肽酶

Proteinases and peptidases are involved in the hydrolysis of protein during digestion, and have...

蛋白质酶和肽酶在消化过程中参与蛋白质的水解，而且具有重要的生理学和病理学作用。

From the coelomic fluid of Nereis virens, a novel and single chain serine protease with more strong fibrinolytic activity is discovered. The fibrinolytic protease has a medical value for preventing and treating thrombosis.

Nereis virens的体腔液中存在一种纤溶活性极强的单链丝氨酸内肽酶，该纤溶酶对预防和治疗血栓性疾病具有一定的药用价值。

Our group has developed a GDEPT system that uses the bacterial enzyme carboxypeptidase G2 to convert nitrogen mustard prodrugs into potent DNA crosslinking agents, and a clinical trial of this system is pending.

我们研究组发展出了一个GDEPT系统，其可以使用细菌酶羧肽酶G2来将氮芥前药转化成有效的DNA交联剂，而针对该系统的一个临床试验也即将开展。

There were twelve different hepatocyte proteins interacting with HCBP12 by Yeast two-hybrid, containing human sapien apolipoprotein C-I/B, human sapiens mitochondrion, human sapiens metallothionein 2A, human sapiens beta-2-microglobin,human sapiens carboxylesterase, and human sapiens serpin peptidase inhibitor, with known function and one with unknown function.

利用酵母双杂交技术筛选出12个与HCBP12相互作用的蛋白，其中包括人类载脂蛋白C-I/B、人类线粒体蛋白、人类金属硫蛋白2A、人类β-2微球蛋白、人类羧酸脂酶4和人类丝氨酸肽酶抑制剂等已知功能蛋白及1个未知功能蛋白。

PNAs are uncharged DNA mimics, which are resistant to nucleases and peptidases, and hybridize with complementary DNA and RNA with high affinity.

PNA是一类不带电荷的DNA相似物，不受核酸酶和肽酶的作用，可以和cDNA、RNA高亲和性杂交。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.

结果：术后无上腹部绞痛、高热，黄疸逐渐减退，每日引流胆汁300ml-1000ml不等，由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁，腹腔引流通畅，引流液为淡红色，量从最初的200ml迅速过渡到10ml，血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常，血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降，术后12d夹闭T管，术后24d T管造影提示左、右肝管及胆总管下端通畅，无狭窄。

Results Patients who had at least one abnormal result of liver function test accounted for 70.99% in untreated group and 61.07% in treated group. The most common abnormal hepatic function parameter in patients with thyrotoxic hepatic lesion was increased alkaline phosphate enzyme, which was 57.3% in untreated group and 51.0% in treated group. Abnormalities of alanine transaminase, aspartate transaminase, gamma-glutamyl tyanspeptidase and total bilirubin were 28.2%(37/131), 18.3%(24/131), 10.7%(14/131) and 21.4%(28/131) in untreated group and 21.5%(32/149), 15.4%(23/148), 4.7%(7/149) and 20.1%(30/149) in treated group, respectively. The possibility of thyrotoxic hepatic dysfunction was correlated with the patients' age and FT3 and FT4 levels, but not with the genesis and course of disease, sex, family history of hyperthyroidism, or levels of TGAb and TMAb. Conclusion Abnormal results of liver function test are commom in patients with hyperthyroidism.

结果 在未治组甲亢性肝功能损害的发生率为70.99%，经治组为61.07%；甲亢性肝功能损害最常见的异常指标是碱性磷酸酶增高，未治组、经治组分别为57.3%、51.0%；血清谷丙转氨酶异常在两组的发生率分别为28.2%、21.5%；血清谷草转氨酶异常在两组的发生率分别为18.3%、15.4%；总胆红素异常在两组的发生率分别为21.4%、20.1%；谷酰胺转肽酶异常的发生率未治组和经治组分别为10.7%、4.7%；甲亢性肝功能损害的发生与病程、甲状腺疾病家族史、性别、TGAb水平、TMAb水平无关，与年龄、甲状腺激素水平有关。

Fourteen weeks later all the rats were sacrificed underwent serological test and immunohistochemical stain of liver. Results Serum level of γ-Glutamyltransferase, alkaline phosphatase, bile acid were significantly higher in DEN exposure rats than those in normal rats, and radiated rats were lower than unradiated rats, but has no significant difference.

结果诱导的肝癌大鼠，血清中γ-谷氨酰转肽酶、碱性磷酸酶、胆汁酸较正常大鼠组明显升高，毫米波照射肝癌大鼠γ-GT、AKP、BA与肿瘤对照组相比略下降，统计学上各组间无明显差异。

Result VPI simultaneously inhibits ACE and neutral endopeptidase. Conclusion VPI may be more effective than A ACEI in the treatment of hypertension and heart failure.

结果 VPI同时具有双重抑制中性内肽酶和血管紧张素转换酶的作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。