肽酶

The optimal pH of xylanases was calculated based on the model and the results was shown as below: there were only two xylanses with the deviation between the calculated pHs the optimum pHs larger than 0.6, while there 6 xylanases with the deviation less than 0.1 the mean absolute percent error was 5.91%, the mean absolute error were 0.26 pH unit, respectively. It was superior when compared with the reported stepwise regression model based on dipeptide composition.

利用该方程，计算各木聚糖酶的pH值，结果表明，仅有2个木聚糖酶的最适合pH计算值与实测值相差大于0.6，而有6个木聚糖酶最适pH计算值与测定值相差小于0.1，平均绝对百分比误差为5.91%，平均绝对误差为0.26个pH单位，所得结果优于用二肽进行逐步回归所得模型的结果。

After transfection with human carbonic anhydrase Ⅱ cDNA, the lovo cells changed obviously with reduction in invasiveness,chemotaxy motor ability and tolerance. Conclusion Differential expression of proteins was found between colorectal cancer genesis and hepatic metastasisnegion.

结论结直肠癌原发灶和肝转移灶蛋白质组表达有显著差异，碳酸酐酶Ⅱ表达下调和精氨酸酶、谷胱甘肽S-转移酶A3增强表达可使结直肠癌细胞生物学行为发生改变并促进肝转移发生。

Effects of water stress on the level of reactive oxygen speciesand activities of antioxidant enzymes in the leaves of wheat(Triticum durum Desf.) seedlings were investigated.Water stress resulted in significant increase in the contents of O-.2,MDA and the activities of superoxide dismutase and ascorbate peroxidase in wheat leaves.However,the activities of glutathione reductase,catalase and the content of H2O2 were not changed obviously under water stress.

分析了水分胁迫对小麦幼苗叶片中活性氧水平和抗氧化酶活性的影响，结果发现，水分胁迫能够显著提高超氧阴离子(O-2·)和丙二醛的含量及超氧化物歧化酶和抗坏血酸过氧化物酶的活性，而过氧化氢(H2O2)的含量及过氧化氢酶和谷胱甘肽还原酶的活性变化不明显。

The result of acute toxicity of Cd(superscript 2+) on antioxidant enzymes showed that along with the rise of concentration of Cd(superscript 2+), the activity of superoxide dismutase, catalase, and guaiacol peroxidase were changed in O.chinensis injected with Cd(superscript 2+).

对Cd(上标 2+)急性染毒的中华稻蝗抗氧化酶活性变化研究表明，随着Cd(上标 2+)浓度的升高，超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性均发生改变。

Taking the ratio of holothurian peptides as an index,the effect of the added amount of trypsinase ,time,pH,temperature and substrate concentration on the enzymolysis of holothurian were studied.

以海参肽得率为指标，分别考察了酶解加酶量、时间、pH、温度、底物浓度对胰蛋白酶酶解海参蛋白质反应的影响。

The activities of glutathione peroxidase, superoxide dismutase, catalase, the content of malonaldehyde in serum and lens were detected. At the same time, the activities of Na+-K+-ATPase, the content of macromolecular weight protein and infusibility protein in lens were detected too.

比色法测定各组血清及晶体中超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶活力和丙二醛含量以及晶体中Na+-K+-ATP酶活力、高分子质量蛋白和不溶性蛋白含量。

The Chitinase of Saccharomyces cerevisiae(CTS1-2) is an Endochitinase which is endogenous in Saccharomyces cerevisiae. Its recognition and slipt site lies in the β-1,4 glucosidic linkage of homopolymer. Included in the amino acid sequence from N-terminal to C-terminal of the Saccharomyces cerevisiae chitinase are signal peptide sequence, catalytic domain, binding domain and a C-terminal shorten region.

酿酒酵母几丁质酶(Chitinase，CTS1-2)是一种来自酿酒酵母的内切几丁质酶，它的酶切位点位于其同聚物内的β-1，4连接的糖苷键，酶蛋白分子结构从N端到C端依次为信号肽、几丁质催化区、几丁质结合区以及一个短的C末端区。

The effects of the food additive butylated hydroxyanisole and the antibiotic Norfloxacin on swordtail fish were measured by considering a number of parameters, including glutathione S\|transferase, catalase, 7-ethoxyresorufin-o-dealkylases and malondialdehyde contents.

研究了叔丁基对羟基茴香醚(butylated hydroxyanisole， BHA)和诺氟沙星(norfloxacin， NFLX)对剑尾鱼的急性毒性及其肝脏谷胱甘肽硫转移酶、丙二醛、过氧化氢酶及7-乙氧基异吩恶唑酮-脱乙基酶活性的影响。

MMPs are usually secreted as latent, inactive proenzymes, which need to be activated (Fig 1 ), and/or are bound to their native inhibitors, the tissue inhibitors of metalloproteinases.8 Activation may involve proteolysis and/or oxidation of the latent enzyme.8 Proteolytic activation of the proenzymes cleaves the propeptide sequence, which masks the active site of the latent enzyme.

金属蛋白酶常以无活性的酶原方式分泌，而后者要么需要被激活要么与附近的或组织中金属蛋白酶抑制物结合。激活作用包括了具有潜在酶的蛋白水解作用或氧化作用。酶原的蛋白水解激活作用能将掩盖活性部位的潜在酶的前肽序列破坏。

Methods Male SD rats were given drinking water containing manganese chloride (MnCl24H2O 0.5g/L and 5.0g/L respectively) for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, cholinestarase, monoamine oxidase, superoxide dismutase, nitric oxide synthase, and levels of glutathione, maleic dialdehyde, and proteins were biochemically analyzed.

大鼠经饮水连续染锰（MnCl24H2O 0.5g/L和5.0g/L）60d后，应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶、谷草转氨酶、碱性磷酸酶、胆碱酯酶、单胺氧化酶、氧化亚氮合酶、超氧化物歧化酶活性和谷胱甘肽、丙二醛、蛋白质含量，并设生理盐水对照组。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。