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Methods The Cpn-Ag was detected by direct immunofluorescence using self-made monoclonal antibody of Cpn for 50 archival paraffin-embedded coronary artery atheroma specimens and 20 normal coronary artery specimens.

采用自制肺炎衣原体单克隆抗体的直接微量免疫荧光法检测50例冠状动脉瘤标本和20例正常冠状动脉标本中Cpn-Ag的表达。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

We've worked to prevent the spread of potential pandemic diseases like avian flu and SARS.

我们致力于阻止潜在的像禽流感和非典型肺炎这样的疾病在全国流行。

But in the developing world, about sixty percent are caused by bacteria .

而在发展中国家,60%的肺炎是由细菌引起的。

Mao Xi is bronchitic as a result occurence rate of disease of blood of little patient low natrium is apparent prep above is common and pneumonic group (P.01); Mao Xi is bronchitic standard of natrium of serum of little patient of serious illness group is clear under light disease group (filling natrium of bronchitic interpose group treats Mao Xi of 20 Mmol/L of P.01); serum natrium 48 H hind and did not intervene group.

结果毛细支气管炎患儿低钠血症发生率明显高于普通肺炎组(P.01);毛细支气管炎重症组患儿血清钠水平明显低于轻症组(P.01);血清钠20 mmol/L毛细支气管炎干预组补钠治疗48 h后和未干预组病情评分差异有统计学意义(P.01);氢化考的松治疗组低钠血症发生率低于未应用氢化考的松组(P.01)。

The bronchopneumonia is exudative in type and lobular in distribution.

支气管肺炎在性质上是渗出性的,在分布是小叶性的。

Aspiration of gastric contents may produce a severe chemical pneumonitis with considerable pulmonary oedema and bronchospasm (Mendelson's syndrome).

吸入胃内容可以产生严重的化学性肺炎,有相当程度的肺水肿及支气管痉挛(Mendelson's综合征)。

A severe pneumonia and arthritis emerged in calve herds introduced from interstate to Chongqing and other three provinces.

重庆等4省市从外省引进的肉牛群发生以严重肺炎和关节炎为主要表现的疾病。

Results We know from the chest X-rays that the infant thymus is organ with the most change and it's usually looked like lobar-pneumonia,cardiomegaly and mediastinal tumor so that it brings much trouble to pediatrician and radiologist.

结果 婴幼儿胸腺是胸片中变化最多的器官,常酷似大叶性肺炎、心影增大、纵隔肿瘤等,给影像科医生和儿科医生带来不少的困扰。

Mr. Pneumonia was not what you would call a chivalric old gentleman.

肺炎先生不是一个你们心目中行侠仗义的老的绅士。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。