肽酶

In order to express he Opening Reading Frame fragment of Banana bunchy top virus Hainan isolateDNA2 correctly ,a pair of primers were designed in accordance with the reported BBTV-HN DNA2 gene sequence.

本文根据已报道的香蕉束顶病毒海南分离物(Banana bunchy top virus BBTV-HN)BBTV DNA2的基因序列，设计一对引物，以海南地区表现束顶病症状的香蕉组织总DNA为模板，利用PCR方法克隆了DNA2编码区，将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶基因下游。

OBJECTIVE: To review the recent advances in research of peptidyl-prolyl cis-trans isomerase Pin1 in tumor.

目的：总结肿瘤中肽基脯氨酰顺反异构酶Pin1的研究进展。

Cyclophilin A is a important member of a large family of ubiquitous peptidyl-prolyl cis-trans isomerases that regulate protein folding .

Cyclopnilin A是生物界普遍存在的肽基脯氨酸顺／反异构酶家族的主要成员，能调节细胞内蛋白质折叠。

Cyclophilin A is a important member of a large family of ubiquitous peptidyl-prolyl cis-trans isomerases...

Cyclopnilin A是生物界普遍存在的肽基脯氨酸顺／反异构酶家族的主要成员，能调节细胞内蛋白质折叠。

RESULTS: Peptidyl-prolyl cis-trans isomerase Pin1 specifically binds the motif of phosphorylation of Ser/Thr-Pro and regulates the function of phosphorylated proteins.

结果：肽基脯氨酰顺反异构酶Pin1能特异性地识别磷酸化的丝／苏－脯氨酰基序，调控磷酸化蛋白的功能。

Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.

应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。

Here we show that signal peptides have a new role beyond targeting: they are essential allosteric activators of the translocase.

在这里我们展示了信号肽除了靶向作用外的新的作用：他们是转移酶的重要的变构激活剂。

A role for signal peptides and targeting sequences as allosteric activators may be universal in protein translocases.

这种信号肽在对转移酶的变构激活中的作用可能是普遍的。

A total of 200 strains (z50% gentamicinresistant) from the United States were used for the cross-resistance study including Staphylococcus aureus (110), coagulase-negative staphylococci (CoNS; 50), Pseudomonas aeruginosa (10), Escherichia coli (20), and other Enterobacteriaceae (10) tested against TAO, bacitracin, polymyxin B, neomycin, amikacin, gentamicin, streptomycin, tobramycin, and mupirocin. Fifty gentamicin-resistant isolates from each year (1997–2002) were used to determine the activity of TAO over time. Baseline resistance rates of TAO among 300 Australian isolates (AGARS Program, 2002–2003) were also studied.

来至美国的200株菌株（ z50 ％对庆大霉素有耐药性）用于交叉耐药性研究，其中包括金黄色葡萄球菌（ 110株），凝固酶阴性葡萄球菌（ CoNS ； 50株），铜绿假单胞菌（ 10株），大肠埃希氏菌（ 20 株）和其他肠杆菌属菌（ 10株），检测这些菌对于以下抗生素的耐药性，抗生素为TAO，杆菌肽，多粘菌素B ，新霉素，丁胺卡那霉素，庆大霉素，链霉素，妥布霉素，莫匹罗星。1997到2002年期间每年分离出五十株对庆大霉素有抗药性菌株来确定随时间的变化TAO对其的活性是否有所改变。300株澳大利亚分离的菌株(AGARS Program， 2002–2003)也对TAO的耐药性基准进行了研究。

After the rats were orally administered with 0.066 4 g/, 0.134 g/, 0.4 g/ lycopene and the oil for 30 days, the concents of serum MDA, the energy of serum SA, the energy of serum GSH-Px and the contents of lipofasciin in liver were observed.

各组大鼠分别经口灌胃给予0.066 4 g/？0.134 0g/？0.400 0 g/的番茄红素及食用油30天后，测定血清中MDA含量？超氧化物歧化酶活力？谷胱甘肽过氧化物酶活力？

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。