率、类胡萝卜素不同组分及总的类胡萝卜素含量和叶黄素循环库下降；不同叶龄的叶片经高光胁迫后，第 5叶qN增加的幅度比第 6叶和老叶(第 3和 4叶)大；与高光胁迫前相比，第 3、4、5和 6叶光系统Ⅱ激发压(1-qP)分别增加了 4 4 %、5 7%、19%和 4 5 %；第 5叶具有高胡萝卜素含量和高紫黄质到玉米黄质的转化，这与其呈现较强的抗高光胁迫相一致。

The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.

本项目的主要研究结果如下：（1）ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中，质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性；（2）ABA诱导的H2O2产生活化一个46kDa的MAPK，由此而导致编码抗氧化防护酶基因的表达与酶活性的上调；而MAPK的活化也能增强H2O2的产生，从而形成一个正的反馈调节环；（3）水分胁迫也能诱导一46kDa MAPK活化，这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生，同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调；（4）ABA诱导一个依赖于H2O2的NO产生，NO活化一个46kDa的MAPK，从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调；同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明：(1)在膜透性的研究中，丛毛羊胡子草抗寒性最强，采用电导率法测得半致死温度LT_(50最低，丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃；在可溶性蛋白含量研究中，抗寒锻炼前后三种植物的可溶性蛋白含量均增加，同时采用SDS-PAGE法，通过对蛋白条带分析，以丛毛羊胡子草条带数最多且着色深；(2)在可溶性糖的研究中，三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高，且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势，丛毛羊胡子草可溶性糖含量下降较小；从游离脯氨酸含量可知，低温的胁迫影响了植物叶片游离脯氨酸的含量变化，植物的抗寒性与游离脯氨酸有关；低温胁迫下三种植物叶绿素含量随着温度的下降，叶绿素含量、叶绿素a、叶绿素b和叶绿素a／b均呈下降趋势，狗牙根对低温胁迫最为敏感；(3)低温锻炼前后，丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的，特别是过氧化物酶，说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

Trends of overground physiological and biochemical indexs are almost the same—weak soil Naphthalin pollution stress stimulate them,strong pollution restrain stress them. In the first two weeks of seeding stage weak soil Naphthalin pollution stimulate root activity,but soil Naphthalin pollution stress restrain root activity from the second week till jointing stage, In corn grouting stage soil Naphthalin pollution stress stimulate root activity.

部分的生理生化指标都有促进作用，而高浓度的土壤萘污染胁迫对玉米地上部分的生理生化指标有抑制作用；苗期的前两周低浓度土壤萘污染胁迫对玉米的根系活力有促进作用，苗期第三周到拔节期土壤萘污染胁迫抑制玉米的根系活力，而到了用营养生长到以生殖生长为中心的灌浆期土壤萘污染对玉米的根系活力有促进作用。

The result of BLASTx showed that Maasr1 was homologous with Oryza sativa abscisic acid-inducible and stress-inducible protein (Asr1) mRNA (86%),Saccharum officinarum SoDip22 mRNA for drought inducible 22 kD protein (84%),Lilium longiflorum anth (A23) mRNA (86%), and Prunus armeniaca abscisic stress ripening protein homolog mRNA(82%).

用Maasr1基因作BLASTn比对发现它可能与水稻ABA和胁迫诱导蛋白(ASR1)的mRNA有86%的同源，与甘蔗干旱胁迫蛋白(SoDip22)的mRNA有84%的同源，与百合花A23基因的mRNA有86%的同源，与野黑杏脱落胁迫成熟蛋白的mRNA有82%的同源。

The results showed that C. chinensis net photosynthetic rate, transpiratory rate, stomatal conductance were dereased with the increase of stress and the degress of the decrease were the least ones urder light water stress concentrations of intercellular CO2 decreased with the increase of stress. Seedlings of C.

结果表明：紫荆净光合速率、蒸腾速率、气孔导度随着胁迫强度增加呈现出降低的趋势，但在轻度水分胁迫时的降低最少；紫荆的细胞间隙CO2浓度随着胁迫强度的提高呈现出升高的趋势。

Applied with test of potted plant and measurement absorbency for study physiological response of activity of cell defense enzymes and lipid peroxidation of slash pine's half-sib under water stress. There are 7 families comparison with general slash pine. The contents of superoxide dismutase, catalase, antioxidant, malondialdehyde and the rate of superoxide anion(-O2) generating are mensurated under normal water condition while soil water content is 70% and light water stress while soil water content is 55% to 60% and medium water stress while soil water content is 35% to 40% and heavy water stress while soil water content is 20% to 25%. The test result indicate that either water grads or families of contents of SOD,CAT,AsA,MDA are mighty notable difference. The rate of -O2 generating is notable difference within families. The rate of -O2 generating is mighty notable difference within water grades.

为研究湿地松优良半同胞家系保护酶及脂质过氧化作用对水分逆境的生理响应，采用盆栽试验和吸光度测定结合的方法，以普通湿地松种子为对照，测定了七个湿地松半同胞家系在正常水分状态（土壤相对含水量为70%）、弱度水分胁迫（土壤相对含水量为55%~60%）、中度水分胁迫（土壤相对含水量为35%~40%）和强度水分胁迫（土壤相对含水量为20%~25%）条件下的SOD、CAT、AsA、MDA的含量和-O2产生速率，研究结果表明：无论是不同水分梯度还是不同家系水平，SOD、CAT、AsA活性和MDA含量的差异均达极显著水平，不同家系水平间-O2产生速率有显著差异，不同水分梯度间-O2产生速率差异达极显著水平。

As one of the most important osmoregulation actor, firstly,Proline plays a keyrole in the osmotic potential adjustment in plant cells. Secondly, Proline can preventNaCl from damaging the structure of biomacromolecule in cells. Thirdly, the highdissolubility and non-rejection capability of Proline can enlarge the solution volumnof the cell, so the concentration of salt will be diminished in cytoplasm and the saltstress will be relieved. Forthly, the accumulation of Proline could prevent thedehydrolysis from sap cavity in cytoplasm. Fifthly, the synthesis of plant chlorophyllwill be diminished under the salt stress, but the Proline can provide request ofchlorophyll synthesis.

脯氨酸作为渗透调节剂对植物体细胞渗透势的变化起重要作用：一，在盐胁迫下，脯氨酸在植物体内是一种重要的渗透调节剂，它是一些高等植物和绿藻抗盐和抗旱的主要渗透剂之一；二，脯氨酸可以保护细胞中的生物大分子的结构，使之不被NaCl破坏，并能维持其完整的水合范围；三，脯氨酸的高度溶解性以及其对植物各种酶活性不抑制性，可以扩大细胞的溶解容积，从而降低细胞质液中盐的浓度，减轻盐的胁迫作用；四，细胞质中积累的大量脯氨酸可以防止液泡对细胞质的脱水作用；五，在盐胁迫下，植物叶绿素的合成受到抑制，叶绿素的合成需要脯氨酸。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。