肿瘤

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Intradermal tumor model of B16 cells in C57BL mice is helpful to monitor tumor growth in early and middle stages and is one of ideal models in tumor studies.

B16细胞皮内移植瘤模型易于对肿瘤生长的观察和测量，是早、中期肿瘤研究的合适模型之一。

Intraperitoneal tumor spreading involves the interaction between the tumor cells, peritoneum, and the extracellular matrix around them.

腹腔内的肿瘤扩散，与肿瘤细胞、腹膜、细胞外基质之间的交互作用有关。

Abstract］ Objective To study the early diagnosis and surgical treatment of intraspinal tumor.

目的 探讨椎管内肿瘤的早期诊断及手术治疗方法方法对2002～2006年在我院手术治疗的42例椎管内肿瘤患者的病例资料进行分析，其中男22例，女20例，年龄17～66岁，平均36岁。

There were 31 cases of intraspinal tumors. Conclusions Astrocytoma, embryonal tumors and craniopharyngioma are the most common types of CNS tumors in childhood.

椎管内31例。结论星形细胞瘤、胚胎性肿瘤和颅咽管瘤是最常见的儿童CNS肿瘤组织学类型。

The essential events of the process are detachment, intravasation, circulation, arrest, extravasation, angiogenesis and proliferation, which come down to interactions of neoplastic cells with host cells and extracellular matrix -governed by adhesion, migration, invasion, proteaselysis, and the complicated signal transduction.

而所有步骤均可归结为癌细胞与宿主细胞及细胞外基质(Extracellular Matrix，ECM)之间的相互作用——粘附、浸润、移动、蛋白酶分泌以及复杂的信号转导过程，基于细胞信息传递和调控在细胞生命活动中处于主导地位，癌细胞信号转导系统的作用及相关机制对于揭示肿瘤转移的本质，探索新的抗肿瘤转移途径具有十分重要的作用。

The most useful indications for the specific diagnosis of intraventricular tumors are location of the tumor within the ventricles, and age of the patient

对于脑室内肿瘤的定性诊断，最有用的指征是肿瘤在脑室内部位和病人的年龄

Mouth neoplasm; jow neoplasm; pathology, oral

口腔肿瘤颌肿瘤病理学，口腔

Tumor nests were mostly composed of large atypical cells with clear cytoplasm containing UEA-I positive, PAS-positive, and PAS-diastase labile materials. Some of the nests showed tricholemmal-type keratinization. Tricholemmal carcinoma was impressed.

部分肿瘤组织显示出外毛根鞘性角化，其肿瘤组织主要由内含澄清细胞质之非典型大型细胞所组成，其具有UEA-I阳性，PAS阳性而PAS-D阴性之特性，临床诊断为外毛根鞘癌。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。