肽

The compounds 1～6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin andphalloidin.As the content of compound7, compound 8 and compound 9 were all to little.

化合物1～6分别为：β-鹅膏毒肽、α-鹅膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

In all, A.subjunquillea.S.lmai methyl alcohol crude extract were research by high pressure liquidchromatography,.separation and purification obtained to 9 kind of compounds, identified 6 kind ofcompounds. The compounds 1～6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin and phalloidin.

总之，黄盖鹅膏甲醇粗提物应用高效液相分离纯化9种单体化合物，鉴定6种分别为β-鹅膏毒肽、α-鹅膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

Methods Mimicry and speculation on possible displaced amino acids in proper positions of peptide increased the biological effects of TNFR blocking peptide by compote modeling. The original peptide and its mutant were synthesized. The competitively inhibitory effects on green fluorescence protein-tumor necrosis factor fusion protein with TNFR were compared between original peptide and its mutant (receptor competitively inhibitory binding assays). The inhibitory effects on cytotoxicity were compared between original peptide and its mutant.

通过计算机模建，模拟并推测提高TNFR封闭肽生物学效应需置换的位点和氨基酸；合成原封闭肽和突变肽；受体竞争抑制结合实验比较原肽与突变肽对绿色荧光蛋白－肿瘤坏死因子融合蛋白结合TNFR的竞争抑制效应；细胞毒作用抑制实验比较原肽与突变肽对TNFR的封闭效应。

It was demonstrated that substance p,calcitonin gene related peptide,neurokinin A and neurokinin B immunoreactivities in circulating blood,nodose ganglion,C7-T5 spinal ganlia and dorsal horn and solitatary nucleus were increased in the asthmatic guinea pig than in normal control.

测得哮喘豚鼠结状神经节，C7～T5段脊神经节，C7～T5段脊髓后角和孤束核内P物质，降钙素基因相关肽，神经激肽A和神经激肽B水平明显高于对照组P.05。测得哮喘患者血浆P物质，降钙素基因相关肽，神经激肽A和神经激肽B含量高于正常对照组P.05。

For a peptide to act as part of the host defense system or to be of pharmaceutical interest, high antimicrobial activity must be combined by low hemolytic activity. Our results indicated that the antimicrobial activity increased with increasing charge and polar angle, with charge of ＋4 and polar angle of 140° exhibiting the highest antibacterial and membrane permeabilizing activity. Higher hydrophobicity and hydrophobic moment correlated with increased hemolytic activity, thus decreasing selectivity dramatically.

胜肽要扮演宿主免疫机制的一部分或要成为新型态的抗生素不仅要有强大的抗菌活性还需要有高度的选择性，由本实验的结果发现，当抗菌胜肽的正电荷及极性角度增加时，胜肽之抗菌活性会随之增加，当抗菌胜肽的电荷为＋4而极性角度为 140°时，胜肽有最强之抗菌能力及膜渗透活性；而随著胜肽疏水性以及疏水性矩的增加，会增加溶血活性，结果反而降低了抗菌胜肽的选择性。

In this article, the advance in research on tumstatin active peptides and their derivatives are introduced and the bioactivities of Tum-5, T7 and 19 peptides and the derivants of these active peptides linking with NGR sapience or human IgG3 hinge region are summarized in detail.

本文详细介绍了肿瘤抑素活性肽段及其衍生物的研究进展。对Tum-5、T7肽和19肽以及这些活性肽段连按NGR导向肽或人源性IgG3铰链区等肽段后形成的衍生物作了较详细的介绍。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.

采用氯乙酰基保护天冬氨酸，然后进行氨解生成GD二肽，利用猪胰脂肪酶催化合成RGD三肽，着重解决了亲水氨基酸/肽片段底物的溶解性与合成产率低的问题，建立了一种新的有效合成RGD三肽的反应模型，并探索解决了极性较强的氨基酸的酶促肽合成的问题，对脂肪酶催化合成肽的影响因素从理论上作了较全面的分析，为肽片段的缩合反应提供了一种新思路。

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

It suggested that the AEBSF delayed leaf senescence though inhibition the endopeptidase activity and influence the SOD and POD activity.Native PAGE with gelatin as substance in gel showed the activities of isoenzyme 3 and 4 were inhibited partly by AEBSF treatment on leaves.It implies that the two isoenzymes are type of serine endopeptidase and may play an important role in cucumber leaves senescence. Degradation of protein and changes of endopeptidase isoenzymes in cucumber Cucumis sativus L.

活性电泳显示，黄瓜叶片中检测到6条内肽酶同工酶，其中4条(CEP2，3，4，6)为丝氨酸型内肽酶，内肽酶抑制剂AEBSF抑制了叶片中丝氨酸内肽酶同工酶的活性，而ABA使丝氨酸内肽酶CEP2，3，4的活性显著增强，再次暗示了丝氨酸型内肽酶在黄瓜叶片衰老以及叶片Rubisco降解中具有重要作用，而且除了丝氨酸内肽酶外，可能还有其它因素参与了衰老的调控。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。