肽

Full-length cDNA sequence of NPY from Barbodes sinensis Based on multiple alignment of a number of Cyprinidae NPY genes, two special primers of 3'cDNA ends were designed for amplification of the 3'cDNA ends of NPY genes and the sequence was acquired from the cDNA templet of the brain of S.sinensis.

中华倒刺鲃基因推导氨基酸序列的分析中华倒刺鲃NPY的cDNA编码区核苷酸长度为291bp，编码96个氨基酸，由信号肽(28个氨基酸)、NPY成熟肽(36个氨基酸)、三肽加工位点(3个氨基酸)和连接肽(29个氨基酸)组成。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

This article is a review on the mechanism about ruminant and monogastric animals, introduce the peptide transporters' molecular biological characters and its transport process, analyzed the factors that influence the small peptides' absorption.

本文综述了小肽在单胃和反刍动物体内的转运机制的特点，介绍了小肽转运载体的分子生物学特性和其转运肽的过程，分析了对小肽吸收的影响因素。

The content of essential amino acid and flavor amino acid of experiment groups were higher than control group, especially group 1.0% small peptide were increased by 6.4% and 2.5%; IMP content of experiment groups was increased in different extent, especially group 1.0% small peptide was 0.17mg/100mg and increased by 70.0%(P.05); The content of palmitoleic acid、 octadeca、 arachidonic、 arachidic and stearic acid of experiment groups were higher than control group indifferent extent.

随着小肽用量的增加，成鲤肌肉中的总氨基酸含量呈上升趋势，必须氨基酸和风味氨基酸的含量也随之提高，尤其是1.0％小肽组的必须氨基酸和风味氨基酸的含量分别比对照组提高6.4％和2.5％，其中谷氨酸的含量最高，为10.70mg／100mg，比对照组提高14.0％(P＜0.05)；随着饵料中小肽用量的增加，成鲤肌肉的肌苷酸含量显著提高(P＜0.05)，1.0％小肽组的IMP含量高达0.17mg／mg，比对照组提高70.0％；成鲤肌肉中的油酸和二十？

The results show that both conformations of prolyl peptide bond are not easy to transit from each other supporting the opinion that both of the BPP's bio-characters are from its conformations separately. And the function of BPP may not lie on a conformation. The other peptide bond conformations also have influence.

结果表明，脯氨酸肽键构象不易发生互变，无论哪种构象均不存在向另一种构象转变的趋势，从侧面支持了BPP具有的两种稳定的生物学作用是来自于肽键的不同构象的观点，而且BPP的生物学作用可能也不是取决于某一个肽键构象，其它脯氨酸肽键构象对其活性也存在影响。

The MAP connecting pured peptide to the lysine-tree scaffolding has less crippled peptides and better reactivity.The MAPs linked to different lysine-tree scaffolding have different immunogenicity.

将支架结构上的氨基酸作为合成的结合位点，将已合成并纯化好的线形肽作为待连接的短肽，连接到支架上，从而得到残缺肽极少的分支状八肽，效果较好。

Specifically, the invention relates to vasoactive intestinal receptor binding peptides, derivatives and analogues of vasoactive intestinal peptide, and embodiments of such peptides radiolabeled with a radioisotope, as well as methods and kits for making, radiolabeling and using such peptides for radiodiagnostic and radiotherapeutic purposes.

具体地，本发明涉及到具血管活性肠受体结合特性的肽，血管活性肠肽的衍生物和类似物，和用放射性同位素标记这类肽的实施方案，以及生产、放射性标记与使用这类肽的方法和药盒进行放射性诊断和放射性治疗。

To further testify the high efficiency of these new reagents in the synthesis of sterically hindered peptides, we successfully synthesized the extensively -N-methylated peptide moiety of the antineoplastic and lipophilic depsipeptide Dolastatin 15 using reagent BEP and FEP. We also synthesized the highly hindered tetrapeptide segment of Cyclosporin A using reagent BEMT and BEP, respectively.

为进一步检验这类吡啶正离子型缩合剂在有空间位阻的多肽合成中的性能，我们以BEP和FEP为缩合剂，成功地合成了具有抗肿瘤活性的亲脂性酯肽Dolastatin 15分子中高N-烷基化的肽链部分；分别以BEMT和BEP为缩合剂合成了生物活性肽Cyclosporin A中空间位阻最大的四肽片断。

It was found that 3 h after beginning of seizure there started a dramatic decrease in dynorphin in hilus, mossy fiber of hippocampus but an increase in leu- enkephalin in subiculum, CA1 area of hippocampus and some other limbic structures.

致痫时在海马门区及苔样纤维上强啡肽〓含量明显减少，在海马苔样纤维、〓区等结构内亮啡肽含量明显增加，电针后海马中强啡肽〓含量增加，亮啡肽含量在上述各区显著减少。

The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against H. armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of Antheraea yamamai by whole-mount immunocytochemistry, and there were three clusters cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph kept a steady level. When pupation, the titer increased promptly, but decreased to a low level after early pupal stage.

这证明了天蚕可能存在FXPRLamide家族神经肽中的DH-PBAN基因；天蚕的咽下神经节中FXPRLamide神经肽含量最高，咽下神经节有三簇细胞合成FXPRLamide神经肽，在胸神经节和腹神经节也有明显的阳性细胞；天蚕5龄幼虫的血淋巴中FXPRLamide神经肽含量比较稳定，化蛹前含量升高，蛹期含量降低。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。