肽

Full enzymatic synthesis of N-protected Leu-enkephalin was successfully achieved via 3+2synthetic route by α-chymotrypsin and thermolysin, and the Z-protected Leu-enkephalin phenylhydrazine was obtained in tert-amyl alcohol by thermolysin for the first time.

以3+2的合成路线使用α-胰凝乳蛋白酶和嗜热杆菌蛋白酶完成了N-保护的亮-脑啡肽的酶法全合成，并在第三戊醇中首次以嗜热杆菌蛋白酶为催化剂得到了Z-保护的亮-脑啡肽酰苯肼衍生物。

The frequency of particular amino acids in signal peptides by statistical approach was 48.54% for hydrophobic, 18.67% for uncharged, 24.54% for negative and 8.00% for positive amino acids. The most frequent amino acid was alanine, and the least was isoleucine. Three classes of signal peptides were found in the genome of P. syringae pv. tomato, 501 ORFs belong to secretary signal peptides, 36 ORFs belong to twin-arginine signal peptides, and 15 ORFs belong to lipoprotein signal peptides. Type IV pilin signal peptide and bacteriocins and pheronones signal peptide was not found in the genome.

同时，对组成信号肽的氨基酸种类作了系统的分析，发现组成信号肽的氨基酸中非极性氨基酸占48.54%，极性氨基酸占18.67%，带负电荷氨基酸占24.54%，带正电荷氨基酸仅占8.00%，出现最多的3种氨基酸依次为亮氨酸、丙氨酸和丝氨酸，最少的氨基酸是异亮氨酸，在切割位点－1端的氨基酸中83.21%均为丙氨酸，在切割位点后3位的氨基酸中最多的氨基酸也是丙氨酸。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

Methods The α-factor prepro peptide from Saccharomyces cerevisiae was used as a signal peptide to secret HBsAg in Hansenula polymorpha. Both MFα and HBsAg genes were designed based on Hansenula polymorpha-preferred condons and synthesized by overlap PCR respectively, then fused together by PCR using a pair of primers.

在HBsAg基因前加酿酒酵母α前导肽基因序列作为信号肽，将整个序列优化为汉逊酵母优选密码子，采用搭桥PCR方法分别合成后，通过1对引物PCR法融合在一起。

Neural networks based on local sequence and amino acid component with the best structures and linear equation are constructed. The results of testing to the networks indicate that the forming of cis-proline has distinct relationships with local sequence and amino acid component. A new method for peptidyl prolyl cis/trans conformation prediction based on the theory of support vector machines was introduced. The SVM represents a new approach to supervised pattern classification and has been successfully applied to a wide range of pattern recognition problems.

通过运用神经网络与线性拟合的方法，揭示了脯氨酸局部序列、肽链氨基酸组分对于顺式脯氨酸形成与否的影响，构造了基于局部序列和基于氨基酸组分的最优结构的神经网络，以及线性拟合方程，同时对顺式脯氨酸进行了筛选，结果表明，利用神经网络的方法可以提高顺式脯氨酸筛选时的信噪比，脯氨酸采取顺式还是反式构象与其局部的序列以及整条肽链的氨基酸组分均有较强的关联。

It is focal point to search possible proteinaceous differences corresponding protein peaks.

对照组/西药组的对照组2917、2784、2967、3053、3158、3842、8458、8658的峰值所代表的蛋白质主要是一些与胃肠道有关的蛋白质酶类、内源性的抗菌肽和神经内分泌系统的肽类。

Both des-Arg8, Leu8-bradykinin and HOE-140,the prototypic bradykinin B1 and B2 receptor antagonists, respectively,have been shown to reduce pain behaviors and inflammation inanimal models of persistent nociception.

des-Arg8， Leu8-缓激肽和 HOE-140，缓激肽 B1 和 B2受体拮抗剂，分别显示在动物模型中，对持续性的伤害刺激具有减轻疼痛和炎性反应的作用。

This invention relates to radiotherapeutic reagents and peptides, radiodiagnostic reagents and peptides, and methods for producing labeled radiodiagnostic and radiotherapeutic agents.

本发明涉及放射性治疗试剂和肽，放射性诊断试剂和肽，和生产这些标记的放射性诊断试剂和放射性治疗试剂的方法。

Rechallenge was performed 60 days after first tumor inoculation, all mice showed complete protection, indicating that the DC-gp100 induced specific anti-melanoma memory CD8+CTL.

为了验证NV抗原表位可以降低gp100特异的CD8+T 细胞活化阈值，我们先将NV抗原肽负载DC，接着再分别负载不同浓度梯度的gp100抗原肽，以此免疫小鼠，7天后以淋巴细胞增殖实验判断gp100特异的CD8+T 细胞活化阈值。

Object In this study we framed the refusion gene antibacterial peptide[ Cecropin A(l ~ 8)- Magainin (1 - 12),CA(1 ~ 8)-MA(l ~12),CAM] vetor and expressed it in the Escherichia.coli. Thenpurified the recombinded peptide in the Intein Mediated Purificationwith an Affinity Chitin-binding Tag-IMPACT system.

目的 构建重组基因抗菌肽[Cecropin A(1～8)-Magainin(1～12)，CA(1～8)-MA(1～12)，CAM]质粒载体并在大肠杆菌(Escherichia.coli)中表达，应用几丁质自切割分离纯化系统(InteinMediated Purification with an Affinity Chitin-binding Tag，IMPACT)分离纯化抗菌肽，并测定其抗菌活性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。