肽酶

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

This study was aimed to ascertain the relationship between the distribution of glutathione S-transferaseM1 and T1 gene polymorphism related to the ris k of bladder cancer development and the medical surveillance for the bladder cancer high risk population exposed to benzidine.

研究旨在探讨与膀胱癌发生危险有关的谷胱甘肽S-转移酶M1和T1基因型的多态性的分布与有联苯胺接触的膀胱癌高危人群医学监护结果的关系。

This thesis mainly focus on the Enzyme histochemistry and TEM observation of trunk muscle from Branchiostoma belcheri tsingtauense; isolation and recombinant expression of Bbt-myosin heavy chain isoforms; characterization and expression of Bbt-nascent-polypeptide-associated complexαsubunit.In NADH Enzyme histochemistry, two types of muscle lamella can be distinguished .

本论文主要对青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌肉组织进行了酶组织化学实验和电镜观察，分离了青岛文昌鱼肌球蛋白重链基因异构体并将其重组表达；克隆了青岛文昌鱼新生肽链相关复合体α亚基基因，并检测了其时空表达情况。

The tryptophan residues play important roles in DNA photoreactivation catalyzed by CPD photolyases. We have investigated the action mechanism concerning Trp in DNA photoreactivation, and obtained the results as follows: 1 We proposed a mechanism of photosensitized splitting of CPDs by Trp and Trp-containing peptides. 2 Quantum yields of splitting of the model compounds that a Trp linked covalently a CPD were obtained under 290 nm light in various solvents, these values changing with solvent, range from 0.01 to 0.5. 3 Utilizing pulse radiolysis technique, splitting processes were observed in aqueous solution of the CPDs with hydrated electron and some oxidative species (such as hydroxyl radical, bromic radical and azide radical), and obtained their rate constants. In the case of interaction between CPD and hydrated electron, splitting of the CPD anion, and pyrimidine anion transfer an electron to FAD or Rf, were observed. The process rate was slower one order more than that in photolyase. Minor, but highly mutagenic UV-light-induced lesions are (6-4) photoproducts, which are repaired by (6-4) photolyases. These enzymes, discovered in 1993, are not well characterized and their mechanism is still under investigation.

我们研究它的作用机理，获得以下结果：1）建立了色氨酸及其肽对嘧啶二聚体光敏化裂解的直接修复作用机理模型；2）获得了色氨酸共价键连接于嘧啶二聚体的化合物在多种溶剂中290nm光辐照裂解的量子效率，其值随溶剂而变化，范围在0.01-0.5；3）运用脉冲辐解观察到嘧啶二聚体与水合电子和一些氧化性的自由基作用裂解过程，得到相应速率常数；对二聚体与水合电子作用发生裂解以及向FAD返还电子的过程进行了体外模拟；4）（6-4）光解酶的作用机理中一个关键假定是氧杂环丁烷中间体在接受电子后能自发裂解，恢复二嘧啶的结构。

The corn protein flour was hydrolyzed by a fractional hydrolysis with alkaline protease and caroid to produce corn peptide flour and the hydrolysis technological process was determined.

利用碱性蛋白酶和木瓜蛋白酶双酶对玉米蛋白粉进行分步水解，确定制备玉米肽干粉的工艺流程。

This enzyme catalyses the conversion of angiotensin I to angiotensin II in the blood plasma and endothelia and prevents the breakdown of bradykinin.

这种酶催化的血管紧张素一血管紧张素血液中的血浆和内皮二，防止故障的激肽转换。

Methods; In APS treatment group and control group, levels of insulin, C-peptide, myocardial enzymes, glycosylated serum protein and angiotensin were measured; Expression levels of chymase and angiotensin-coverting enzyme mRNA were detected by RT-PCR.

检测APS治疗组和对照组DM仓鼠的胰岛素、C肽、心肌酶谱、糖化血清蛋白和血浆。

Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen Ⅰ and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group, while there was no diference in ACE mRNA expression and activity between the two groups.

结果 APS组血糖、糖化血清蛋白、心肌酶谱和心肌Ang Ⅱ水平较DM组显著下降，胰岛素、C肽、血浆AngⅡ水平和DM组无差异；APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降；APS组chymase mRNA表达和活性均显著低于DM组，ACE基因的表达和活性与DM组无显著性差异。

While levels of insulin. C-peptide and plasma Ang Ⅱ were similar. APS group showed lower levels of chymase mRNA expression and activity than those of DM control group, while there was no diference in ACE mRNA expression and activity between two groups. Compared with DM group, expression level of p-ERK1/2 in APS group was much lower.

结果：APS治疗组GSP、心肌酶谱和心肌AngⅡ水平较对照组显著下降；血胰岛素、C肽、血浆AngⅡ水平与对照组无显著差异；APS组chymase mRNA表达和活性均显著低于对照组，ACE基因的表达和活性与对照组无显著差异；APS组心肌p-ERK1/2含量较对照组明显降低。

Methods In APS group and diabetes mellitus control group, levels of serum glucose, insulin, C peptide, myocardial enzymes, glycosylated serum protein and angiotensin Ⅱ in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme mRNA were detected by using fluorescent quantitative polymerize-chain-reaction; Activities of chymase and ACE were tested by radioimmunoassay.

检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平，血浆和心肌组织的AngⅡ含量；免疫组化法检测心肌Ⅰ、Ⅲ型胶原；荧光定量PCR法检测心肌chymase和ACE mRNA表达；放免法检测chymase和ACE活性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。