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Keywords: NMT; Randomly phage display library; Affinity binding; Screening of inhibitor peptides; Molecule-screening model

中文关键词:豆蔻酰转移酶;噬菌体展示随机库;亲和结合;抑制筛选;分子筛选模型

In addition to the two axial ligations provided by His18 and Met80, the hydrogen bond and hydrophobic interactions between the heme and the protein polypeptide, the heme also covalently linked to the protein matrix through two thioether bonds which are formed between vinyl groups of the heme and cysteine residues of a classic Cys-Xaa-Xaa-Cys-His heme-binding motif of the polypeptide chain.

Cyt c是血红素蛋白中电子传递蛋白的典型代表,其辅基heme与蛋白链的结合除了来自heme轴向配体组氨酸(His18)和甲硫氨酸(Met80)的配位作用、氢键和疏水相互作用等非共价键作用方式外,血红辅基heme的两个乙烯基还与蛋白链上的结构片段域Cys-Xaa-Xaa-Cys-His中的两个半胱氨酸形成硫醚键以共价键方式相结合。

The positive peptide cleavage promoted by complex, CuⅡL and the negative response of CuⅡ/dien complex suggest the merely hydroxy pendant in complex have no ability to attack the peptide bond. The synergic carbonyl activation by metal coordination is essential for the cleavage of peptide bond promoted by CuⅡL.

比较相应铜配合物及二乙烯三胺铜配合物的切割性能,提示羟乙基侧臂单独难以实现相邻键的切割,高配位数金属中心对键羰基的活化与羟基的协同进攻是该类配合物切割多的可能机制。

Characterization of senescence-associated endopeptidase iso -zymes in wheat leaves The characterization of senescence-associated endopeptidase isozymes in wheat leaves during dark-induced senescence was performed.

而采用将明胶为底物的SDS-PAGE方法只能检测到一种新内酶同工酶的出现,因此衰老期间表达的内酶同工酶大都是对SDS敏感的或多亚基的蛋白水解酶。

Secondly, the experimental research advances of cyclopeptide nanotubes using as the templets to produce biosensors by synthesis with functional nanomaterials such as electronic, optical and magnetic ones.

首先介绍了带合适疏水性侧链的环纳米管在模拟生物跨膜离子通道方面的实验和理论研究进展,重点论及环纳米管的结构、极性和侧链的疏水性等对离子通道传输行为的影响以及分子动力学模拟研究水通道的进展。

Such mechanism may also account for the ultrastructural changes and loss of function in peptide containing afferent nerve ending. This has been suggested as an explanation for one form of desensitization, especially when peptide release was used as a measure of capsaicin-induced nerve activation.

细胞超微结构改变导致含有神经的感觉神经功能丧失也是作用机制之一;尤其是神经释放被用于测量辣椒碱介导的神经兴奋性时,该机制更被认为是神经失敏的另一种形式。

RESULTS The recombinant human prolidase expressed in COS-7 cells catalyzed the hydrolysis of organophosphorous compound soman as well as the hydrolysis of dipeptide Gly-Pro.

结果 COS-7细胞表达的人氨酰基脯氨酸二酶催化有机磷化合物梭曼的水解,也水解二化合物Gly-Pro。

The Antaisu compound therapy group, 45 patients including 20 severe patients and 25 mild patients were treated with Antaisu compound 15 tablets per day, also for 12 weeks.

复方氨素组45例,其中重型20例,轻型25例,给予复方氨素15片d^(-1)口服,共12wk。

Because relaxin leels hae been increased in women using oral contracepties, it is also suggested that relaxin may proide the missing key for understanding the association between oral contraceptie use and LBP.

由于使用口服避孕药的妇女其松弛水平也会增高,故松弛可能是理解口服避孕药使用与LBP之间联系的关键点。

In model of aromatic amino acids and the simple peptides with aromatic amino acid, intramolecular electron transfer was studied almost with pulse radiolysis technique.

含芳香氨基酸残基的简单和蛋白质模型中,研究分子内链电子转移一般采用脉冲辐解的研究方法。

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