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On the other hand, after 7-week gavaging abidingly, the effect of II-RMeOH on the bleomycin-induced chronic pulmonary fibrosis was investigated. The study was evaluated by measuring the hydroxylproline, collagen, transforming growth factor-β1 (TGF-β1), and microwave Masson's trichrome stain.

经由肺脏检体的组织切片染色后,可以用半定量型态学分析来判断肺脏的发炎情形;经过判读后,结果和测定出来的生化值有相同的趋势,在投予板蓝根甲醇粗抽物后,减轻了肺脏的发炎程度。

In situ hybridization showed that CYP11B2 mRNA was localized in the endoplasm of liver fat -storing cell and typeⅡ alveolar cells of the lung .

结果 用RT-PCR及Southern Blot法证明了肝脏和肺脏能表达CYP11B2,原位杂交表明CYP11B2 mRNA定位在肝脏的伊藤细胞及肺脏的Ⅱ型肺泡上皮细胞的胞浆内。

Lung concentration-time data after oral administration of 50mg/kg body weight we re also best d escribed by a two-compartment open model. The parameter of male chicken were as following: t1/2 a 为 2.28S±0.657h , t1/2Ka1.090±0.193h, t1/2B 为 37.606±4.474h, AUC530.797±48.050*h, CL 0.095±0.008mg/kg/h/, Tmax 5.256±0.442h, Cmax 9.044±0.016ug/g.

公鸡肺脏主要的药代动力学参数:t1/2 α为2.285±0.657h、t_(1/2Ka)为1.090±0.193h、t_(1/2β)为37.606±4.474h;AUC为530.797±48.050~*h、CL为0.095±0.008mg/kg/h/、T_为5.256±0.442h、C_为9.044±0.016μg/g;母鸡肺脏主要的药代动力学参数:t_(1/2α)为1.556±0.825h、t_(1/2Ka)为0.732±0.101h、t_(1/2β)为23.304±2.320h;AUC为395.073±12.095~*h、CL为0.127±0.004mg/kg/h/、T_为3.584±0.440h、C_为10.691±0.542μg/g。

The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.

免疫电镜观察结果显示,大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒,在上皮细胞间还可见有DNES细胞;肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应,肺脏内也可见DNES细胞。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系,应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后,流式细胞仪技术检测CCR1和CCR2的表达,ELISA法检测细胞培养上清液内白介素-8的浓度;体内实验选用C57BL/6J小鼠,腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg),LPS刺激4 h后收集BALF和肺脏标本,计数BALF内细胞总数,应用BCA法检测BALF内蛋白浓度,Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

ConcLusion:Our studies show that Helical Tomotherapy plans can get better dose uniformity,dose gradient and savage for cord,heart,esophagus and trachea than intensity modulated radiation therapy plans,but the V5 and V10 of the ipsolateral lung,the heterolateral lung and the total lung are higher than IMRT plans.

本组病例结果表明,螺旋断层放疗计划较常规直线加速器静态调强放疗计划有更好的剂量均匀性及更陡峭的剂量梯度,可更好地保护心脏、脊髓、食管和气管,但患侧肺脏、健侧肺脏和全肺的V5和V10均高于IMRT计划。

Hours after infusion, the radioactivity accumulation appeared in lung, and it revealed that the infused cells distributed mainly in lung, and some appeared in liver, spleen, kidney and bladder. 6 hours after infusion, the radioactivity in lung diminished compared with that at 3 hours after infusion, and bilateral kidneys imagine appeared intensively. 9 hours after infusion, the radioactivity in lung appeared reduced significantly than that at 3 hours after infusion, and liver imagined increasedly, intestine tract imagine appeared in several rats.

本研究中,静脉移植MSCs后3h,肺脏放射性浓集,移植细胞主要分布在双侧肺部,肝脏、脾脏、肾脏和膀胱分布较少;移植6h后,肺脏放射性较移植3h时减弱,双侧肾脏显像明显增强;移植9h后,肺脏放射性较移植3h时明显减弱,肝脏显像增强,个别大鼠肠道出现显像;移植18h后,此时肺脏显像明显稀疏,肝脏和脾脏显像显著加强。

In this study, we evaluated the effect of Isatis indigotica radix extract in experimental murine model against pulmonary fibrosis.

经由肺脏检体的组织切片染色后,可以用半定量型态学分析来判断肺脏的发炎情形;经过判读后,结果和测定出来的生化值有相同的趋势,在投予板蓝根甲醇粗抽物后,减轻了肺脏的发炎程度。

The group B presented lots of petechiae since the fifth week; the surface of right lung began less petechiae; during 26th week, the symptom of reduced volume and hard surface was less than before.

大体标本观察发现,单纯照射组大鼠于照射第5周时见肺脏肿胀、表面鲜红,并有暗红色斑片状出血;第12周时肺肿胀减退,表面瘀斑颜色转暗;第26周时其肺脏体积缩小、质地变硬,可见苍白实变灶。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂,有的出现肺大泡,肺泡内伴有渗出及少量非出血,肺组织可见咽细胞浸润;血清中AMY、ALP、TNF-α、IL-6明显增高;在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加;NF-κB在SAP大鼠肺脏中表达增加,并且出现核浓染,说明NF-κB前移入核;启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

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