肽酶

Analogues of GLP-1 and Dipeptidy peptidase-Ⅳ inhibitors have a better prospect in the recent study.

其可被二肽基肽酶Ⅳ降解，半衰期极短，限制了其临床应用，目前研究GLP-1类似物及二肽基肽酶Ⅳ抑制剂有更好的应用前景。

Using of matrix assisted laser desorption/ionization time of flight mass spectrometry coupled with carboxypeptidase Y digestion for the analysis of C terminal peptide ladder by direct determination of the mixture of truncated peptide, the sequence of C terminus can be read out simplely from the mass differences between adjacent peptide peaks on the mass spectrum.

用羧肽酶Y水解蛋白质和多肽，结合飞行时间质谱测定酶解后缩短肽的质量，根据相邻两肽峰的质量差别读出蛋白质和多肽的C端序列，在pmol水平下测定了人促肾上腺皮质激素片段以及人血管紧张肽片段，其中人促肾上腺皮质激素片段测到了C端 15个氨基酸残基的顺序

The fact that the three additives and TP5 were with opposite charges in solution and therefore attracted each other electronically to form an ionic complex assumably explained the mechanism of their enzyme inhibitions.

经分析可能有两种肺酶参与TP5水解：中性内肽酶从肽链的C末端水解掉两个氨基酸残基，氨肽酶从肽链N末端水解掉精氨酸。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

ACE is also known as kininase II, part of the kallikrein-kinin- prostaglandin system. As kininase II, this enzyme is involved in the breakdown of kinins, which are potent vasodilators.

ACE也称为激肽酶II，是激肽释放酶-激肽-前列腺素系统的一部分，激肽是一种强有力的血管扩张因子，激肽酶II介导激肽降解。

Characterization of senescence-associated endopeptidase iso -zymes in wheat leaves The characterization of senescence-associated endopeptidase isozymes in wheat leaves during dark-induced senescence was performed.

而采用将明胶为底物的SDS-PAGE方法只能检测到一种新内肽酶同工酶的出现，因此衰老期间表达的内肽酶同工酶大都是对SDS敏感的或多亚基的蛋白水解酶。

The presence and absence of γ-glutamyl transpeptidases in sprouting and dormant garlic bulbs were determined.The relation between the activity of γ-glutamyl transpeptidases in the garlic and greening of garlic purees was investigated.

通过测定休眠与打破休眠的大蒜中γ-谷氨酰转肽酶的活性存在与否，大蒜中γ-谷氨酰转肽酶活性的变化与蒜泥绿变的关系，以及在不能绿变的大蒜中添加γ-谷氨酰转肽酶而使蒜泥变绿，确定了γ-谷氨酰转肽酶对蒜泥绿变的作用。

The total activity of EPs increased at first and then decreased during both senescences. The changes of endopeptidase isozymes in wheat leaf senescence were studied by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with hemoglobin or gelatin in the gel.

主要研究内容如下： 1、小麦叶片衰老过程中内肽酶的变化蛋白质的降解需要蛋白水解酶，包括内肽酶和外肽酶的参与，叶衰老过程中发挥主要作用的是内肽酶。

It suggested that the AEBSF delayed leaf senescence though inhibition the endopeptidase activity and influence the SOD and POD activity.Native PAGE with gelatin as substance in gel showed the activities of isoenzyme 3 and 4 were inhibited partly by AEBSF treatment on leaves.It implies that the two isoenzymes are type of serine endopeptidase and may play an important role in cucumber leaves senescence. Degradation of protein and changes of endopeptidase isoenzymes in cucumber Cucumis sativus L.

活性电泳显示，黄瓜叶片中检测到6条内肽酶同工酶，其中4条(CEP2，3，4，6)为丝氨酸型内肽酶，内肽酶抑制剂AEBSF抑制了叶片中丝氨酸内肽酶同工酶的活性，而ABA使丝氨酸内肽酶CEP2，3，4的活性显著增强，再次暗示了丝氨酸型内肽酶在黄瓜叶片衰老以及叶片Rubisco降解中具有重要作用，而且除了丝氨酸内肽酶外，可能还有其它因素参与了衰老的调控。

Six senescence-associated endopeptidase isoenzymes were identified by using natural gradient-polyacrylamide gel electrophoresis co-polymerized with gelatin in the gel, five of which (EP1、EP2, EP4、EP5 and EP6) were only detected in senescing leaves.

另外，采用不同的内肽酶同工酶电泳方法研究中均发现自然衰老和暗诱导衰老过程中的内肽酶同工酶谱变化基本相似，因此暗诱导衰老可以作为一种研究叶片自然衰老过程中出现的蛋白水解酶的特性的模式。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。