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The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明,制得的兔抗PRRSV IgG纯度较高,具有良好的特异性,可用于原位检测病猪肺组织中PRRSV抗原的分布;Envision法的检测结果显示,PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内,其次为肺泡上皮细胞和细支气管黏膜上皮细胞;通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测,均有较高的阳性细胞表达,平均阳性细胞率为46.5%。

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The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因,如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务,你就做你的事好了。

Who is in possession of this?

这是谁的?