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Ribose/galactose/methyl galactoside importing ATP-binding protein 1 was participated in photosynthates transportation from leaf to grain in early and mid-stages.

分析结果表明,核糖/半乳糖/甲基半乳糖苷运输ATP结合蛋白l在灌浆前中期参与物质向籽粒的运输;生长素响应蛋内IAA27通过调节ATPase活性影响叶片物质运输;N-乙酰谷氨酸半醛脱氢酶在灌浆末期参与叶片的多胺代谢,延缓叶片衰老;谷胱甘肽S-转移酶和Cu/Zn-超氧化物歧化酶在籽粒灌浆后期的植物解毒和防御活性氧伤害中起着重要作用。

The method uses CCl4 revulsive small rat liver injures a model, medicaments is preventive experiment, through determining transaminase of serum cereal third , millet straw and liver organization third 2 aldehyde content, yellow purine oxidizes peptide of pleasant of Guang of enzymatic, cereal is reductive enzymatic active and liver tectology change are index, observation grackle extracts the protective effect that content injures to liver.

方法采用CCl4诱导小鼠肝损伤模型,药物预防性实验,通过测定血清谷丙转氨酶、谷草转氨酶和肝组织丙二醛含量、黄嘌呤氧化酶、谷胱甘肽还原酶活性以及肝组织形态学变化为指标,观察白头翁提取物对肝损伤的保护功能。

The complex of flavourzyme and neutral protease was used to hydrolyze camellia pollen to obtain bioactive peptides.

茶花花粉蛋白经过风味酶和中性蛋白酶组成的复合酶水解得到活性肽。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

In currence, se- GSH- PX activity have been been used as a standard of Selenium nourishment, GSH- PX is an extremely important protection enzyme in anti oxida- tive defend system of organize and cell of animal.It clear hydroxy radical and hyperoxide in cell respir- ation metabolism reaction ,thereby, mai- ntain cell's normal physiological function, prote- ct biomembrane and biomacromolecule from oxidative damage.If the activity is high or not, effect the ab- ility that the organize clears hyperoxize lipi- d ,directly.

目前,含se-GSH-PX活性已被作为se营养状况可一个指标,该酶是动物组织细胞抗氧化防御系统中一个极为重要的保护性酶,它是以还原型GSH为底物的谷胱甘肽参与的过氧化反应,清除细胞呼吸代谢反应过程产生的羟自由基和过氧化物,从而使细胞能维持正常的生理功能,保护生物膜和生物大分子结构免受氧化损伤。

Phosphoglycerate kinase 1, fumarate hydratase and aldolase A were expressed in primary cancer.

精氨酸酶,谷胱甘肽S-转移酶A3在肝转移灶中表达上调。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因,根据鸡VIP基因(GenBank登录号X80906),设计了一对简并引物,从北京鸭腺胃、十二指肠和空肠提取总RNA,通过反转录-聚合酶链反应扩增,将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上,导入大肠杆菌JM109,阳性克隆经双酶切鉴定后测序,将测序结果与鸡和鹅(GenBank登录号为DQ023161)的VIP基因进行同源性比较。

Laboratory diagnostic markers of acute pancreatitis in children include amylase and Isozyme,lipase,c-reactive protein,urinary trypsinogen2,trypsinogen activation peptide,procalcitonin and so on.

小儿急性胰腺炎的实验室诊断标志物包括淀粉酶及同工酶、脂肪酶、C反应蛋白、尿胰蛋白酶原2、胰蛋白酶原激活肽、降钙素原等。

Superior mesenteric artery was clipped for 60 min to cause ischimia, and then unclipped to make reperfusion for 2 hours after reperfusion, the liver tissues were gathered and homogenized respectively, and the contents of SOD, GSHPX, MDA and ATPase,and morphological changes of hepatocytes were assayed.

夹闭大鼠肠系膜上动脉60 min造成缺血,再灌注2 h后取出肝组织制成匀浆,测定超氧化物歧化酶、谷胱甘肽过氧化酶、丙二醛、Ca2+Mg2+ATP酶的含量及肝形态细胞学变化。

80 Mg/L on Paralichthys olivaceus antioxidant system in muscle,gill,viscus,by using the activities of CAT,SOD and GSH-PX as indexes.

以鳃、肌肉和内脏组织超氧化物歧化酶SOD、过氧化氢酶CAT、谷胱甘肽过氧化物酶GSH-PX活性为指标研究了Cu2+污染对牙鲆的损伤作用。

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