肽

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

RBL-2H3 cells expressing formyl peptide receptor being stimulated with two polypeptides was used as model system to characterize the chips' performance. As a result, fMLF triggered chemotaxis, receptor internalization and calcium mobilization, while GSH had no effect on the cells.

以含有不同氨基酸序列的多肽作用表达甲酰肽受体的RBL-2H3细胞为模型体系，对芯片组性能予以验证，结果表明，N-甲酰肽物质能够刺激细胞发生趋化运动、受体内吞和钙离子释放，而作为阴性对照的还原型谷胱甘肽无此能力。

To extract conformational motifs and construct fragmental libraries for peptide, it is important to analyze the structure of the conformational space of oligo-peptide.

研究蛋白质寡肽构象在构象空间中的分布情况，对提取寡肽模式并构建短肽库具有重要的意义。

A gray-brown mixture consisting mainly of tryocidine and gramicidin, used as a topical antibiotic in treating infections caused by gram-positive bacteria.

混合短杆菌肽主要由短杆菌酪肽与短杆菌肽构成的一种灰棕色混合物，主要用来制造治疗因革兰氏阳性细菌造成的感染的抗生素

A new analysis system for enrichment of glycoproteins/glycopeptides as well as the identification of glycoproteins/glycosylation sites was also established by coupling enrichment of glycopeptides by hydrazide- functionized magnetic microspheres with release of polysaccharide chains by PNGase F and mass spectrometry as well.

本实验还将肼功能化磁粒富集糖肽方法与糖肽酶酶解、生物质谱等方法相结合，建立起了一套可用于糖蛋白/糖肽分离富集并进行糖蛋白/糖基化位点鉴定的新体系。

Rats were killed after different weeks(0,1,2,4,8,12,15,17,19,20 and 24 respectively). Their organs were taken to observe the kinetic change of selenium concentration, the activities of intracellular glutathione peroxidase,extracellular glutathione peroxidase, and phospholipid hydroperoxide glutathione peroxidase in different organs.

于0、1、2、4、6、8、12、15、17、19、20和24周时处死大鼠取其组织，分别对各种组织中的硒、细胞内谷胱甘肽过氧化物酶、细胞外谷胱甘肽过氧化物酶、磷脂氢谷胱甘肽过氧化物酶、Ⅰ型脱碘酶和Ⅱ型脱碘酶活性进行动态观察。

Enrichment of phosphoproteins and phosphopeptides is a very critical step for MS analysis because of low abundance of phosphoprotein in cells and the suppressive effect from the nonphosphopeptides in protein digestion.

由于磷酸化蛋白在生物体内含量很低，且在质谱分析时易受到非磷酸肽的干扰，因而磷酸肽和磷酸化蛋白质的富集成为磷酸化肽和磷酸化蛋白质分析的一个关键步骤。

PACAP is a pleiotropic neuropeptide that belongs to the secretin/glucagon/vasoactive intestinal peptide family.

垂体腺苷酸环化酶激活肽是多效性的神经肽，属于肠促胰液素/胰高血糖素/肠血管活性肽家族。

Aureus secretor strain in a media containing vegetarian amino acids or peptides instead of animal peptides, harvesting the Protein A-containing media and purifying the Protein A by applying it to a synthetic resin that lacks animal peptides and filtering the elutant from the resin.

本发明涉及制备没有动物源产物污染的蛋白A的方法，其包括通过在含有植物氨基酸或肽代替动物肽的培养基中发酵金黄色葡萄球菌分泌菌株，收集含有蛋白A的培养基和纯化蛋白A，并且将其施加到没有动物肽的合成树脂上和过滤来自树脂的洗脱液。

Methods The antimicrobial peptide of M. domestica pupa were produced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method.

通过损伤加感染的方法诱导家蝇蛹大量表达抗菌肽，然后经过研磨、离心和AKTA purifier层析等步骤，将家蝇蛹抗菌肽进行分离纯化，采用血细胞计数法和MTT比色法筛选对弓形虫速殖子有抑制作用的抗菌肽。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。