肽酶

Using the middle cerebral artery occlusion modelin rats and cerebral ischemia-reperfusion models in gerbils and mice, we investigated the influence of pueraria compound on the brain water content and the infarct size, the cerebral apoplexy exponent, the contents of lactic acidand lipid peroxide, the activities of lactic dehydrogenase, glutathione peroxidaseand Na+-K+-ATPase.

采用大鼠大脑中动脉栓塞模型、沙土鼠全脑缺血再灌模型和小鼠脑缺血再灌3种动物模型，研究复方葛根素对脑缺血再灌注脑含水量和梗塞范围、脑卒中指数和脑组织中乳酸、乳酸脱氢酶、过氧化脂质、谷胱甘肽过氧化物酶、Na+-K+-ATP酶的影响。

Aegle marmelos ; Anti-lipid peroxidation ; 4-Methoxy-1-methyl-2-quinolone; Superoxide dismutase; Catalase ; GSH-peroxi

木桔；抗脂质过氧化作用； 4－甲氧基－1－甲基－2－喹诺酮；超氧化物岐化酶；过氧化氢酶；谷胱甘肽过氧化物酶

According to the condition of the dispase and alkaline proteinase, we combine the two enzymes so as to get the most optimization method to improve the yield of dissolvable peptide.

通过对单酶水解条件的摸索，将选择的两种酶以适当的比例混合，并且通过控制反应条件，提高蛋清蛋白的水解度和可溶性肽的得率，从而研制出一种适合蛋清白蛋白水解的复合酶。

The six stages involved euthermic state (25℃), prehibernation (10-12℃), after hibernating for a week and a month (2-4℃), and arousing from hibernation for a week and two weeks (10-12℃). From euthermic state to long-term hibernation, lipid peroxidation was showed to increase in brain except the stage of prehibernation.

分别测试了上述各阶段荒漠沙蜥1％脑组织匀浆、0.5％心脏组织匀浆及1％肝脏组织匀浆中：丙二醛及过氧化氢(H_2O_2)的含量，超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性，及总抗氧化能力的水平。

METHODS: D-galactose (1 000 mg·k-1·d-1 ) was injected into mice hypodermically for 8 weeks to induce aging animal model, and Vit-E (100 mg·kg-1; 250 mg·kg-1) was administered for 6 weeks by ig at the 3rd week of making model. After Vit-E treatment for 8 weeks, water maze test was used to determine the ability of mice's learning and memory. The activities of glutathione peroxidase and succinate dehydrogenase, the content of nitric oxide and activity of nitric oxide synthase in the brain tissue were detected separately.

小鼠连续皮下注射D-半乳糖（1 000 mg·k-1·d-1）8周制备衰老模型，并于第3周开始给予维生素E（100 mg· kg-1；250 mg· kg-1）处理；8周后采用水迷宫测定小鼠学习记忆能力，并取脑组织测定谷胱甘肽过氧化物酶和琥珀酸脱氢酶活性，测定一氧化氮含量和一氧化氮合酶活性。

The free radical scavenging effects of Meretrix meretrix hydrolysates prepared with papain,neutral protease and acid protease were investigated.

利用正交实验研究了文蛤蛋白分别经木瓜蛋白酶、中性蛋白酶、酸性蛋白酶作用后酶解物对羟自由基和超氧自由基的清除作用，优化了水解条件，并对最佳清除率下的三种酶解物进行SephadexG50凝胶柱分离，测定酶解物中抗氧化活性肽的分子量分布。

The resistance mechanism of wheat to insects includes tolerance, nonpreference and antibiosis. The dissertation investigates the wheat mechanism, indicators and standard of resistance to aphid and analyses the relation between four kinds of second substance and the wheat resistance to aphid and research the effect of wheat varieties on carboxylesterase and glutathione-s-transferase according to the tests of three mechanism, the content of second substance and the activity of relative detoxification enzyme.

本文通过不同小麦品种的不选择性、抗生性、次生物质含量及麦长管蚜体内有关解毒酶活性的测定，进一步探讨了小麦的抗蚜机制，探索了小麦的抗蚜指标及鉴定标准；研究分析了小麦中4种重要的次生物质含量与小麦抗蚜性的关系；测定了不同小麦品种对麦长管蚜羧酸酯酶和谷胱苷肽-S-转移酶的影响。

After gestational periods the activities of SOD,MDA,GSH-Px,TChE and CAT in brain were analyzed respectively.

采用饮水染毒，染毒时间为21d，分娩后每窝随机选取6只乳鼠测定脑匀浆超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、乙酰胆碱酯酶活性和丙二醛的含量。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

In our results, we found four proteins have isoforms; these proteins are peptidyl-prolyl cis-trans isomerase A, prohibitin, enolase 1, myeloperoxidase precursor.

同时，我们发现肽基辅胺酸顺反异构酶（peptidyl-prolyl cis-trans isomerase A）、抑制素、烯醇酶（enolase 1）、过氧化骨髓酶有异构体的产生。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。