肽

Up to now, there is no such report about the study of tussah silk peptide products, neither inland nor overseas.

目前，无论国内还是国际，有关柞蚕丝肽产品的研究均是空白，利用柞蚕丝肽研制柞蚕丝肽化妆品，具有重要的理论意义及现实意义。

Angiotensin-converting enzyme also breaks down BRADYKININ, a powerful vasodilator and component of the KALLIKREIN-KININ SYSTEM.

血管紧张素转换酶也分解缓激肽，一种有力的血管扩张剂和激肽释放酶-激肽系统的成分。

The effects of cryopreservation (-196℃) on the antioxidant activities of enzymes (SOD, CAT, GSH-PX and GR) in seminal plasma and spermatozoa of Russian sturgeon Acipenser gueldenstaedti were studied by a reagent kit to clarify damage mechanism of spermatozoa.

为了探讨超低温(-196℃)对精子的损伤机理，采用试剂盒测定了冷冻前后俄罗斯鲟精浆和精子中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷肽甘肽还原酶等酶活性。

According to the spetial conformational analysis and physical/chemical property analysis and by the aid of computer it can make molecular design of mimic polypeptide and designs the polypeptide molecule with activity for neutralizing LPS, and adopts the solid phase polypeptide chemical synthesis method to synthesize antibacterial/antiendotoxin mimic peptide with total or partial BPI bio-function.

本发明公开了通过计算机模拟设计、人工合成的66个抗菌／抗内毒素模拟肽，根据对BPIN端三个LOOP区的空间构象分析及物理化学性质分析，在计算机辅助下进行模拟多肽的分子设计，设计出具有中和LPS活性的多肽分子，用固相多肽化学合成方法合成抗菌／抗内毒素模拟肽，本发明的抗菌／抗内毒素模拟肽具有全部或部分BPI的生物功能，可以用于治疗G－细菌感染、人内毒素血症，具有分子量小、可以稳定重复生产的特点。

The blood samples were collected at day 1 before and after the caprine placenta peptide were given for 10 days, and the number of the white blood cell and the ANAE+ in periperal blood ,the content of the globulin, the activity of lysozyme, the T-Aoc ,the activity of the GSH-Px and SOD, and the content of the MDA in serum were determined .

在口服羊胎盘肽的前1d和连续口服羊胎盘肽10d后的第1d各组采血样一次，测定外周血白细胞数、ANAE+淋巴细胞百分率、血清球蛋白含量、血清溶菌酶活性、血清总抗氧化能力、血清谷胱甘肽过氧化物酶活性、血清超氧化物歧化酶活性及丙二醛含量。

The present study was designed to investigate whether histamine is involved in the protective effect of carnosine on Aβ42-induced impairment in differentiated PC12 cells.

因此本实验的主要目的是检测肌肽对Aβ42诱导的神经兴奋性损伤是否具有保护作用，肌肽-组胺酸-组胺通路是否参与其保护机制，谷氨酸含量，NMDA受体表达及功能在这一过程中是否受到影响，最终阐明肌肽保护作用的具体机制。

The experiments showed that polypeptides were formed by self-assembly of N, O- bis amino acids with the assistance of o-phenylene phosphoro- chloridate. Three steps were involved in the self organization reactions, i.

侧链无官能团的N，O-二-α-氨基酸在O，O-亚苯基磷酰氯辅助下能自组装得到系列2～n聚寡肽，其自组装成肽过程大致分为三步：氨基酸的活化、肽链的延长和链反应终止。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。