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METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后,又通过DEAE离子交换层析纯化包涵体,包涵体在8 mol/L尿素变性溶解,在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中,通过简单的稀释复性,通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2,最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

Methods The specific activity of pancreatic kininogenase in the eluant was monitored at different time to determine the scope of collection of the eluant.

方法通过监测不同时间点洗脱液中胰激肽原酶的比活,确定洗脱液的收集范围。

Indeed a great article…Jill… great things u have done… that u turn a fiction book tat try to harms fellow christian faith into something that make us so eargerly strengthen and proclaim our faith… well… thats y ppl say the rigthteous one will win..

文章的确很伟大的事情::建筑业::::美美做图书达试图把小说害研究员基督教信仰,使我们变得这么强,宣示eargerly信念::::好脂肪酶肽Y说thatsrigthteous一赢。。

Corn gluten meal; antihypertensive peptides ; angiotensin converting enzyme

玉米蛋白;降血压肽;血管紧张素转换酶

The recombinant proteins were purified with affinity columns by the 6 histidines tag or Glutathione-S-Transferase they were fused to.

并根据所表达融合蛋白的6个组氨酸或谷胱甘肽-S-转移酶标记进行亲和纯化。

On the other hand, epidermal growth factor , calcium ionophore (A23187), kallikrein and tumor promoter (phorbol-12 myristate-13 acetate) have been shown to stimulate the synthesis of prostaglandins from the thymic epithelial cells.

在另一方面,表皮生长因子、钙离子载体(A〓3817)、激肽释放酶和肿瘤启动子却能促进胸腺内分泌上皮细胞中前列腺素的合成。

Many factors such as pancreatic enzyme, polymorphonuclear neutrophil, oxygen free radical, cytokine, microcirculatory disturbance, complement, kinin, NO and ET play an important role in the pathogenesis of SAP by interacting with each other.

胰酶、中性粒细胞、氧自由基、细胞因子、微循环障碍、补体系统、激肽、一氧化氮以及内皮素等因素,通过彼此的相互作用和影响,在SAP发病机制中起着十分重要的作用。

The data on the lysosomes suggested that the lysosomes involved in certain events in endocrine cells as hormone's synthesis, degradation and so on.

国外的一些资料表明,溶酶体可能在激素合成、降解等多个环节参与了内分泌细胞的功能活动,但是大部分工作限于分泌肽类激素的细胞。

HGF is 2,000 nanograms per ml of a micro-dilution recombinant hybrid Biotropin "The real thing"+ amino acid-polypeptide precursors, Arginine pyroglutemate, Lysine HCL, Glycine and L-Glutamine.

HGF微型稀释液每毫升约含2000毫微克生长因子,稀释液的成分有&真正的生长因子&+氨基酸肽前体,精氨酸酰胺酶,赖氨酸盐酸,甘氨酸和L -谷氨酰胺。

POur enzyme production line lives up to the international standard in terms of equipments and techniques by employing modern engineering technology, which can maximally ensure the biological activity rotein in the food can be hydrolyzed into micro-molecule peptide or amino acid by bromelain, thus, bromelain can be made into tenderizer for meat, softener for biscuits and heal the food.

食品工业〕浇酶生产线达到国际水平的生命在用设备与技术近代工程技术可以最大限度保证生物活性蛋白的食物,可水解成微分子肽或氨基酸由菠萝,因此,菠萝可制成嫩肉肉、饼干、柔软剂为治好食物。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。