肽合成酶

The Culture of Green beaus in extract solution of seven poisonous mushrooms resulted in different effects. There are obvious difference among the content of the proteins in Green beaus which were cultured in extract solution of seven poisonous mushrooms, and it is thought that α-amanitin in extract solution of these mushrooms can inhibit the function of RNA polymerase Ⅱ in eukaryoctic cells and further inhibit the synthsis of protein.

用鹅膏菌属含α-毒伞肽的毒素粗提液培养新鲜绿豆，结果在36小时后，各种不同毒苗的毒素粗提液培养的绿豆生长情况有明显不同，用紫外吸收法测定蛋白质含量，发现毒素粗提液培养的绿豆细胞中蛋白质含量比蒸馏水培养的有明显下降，这表明α-毒伞肽的作用机理的确是通过抑制RNA聚合酶Ⅱ而寻致蛋白质合成减少。

Most of these peptides are amidated at their C-terminals, which is essential for their full activities. The a-amide comes from posttranslational modification that is catalyzed by α-amidase (α-AE or PAM). The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides.

大部分活性肽的C-端是酰胺化的，而且α-酰胺基为多肽的完全生物活性所必需；α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶催化完成，是酰胺化多肽生物合成途径中的限述步骤。

In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分，我们按照交替氧化酶(Alternative Oxidase，AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列，用固相法合成由12个氨基酸残基组成的多肽，将此多肽与α-牛胰凝乳蛋白酶原A相连，用作半抗原，免疫家兔制备人工合成12肽的抗体。

The mechanism might be related to the degression of glucocorticoids and the activation of glycogen synthase.

肌肽增强应激负荷小鼠肝糖原合成能力，其作用机制可能与肌肽降低体内糖皮质激素水平、激活糖原合酶有关。

To detect the marks reflecting the liver functions as follows:①The marks to reflect the capability to synthesize proteins, TPROT, ALB, GLB;②The marks to reflect the injury of the liver cells: AST, ALT, CHE;③The marks to reflect the capability to egest the bile, excrect, and detoxicate: TBILI, DBILI, IBILI, TBA;④The enzymes to reflect the cholestasis: GGT, ALP. Results The results of both the morphological and the pathological studies showed that the degree of the liver cirrhosis was of the terminal stage.

肝功能指标：①反映肝细胞蛋白合成代谢功能的指标：总蛋白TPROT，白蛋白ALB，球蛋白GLB；②肝细胞受损的指标；谷草转氨酶AST，谷丙转氨酶ALT，胆碱脂酶CHE；③反映肝脏胆排泄、分泌及解毒功能的指标：总胆红素TBILI，直接胆红素DBILI，间接胆红素IBILI，总胆汁酸TBA；④胆汁淤积指示酶：谷氨酰转肽酶GGT，碱性磷酸酶ALP。

Aim To find novel dipeptidyl peptidase IV inhibitors with thiazole rings and to test their anti-diabetic activities.

摘要目的合成一类新型的以噻唑环为母体的二肽基肽酶IV抑制剂，并测试它们在治疗糖尿病方面的活性。

Sixteen thiazole-bearing compounds were designed and synthesized as dipeptidyl peptidase IV inhibitors, and characterized by IR, 1H NMR, 13C NMR and HR-MS techniques.

设计并合成了16个含噻唑环的二肽基肽酶IV抑制剂，利用IR， 1H NMR， 13C NMR和HR-MS表征了它们的结构。

Cefaclor is a kind of cephalosporin antibiotics which is extensively used for anti-infection. It will devitalize transpeptidase, disturb the final step synthesis of the cell wall and prevent the cross bonding of mucopeptide.

头孢克洛是广泛应用于抗感染的头孢菌素类抗生素药物，它的杀菌作用是使转肽酶失活，干扰细菌细胞壁最终阶段的合成，阻止粘肽原的交联。

Freezing reaction mixtures as a new approach for kinetically controlled peptide synthesis, however, was developed by Jakubke et al.

但低温酶促肽键合成作为一种新的肽键生成方法则在1990年Jakubke等人的报道之后才开始受到关注。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。