肽酶

Objective: To investigate the expression and their correlation of P-gp,GST-π,TopoⅡ and p53 in breast carcinoma.

目的：探讨乳腺癌中P-糖蛋白，谷胱甘肽-S-转移酶，拓扑异构酶和P53的表达及相关性。

This paper summarizes the research progress on the family of antioxidant enzymes in trematodes including glutathione peroxidase, superoxide dismutase and peroxiredoxin in the past decades.

本文综述近年来对医学吸虫谷胱甘肽过氧化物酶（glutathione peroxidase，GPx）、超氧化物歧化酶（superoxide dismutase，SOD）及抗氧化蛋白（peroxire-doxin，PRx）等抗氧化酶家族的研究进展。

Objective: To investigate the inhibitory effect of a new highly se lective inducible nitric oxide synthase inhibitorNGnitroargininelysine tripeptide on cNOS and iNOS indu ction .

目的：研究硝基精氨酸赖氨酸三肽对组成型一氧化氮合酶和诱导型一氧化氮合酶的抑制程度。

The activities of catalase,glutathione peroxidase,superoxide dismutase and protein contents in renal homogenates of rats exposed to TNT and treated with clofibrate were observed at present study.

内容摘要本研究观察了三硝基甲苯不同剂量染毒和阳性对照安妥明处理对大鼠肾组织匀浆过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶活性及肾组织蛋白含量的影响。

Some elements of proteins,such as architectures, catalytic site of enzymes, coenzymes, cofactors, and short sequences and so on, is very conserved during evolution that can serve as molecular fossils to help infer the characters of primitive proteins on structures and functions.

蛋白质的某些性质，包括折叠类型、酶的催化位点、辅酶、辅因子以及部分短肽片段等，在进化过程中是非常保守的。

The susceptibilities of the 3th instar larvae of Spodoptera litura fed on diets treated with nicotine and rutin to AucaNPV, fipronil and spinosad were studied. The activities of CarE and GSTs in different treated cutworms were also tested. The results showed that the nicotine enhanced significantly the susceptibility of Spodoptera litura to insecticides, and the susceptibility enhanced as the nicotine concentration in the diets increased.

以在人工饲料中添加植物次生物质的方法，研究了取食不同含量烟碱、芸香苷的斜纹夜蛾Spodoptera litura 3龄幼虫对银纹夜蛾核多角体病毒、氟虫腈及多杀菌素的敏感性，同时测定了各处理组幼虫体内羧酸酯酶和谷胱甘肽转移酶的活性。

The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

Thiols are also active in the catalytic sites of enzymes,and play important roles in metabolic pathways.

细胞内巯基化合物(包括半胱氨酸、高半胱氨酸和还原型谷胱甘肽等)在维持生命体系的氧化还原动态平衡方面起着重要的作用，它们是与酶活性有关的最具有反应性的官能团，并且在酶代谢过程中起着重要的作用。

Larval white leg shrimp Litopenaeus vannamei were fed steamed egg custard with 0.005%, 0.01% and 0.1% peptidoglycan, and immersed in the seawater containing 0.005, 0.01 and 0.05 mg/ml PG, and fed live food as control group. Acid phosphatase and alkaline phosphatase activities were tested after postlarvae (PL1) were fed for 7 days.

用含不同质量分数(0.005%、0.01%、0.1%)A3α肽聚糖的饵料投喂及在育苗水体中添加不同质量浓度（0.005、0.01和0.05 mg/ml）的PG浸浴凡纳滨对虾幼体，以投喂不添加PG的饵料作对照，另设投喂活饵的试验组作参考；饲养7 d后，检测PL1期仔虾体内酸性磷酸酶和碱性磷酸酶活性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。