肽

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

Hemolysis increases insulin degradation but does not affect C-peptide level, and because C-peptide and insulin are secreted in equimolar amounts, the researchers used cord-blood serum C-peptide level rather than insulin level for the index of fetal β-cell function.

溶血增加了胰岛素的降解，但却不影响C-肽水平，同时C-肽和胰岛素又是等量分泌的，因此他们选择了测定C-肽而非胰岛素来衡量胎儿β细胞功能。

One of the synthetic methods of the MAP is to connect subsequent amino acid one by one with the lysine belonging to the lysine-tree scaffolding. Because of the steric hinderance effect, synthesis becomes more and more difficult. And for so many crippled peptides in the MAP, the reactivity of it is not so good.

将支架结构上的氨基酸作为合成的结合位点，按照常规方法依次进行后续氨基酸的连接，由于空间位阻效应，后续氨基酸的连接困难度增加，八分支肽中的残缺肽较多，由此方法合成的分支肽反应活性较低。

The method uses Acaudina Molpadioidea collagen peptide as ACE inhibitory peptide, through repeated testing, the inhibitory peptide activity in vitro by HPLC detection with good analysis effects through direct injection and isocratic elution is obtained.

本方法以茄参胶原肽作为ACE抑制肽，通过多次试验，得出一种直接进样、等度洗脱便能达到良好分析效果的HPLC测定该抑制肽体外活性的方法。

In this analogue, the hydrogen of amide group could still form a hydrogen bond with the oxygen of ester bond and the ester bond was isosteric with peptide bond.

我们合成了甜味肽的类似物L-天冬酰氨酰-L-α-羟基苯丙酸甲酯，将甜味肽的天冬氨酸替换为天冬酰胺，肽键替换为酯键。

The former is necessary for equilibrium-controlled synthesis of peptide bonds while the latter is necessary for kinetically controlled synthesis of peptide bonds.

酰胺酶活性起水解或合成肽键的作用，因而对平衡控制的肽键合成是必需的；酯酶活性起转移酰基的作用，因而对动力学控制的肽键合成是必需的。

Results Micrographic observation revealed that the Jiean-peptide made the hyphal expanded and globular.

结果 显微形态观察表明，经捷安肽素处理后，供试真菌呈现球形膨胀，而且随着捷安肽素作用强度增强，球形膨胀的程度和发生频率也增加，提示捷安肽素的作用位点在真菌细胞壁。

The lipid moity is the molecular"endotoxic principle", and highly conserved among all genus. Lipid A is the ideal target for developing endotoxin neutralizer.

用小分子中和内毒素活性是一个活跃的研究方向，高亲和力是小分子中和内毒素活性的前提和关键，小分子分为肽类和非肽类，肽类是研究的主要方向。

Furthermore, influences of different phosphate salts at various concentrations on the ionization efficiency of phosphopeptide were investigated systematically, finding that the signal intensity for phosphopeptide 48FQEEQQQTEDELQDK63 digested from β-casein were 5 to 8 times increased in an optimized condition with 10 mmol/L ammonium monobasic phosphate or 3 to 4 times increased with 10 mmol/L ammonium dibasic phosphate as additive to matrix 2,5-dihydroxybenzoic acid, respectively.

考察了两种适合于磷酸化肽离子化的基质类型2，5-二羟基苯甲酸和2，4，6-三羟基苯乙酮。用2，5-二羟基苯甲酸作为基质时，当加入10 mM 磷酸氢二铵时，磷酸化蛋白质β-casein的磷酸肽 48FQEEQQQTEDELQDK63的离子化效率可以增强5-8倍，当加入10 mM磷酸二氢胺时，磷酸肽的离子化效率可以增强3-4倍。

To elucidate the structural features and the antimicrobial activity of this fragment, we re-designed and synthesized two peptides corresponding to the main mimic structures of Mytilin-1 from Mytilus coruscus, we named these two peptides Mytilin Derived Peptide-1 and Mytilin Derived Peptide-2, respectively.

为验证该区域是否具有抗菌活性，通过对厚壳贻贝Mytilus coruscus抗菌肽Mytilin进行空间结构模拟，选取其中β-发夹部分肽段，采用了固相化学合成的方法合成了两条10肽，分别命名为Mytilin Derived Peptide-1 (MDP-1)和 Mytilin Derived Peptide-2 (MDP-2)。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。