肽酶

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

It was also demonstrated the inhibitors could inhibit the activities of enzymes to a great degree, the bacitracin and casein showed the prominent effect.

同时也证明了加入酶抑制剂能够对rHV2提供很大程度的保护，其中杆菌肽和酪蛋白的酶抑制作用比较突出。

The aim of this work was to study the effect of Cu2+ on the activities of antioxidant enzyme CAT,SOD and GSH-PX in the Gill of Chlamys farreri .

将栉孔扇贝置于不同浓度的Cu2+溶液中10d，测定其鳃组织过氧化氢酶，谷胱甘肽过氧化物酶，超氧化物歧化酶的活性。

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

And the two complexes have been found to be single-strand DNA cleavers in the presence of glutathione/O〓 or ascorbate/O〓 and DNA-nicking efficiencies of two complexes are ＜.

这两个配合物在抗坏血酸/O〓或谷胱甘肽/O〓的激活下均呈现化学核酸酶活性，它们的核酸酶活性大小为：＜。

The facts that type I compounds could easily undergo deamination reaction to form α-methylene cyclopentanone derivatives and they could react with glutathione to form an adduct provided sufficient evidence for the hypothesis that the compounds acted as masked α-methylenecyclopen- tanones and reacted with biologically important sulfhydry-containing enzymes, and thus produced anticancer activity.

Ⅰ类化合物在稀溶液中极易进行去胺基化反应形成α-亚甲基环戊酮类化合物。并且Ⅰ类化合物于稀溶液中易与谷胱苷肽进行结合，这些事实为Ⅰ类化合物作为隐蔽的α-亚甲基环戊酮与酶进行加成，从而导致酶失活而具有抗癌活性的设想提供了证据。

In our experiments, when the opacity of lens exceed Ⅳ degrees, the treatment effect of medicine was not obviously. Analyzing the examined results of AR, SOD and GSH-px in rat lens cortex and nuclear shows that Zhangming drops can inhibit the activity of AR obviously and prevent the translation of galactose to galactose alcohol. So it can prevent the deposit of galactose in the fibrocyte of lens and the increase of lens's osmosis, then prevent the hydration of lens.

通过对大鼠半乳糖性白内障晶体皮质和核内醛糖还原酶、超氧化物歧化酶和谷胱苷肽过氧化物酶的检测结果分析表明，障明滴眼液能明显抑制AR的活性，防止半乳糖向半乳糖醇的转化，从而防止半乳糖在晶体纤维细胞内的堆积，阻止其渗透性的增高，防止晶体的水化。

Screening ofprotease pathways led the researchers to implicatekallikrein–kinin activation in this process.

通过对蛋白酶通路的筛选，研究人员发现激肽释放酶-激肽的激活参与了这个过程。

Keywords: NMT; Randomly phage display library; Affinity binding; Screening of inhibitor peptides; Molecule-screening model

中文关键词：豆蔻酰转移酶；噬菌体展示随机肽库；亲和结合；抑制肽筛选；分子筛选模型

Ten of them were new sequences and the other 7 were duplicated sequences. These sequences represented respectively: depeptide ABC transporter periplasmic dipeptide-binding protein, permease protein, et al. Conclusion, Gene fragments specific to MTZ-resistant H.

对以上17个差异片段进行单向测序，根据最高同源性比对结果，分别代表二肽ABC载体渗透酶、二肽ABC载体结合蛋白等10个基因的同源基因片段在耐药株中存在高拷贝数。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。