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With optimized ELISA method, sera from 443 cases, including 68 patients with acute and 189 with chronic schistosomiasis, and 100 normal human controls, were tested, and the sensitivity, specificity and cross-reaction were also evaluated, respectively.

共检测血清443份(急性血吸虫病患者血清68例,慢性血吸虫病患者180例,正常人100例,肺吸虫感染20例,华支睾吸虫感染48例和钩虫感染者血清27例),进行敏感性,特异性和交叉反应性分析。

The double antibody sandwich ELISA system was established for diagnosis of acute and chronic schistosomiasis. We found that the positive detection rates of this method for acute and chronic schistosomiasis achieved 79.4% and 64.4%, and the specificity was 92%. The rates of cross-reaction with paragonimiasis, clonorchiasis, and hockworm infections were 20%, 20.8%, and 14.8%, respectively.

成功建立体系IgG-IgY ELISA,用于检测急性血吸虫病患者血清和慢性血吸虫病患者血清的阳性率分别为79.4%和64.4%,最低灵敏度为9.3ng/ml,检测正常人血清的特异性为92%,与肺吸虫、华支睾吸虫、钩虫患者血清的交叉反应性分别为20%,20.8%,14.8%。

If a respiratory infection is caused by lung flukes orcanine nasal mites, a course of anti-parasitic medications areprescribed.

如果呼吸道感染引起的肺吸虫或犬鼻螨,课程杀伤寄生虫药物的处方。

Results The CT findings of pulmonary distomiasis included:(1)unilateral or bialteral mottling,patchy and nodular shadows with hazy border.

结果 肺吸虫病肺内CT表现包括:(1)单侧或双侧肺野出现斑片状、结节状影,边缘模糊,部位、状态易变,少数病例可见特征表现的&隧道&征;(2)肺门影增大,肺纹理紊乱或病灶呈成簇状窟穴状影;(3)胸腔积液常见,特征表现为交替性胸腔积液和心包积液。

Objective:To study the CT features of paragonimiasis of the lung.

目的 :探讨肺吸虫病的胸部CT表现。

Objective To study CT features of cerebral paragonimiasis and the values of CT examination.

目的 探讨脑肺吸虫病的CT表现及CT检查价值。

Objective To discuss the CT and MRI findings of acute cerebral paragonimiasis and evaluate the recognition of the disease.

目的 探讨肺吸虫脑病急性期的CT及MRI表现,提高对该病的认识。

Methods:The CT features of 32 cases of paragonimiasis of the lung confirmed clinically were analysed.

对 3 2例经临床证实的卫氏肺吸虫病的临床与胸部CT所见进行回顾分析。

We have obtained the anti-SEA IgY antibody, established and optimized the double antibody sandwich ELISA system to detect SEA of S. japonicum from the sera of acute and chronic patients with schistosomiasis and normal persons. The cross-reaction with sera of paragonimiasis, clonorchiasis and hookworm infections were also investigated. Our results demonstrated that the IgY-ELISA method was effective in evaluating the therapeutic effect in different parasitic burden of infected mice, which provides a novel technique for clinical diagnosis of S. japonicum infection.

本研究成功制备并鉴定了抗SEA的IgY,进行了理化性质的研究,成功建立且优化了IgG-IgY ELISA检测体系,用于正常人,急性血吸虫病患者血清和慢性血吸虫病患者血清的检测,以及肺吸虫、华支睾吸虫、钩虫患者血清的交叉反应性的分析,对感染血吸虫的小鼠进行了疗效考核,为血吸虫病的诊断提供了有效的辅助诊断方法。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增,扩增产物经琼脂糖凝胶电泳后分析结果。

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