肽

Sequestrating and neutraling endotoxin activity by small molecules, especially peptides, is a prosperous field.

噬菌体展示肽库提供了一种根据亲和力高低从大量随机肽序列中筛选目标短肽的方法。

Experiment 1, let rats acquire taste preference to quinine solution, and take account of β-EP-immunoreactive neurons in different nucleuses of hypothalamic areas, separately adopted taste preference learning training and immunohistochemistry; Experiment 2, Added banana essence into quinine solution to discuss the connection between flavor preference learning and the expression of β-EP; Experiment 3, To research endogenous opiate like substances contribute to the establishment of CTP and influence the behavior of taste preference by naloxone was i.p.

实验一，采用味觉嗜好学习训练和免疫组织化学方法，让大鼠获得对奎宁的味觉嗜好，计数了下丘脑不同核团内β-内啡肽免疫反应细胞数；实验二，向奎宁溶液中加入香蕉气味，探讨风味嗜好学习与下丘脑β-内啡肽表达的关系；实验三，腹腔内注射纳络酮，探讨内阿片肽对CTP建立及味觉嗜好行为表达的影响。

To approach its" mechanism of action, we carded out this experiment. The SD rats were objects in this experiment. We made rat focal cerebral ischemia model with middle cerebral artery occlusion using a thread block technique. We gave a mark of the neurologic impairment in rat and detected the contents of calcitonin gene-related peptide and neuropeptide Y in rats" cornu ammonis and brain mantle respectively by electro-acupuncture on shuigou(DU 26) acupoint. In this way, we are trying to explore the mechanism of acupuncture therapy in cerebral ischemia.

为了进一步探讨其作用机理，本实验以SD大鼠为研究对象，采用线栓法制作大鼠局灶性脑缺血模型，运用电针水沟进行治疗，并对大鼠神经功能缺损进行评分，检测了大鼠海马及大脑皮质降钙素基因相关肽以及神经肽Y含量，从中枢神经肽类递质的变化探讨电针抗脑缺血损伤的作用机理，为临床治疗缺血性脑血管疾病提供实验依据。

They could be used not only to thesyntheses of amides and esters, but also to the syntheses of oligopeptides andbiologically active peptides, such as Leu-enkephalin, both in solution and the solid-phase.

这类缩合剂不仅可以用于酰胺和酯的合成，而且可以用于固、液相法合成小肽及生物活性肽，例如Leu-脑啡肽的合成。

In chapter 6,thorough searches on the potential energy surfaces of 5 representative tripeptides are performed and structure characteristics of the stable conformers located were analyzed and compared with that of dipeptides.

第6章中使用系统的构型寻找方法全面的研究了5种有代表性的三肽分子的势能面，并详细分析了三肽构型的结构特点及其与二肽的差异。

Finally, wetested its antibacterial activities by E.coli and S.aureus.Methods In this research, the recombinded gene was cloned into the secretion vector pTYB12 and expressed in the E.coli BL21DE3. We did the preliminary research on secretion-expression of the gene. During the purification procedure, we used the chintin beads to wash off the sundry protein, then dialysed the collecting liquid to get rid of the salinity and the other small molecular peptides.

将上述重组基因抗菌肽的氨基酸片段克隆并拼接到分泌表达载体pTYB12上转染大肠杆菌(E.coli BL21DE3)后进行分泌表达，破碎离心收集的诱导表达大肠杆菌，首次应用几丁质珠柱分离纯化系统进行分离纯化，分离洗脱杂蛋白后透析去除盐类物质及其它小分子肽，得到目的重组基因抗菌肽。

These non-phosphorylated peptides can be eluted along with phosphorylated peptides and cause the reduction of the selectivity.

这些肽段与磷酸化肽段一同被富集，降低了磷酸肽富集的选择性。

ESI-MS and HPLC were used to determine molecular weight and composition of amino acids of methionine enkephalin and to check its purity to establish its quality standard.

采用液相色谱－质谱联用仪检测蛋氨酸脑啡肽分子量和鉴别蛋氨酸脑啡肽的各氨基酸组成，反相高效液相色谱测定蛋氨酸脑啡肽含量。

Methods: Based on B-cell epitope 82~93, a multiple antigenic peptide system was synthesized as immunogen and C-57BL/6 mice were divided into 3 groups randomly. MAP group (n=5) referred to that C-57BL/6 mice were immunised with the immunogen and the polyclonal anti-serum was produced. The polyclonal antibody was identified by using an ELISA assay.

将C-57BL/6小鼠随机分为对照组、MAP组、人参总皂苷组，MAP组在预测MAGE-12的B细胞表位序列为QSDEGSSNEEQE(82-93)的基础上，采用4分枝多重抗原肽结构设计合成抗原肽，免疫C-57BL/6小鼠，产生多克隆抗体，应用ELISA实验检测多克隆抗体是否为人的抗原肽的特异性抗体。

By using lysozyme, RNase A and protein disulfide isomerase as model proteins, we present a hypothesis that protein cross-linking can be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links.

我们以溶菌酶、核糖核酸酶A和蛋白质二硫键异构酶为模型对异肽键进行了体外详细的研究，提出了解释异肽键形成机理的假说：1蛋白质发生构象变化包括二级结构的改变；2蛋白质形成分子间二硫键：3二硫键的形成帮助蛋白质形成了分子间异肽键。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。