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Results: When arcuate and interlobular arteries were detected, RI of CKD patients in each stage increased significantly compared with the control group, and RI of interlobular arteries of mild renal dysfunction patients were different from those of moderate and severe renal dysfunction patients significantly.

结果:各期CKD患者弓形动脉、小叶间动脉砒均与健康对照组有显著性差异(P〈0.05);CKD患者轻度肾损害小叶间动脉砒较中重度肾损害有显著性差异(P(0.05);应用eflow显像对小叶间血管的分型分析表明CKD各期的小叶间血管较健康对照组均有减少,差异有显著性意义,且轻度肾损害小叶间血管较中重度肾损害丰富,差异有显著性意义。

Methods: This study was approved by the Department of Nephrology, the second hospital of Hebei Medical University. We choose 36 cases of IgA nephropathy(25 males and 11 fema- les).The mean age of them was 35.0±12.6 years.They were defi- nited by renal biopsy from July 2008 to Feb.2009.Patients with hypertension, diabetes mellitus, Lupus Nephritis, HBV-GN associated glomerulonephritis,thyropathy,renal damage induced by polyarthritis destruens,neoplasms associated nephropathy and those on glucocorticoid,immunosuppressive agents,enoxa- parin sodium and angiotensin converting enzyme inhibitor be- fore renal biopsy were excluded from this study.

河北医科大学第二临床医学院肾内科于2008年07月到2009年02月间经肾活检确诊的IgA肾病患者36例(其中男性25例,女性11例,年龄35.0±12.6岁),临床上除外高血压肾损害、紫癜性肾炎、乙肝病毒相关性肾炎、狼疮性肾炎、甲状腺病相关性肾损害、类风湿性关节炎肾损害、肿瘤相关性肾病等继发性肾脏病患者,并除外肥胖、妊娠、糖尿病、肝脏疾病、药物等导致的肾损伤及急性间质性肾炎等患者。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。

Sun yikui excelled in using the medicine which can supply qi to restore yang , Applicating the flesh and blood of animals but didnlt give up the herbaceous medicine and adjusting the yin and yang of the body by means of the medicine which can tranquilize spiritual and reinforce premodial qi.

孙一奎在医学理论和临床实践方面的卓越成就,对后世产生了深远的影响,使后世医家认识到以温补鼓动肾间动气和培元固本,保护三焦元气的重要性。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达,阳性率分别为57.1%(8/14)和46.2%(6/13),提示WT1基因在胚胎肾分化发育的过程中起着重要作用,WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子,其表达在时间上和空间上都受到严格的调控,WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17),阳性部位在胚基型和上皮型肿瘤细胞核,表达部位和阳性率与早期胚胎肾相似,其中间质型肾母细胞瘤均为阴性,胚基型和上皮型肾母细胞瘤阳性率70%(7/10),两组间阳性率有显著差异。

Theurinary IL-6 level positively correlated with density of glomerular matrix membrane, global sclerosis, fiber or fibrocellular crescents and interstitial fibrosis (p. 05). According to the degree of density of glomerular matrix membrane and interstitial fibrosis, urinary Col-IV level had better correlation than urinary TGF-betal and IL-6 levels.In IgAN, Col-IV showed increased expression in diseased renal tissue whereas the site of expression of TGF-betal was mainly localized within the cytoplasm of tubular epithelial cells. Interstial expressionwas also present but glomerular TGF-betal expression was found only in patients with heavy mesangial proliferation. There was a significant correlation between glomerular positivity for Col-IV and severity of histological damage. There was also a significant correlation between positivity for TGF-betal and Col-IV in the tubular epithelial and interstitial lesions. In contrast, there was no ralationship between glomerular positivity for TGF-betal and severity of histological damage.The urinary TGF-betal level paralleled tubular TGF-betal expression.

结果 ①IgAN患者尿TGF-β1、IL-6、Col-Ⅳ水平较健康人明显增高(P<0.01),该变化与血中的浓度无关(P>0.05);②尿TGF-β1水平与小管间质TGF-β1阳性表达呈正相关(P=0.000),而与小球TGF-β1阳性表达无关(P>0.05),尿Col-Ⅳ水平与小球和小管间质Col-Ⅳ阳性表达均呈良好的相关性(P<0.01),还与小管间质TGF-β1阳性表达呈正相关(P<0.05):③小球Col-Ⅳ阳性表达与肾组织慢性病变密切相关(P<0.05),小管间质Col-Ⅳ和TGF-β1阳性表达均与肾小管间质病变呈良好的相关性(P<0.01),而小球TGF-β1阳性表达与肾组织损伤无关(P>0.05);④尿TGF-β1、Col-Ⅳ水平与肾小球基质基底膜面密度、小管间质病变呈正相关(P<0.01),与小球内细胞数呈负相关(P<0.05),该结果与其在组织中的表达一致;尿IL-6水平浙江大学硕士学位论文尿TGF一B一、IL一6和Col一IV在IgA肾病中的应用价值与基质基底膜面密度、球性硬化、纤维或细胞纤维新月体所占肾小球百分数及小管间质病变均有显著的相关性(F.05);在轻度肾病理损伤时,尿'l'G卜pl、I卜6、Col一IV水平即升高,而尿Col一W在反映细胞外基质积聚和间质纤维化程度上比尿TGF一pl和IL一6有更好的相关性。

Were used to detect the experimental group and control group, supine position when the left renal vein compression section diameter of the expansion of paragraph a, b, left renal vein peak velocity pressure above Va, the pressure gradient, pressure, as well as the expansion of left renal vein peak velocity Vb paragraph and to observe the two forms of the spectrum, and then testing all the main subjects with renal renal artery, interlobar artery and renal blood flow velocity of the main renal artery resistance index; record of the experimental group urine test subjects in the number of red blood cells.

分别检测实验组及对照组平卧位时左肾静脉受压段、扩张段内径a、b,左肾静脉受压段峰值血流速度Va、压力阶差、压力,以及左肾静脉扩张段峰值血流速度Vb并观察两处的频谱形态,再检测所有受检者双肾主肾动脉、叶间动脉的血流速度及双肾主肾动脉阻力指数;记录实验组受检者尿常规化验中红细胞数量。

OBJECTIVE: To observe the in vivo location and differentiation of 4',6-diamidino-2-phenylindole and green fluorescence protein-labeled cells in adriamycin nephrosis rats so as to explore an efficient labeling and tracing method for metanephric mesenchymal cells derived from embryonic rats.

目的:观察DAPI及绿色荧光蛋白标记细胞在阿霉素肾病大鼠体内的定位及分化,探讨便捷的大鼠胚胎后肾间充质细胞的体外标记方法。

The mouse was carried out half nephric duct ligation to induce the renal interstitial fibrosis animal model,afterwards,to set up three groups,control group,sham operation group(intragastric administration with normal sodium according to 10ml/kg weight),treatment group...

采用小鼠单侧输尿管结扎法诱导肾间质纤维化的动物模型,用盐酸青藤碱治疗,并设对照组、假手术组(小鼠每日按10m l/kg体重用生理盐水灌胃,直至治疗结束)、治疗组(选用正清风痛宁缓释片按每日30mg/kg体重给小鼠灌胃,连续3周,),检测血尿素氮、血肌酐、血清层粘连蛋白、纤维连接蛋白的含量,免疫组化法测肾脏组织转化因子β1(TNF-β1)的表达并进行肾脏病理学的观察。

A small segment of aorta is seen between the normal, smooth-surfaced kidneys.

表面光滑的正常两肾间,可见一小段主动脉。1/150的人会出现部分或全部的单侧或双侧的双输尿管。

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