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Keywords : clubroot; pharmacy screening; control effect

中文关键词:根肿病;药剂筛选;防治效果

Fifty-three primer combinations were found to amplify nuclear DNA of Chinese cabbage, properly. They amplified the fragments up to 4 381 with an average of the 83. Among them, 9 markers derived from 9 primer combinations were linked to the clubroot resistance gene.

筛选出的适宜53对引物组合共扩增出4381条带,占总扩增片断的33.50%,平均每对扩增出83条,其中9个组合扩增出与抗根肿病基因连锁标记。

Mixed drug and drench were used to evaluated the control efficacy of 10 kinds of fungicides on clubroot of Chinese cabbage by pot experiments. Three pharmaceutical drugs selected by potted experiment were carried in field experiment.

中文摘要:分别采用拌药法和灌药法,比较了10种供试药剂对白菜根肿病的温室防治效果,并对盆栽试验防效较好的3种药剂进行了田间药效试验。

The result indicated that 237 combinations amplified a total of 13 079 AFLP fragments with an average of 55 fragments, the average of 1.30 markers per 1 000 AFLP fragments were found to be linked to clubroot resistant gene; the amplified polymorphic fragments were 3 167 between the two parents, account for 24 percent of polymorphic frequency; the number of amplified fragments and the number of polymorphic fragments was in positive linear correlation; GC content of AFLP primer combinations significantly affects the number of amplified fragments and the number of polymorphic fragments, indicating negative linear correlation between them.

结果表明:平均每个组合检测到55条带,共扩增13079条带,平均每1000条带检测到1.30个根肿病抗性基因连锁标记;多态性带数为3167条,双亲间多态性频率约为0.24;扩增带数与多态性带数呈线性正相关,引物组合中的不同GC含量与扩增带数和多态性带数存在显著性差异,并表现出线性负相关。

A biocontrol strain, XF-1, was identified as Bacillus subtilis based on morphology, physiology, biochemistry and biotechnology, which could control clubroot of cruciferous crops effectively.

依据传统形态学、生理生化特征及16S rRNA基因序列,将一株对十字花科根肿病有很好防治效果的菌株XF-1鉴定为枯草芽孢杆菌。

In this study the clubroot resistant CR DH line, susceptible inbred line 94SK of Chinese cabbage, the resistant and susceptible DNA bulks constructed from F2 individuals of CR DH line and 94SK were used to select the adaptive AFLP primer combination. AFLP analysis was performed with 256 Pst Ⅰ/Mse Ⅰ primer combinations.

为了筛选出大白菜适宜的AFLP引物组合,研究以抗根肿病大白菜双单倍体CR Shinki、感病自交系94SK及其后代F2构成的纯合抗病和纯合感病DNA混合池为材料,利用256对Pst Ⅰ/Mse Ⅰ引物组合进行了AFLP分析。

On semi-solid medium, P. brassicae invaded into cells of cut surface of root segments and made the cut surface swell.

在半固体培养基内,根肿菌侵入根段切口处的细胞内,并很快使切口处膨大起来。

It\'s an environment that is at once trashy and highly exacting: every hangnail a sin, every eye-bag a crime.

在这样一种荒诞无稽又高度苛刻的氛围下,一根指尖的倒刺就是一种罪过,一个肿肿的眼袋就是一种犯罪。

Methods ABC method was applied to detect ER and PR in 20 healthy persons (10 males and 10 females),as well as in 58 patients,of which 14 cases were dental pulp polypus,20 were apical cysts,and 24 were periapical granuloma.

应用ABC法对20颗健康牙髓作为对照组及14例牙髓息肉、20例根尖囊肿和24例根尖肉芽肿作为实验组做ER、PR检测。

Methods:①Pharmacodynamics observed the effects of The root and leaf of Panicled Fameflower on swimming time of mouse, and physiological function, IL-2 IgA IgM IgG C3 C4 of rat of asthenia of the spleen, and The leaf of Panicled Fameflower on determinating mininal inhibitory concentration, swelling of mouse ear, capillary vein permeability of mouse skin, granuloma in rat.

①药效学实验:观察土人参根、叶对小鼠游泳时间的影响,以及对&脾虚&大鼠脾脏生理功能,细胞因子IL-2、IgA、IgM、IgG、C3、C4的影响,验证土人参根、叶&健脾&功效;观察土人参叶体外抗菌实验,对二甲苯致小鼠耳廓肿胀的影响,对小鼠皮肤毛细血管通透性增高的影响,以及对大鼠棉球肉芽肿的影响,验证土人参叶&解毒消痈&功效。

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