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The carboxyhemoglobin levels of in-flowing pulmonary blood and out-going pulmonary blood were determined at 2,4 and 6 hours after treatments. Malondialdehyde contents and superoxide dismutase activities in the lung,liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope,and immunohistochemical technique was used for analysis of heme oxygenase-1 (HO-1) protein expression and distribution in lung and liver.

各组分别在制模后2、4和6 h测定出入肺血中碳氧血红蛋白水平;肺、肝组织及血液中丙二醛含量及超氧化物歧化酶活性;光镜下观察肺、肝组织形态学改变;免疫组化分析血红素加氧酶-1(HO-1)在肺、肝组织中的蛋白表达和分布。

The anastomosing occurred between the pleural superficial and the subpleural vascular networks on the capillary level or the precapillary level. The honeycomb vascular networks were also various in size and form at a low magnification. The venule connected with the pulmonary capillary networks directly at a high magnification. The single capillary networks were found in the alveolar septa. The longitudinal crests and dense round imprints of endothelial nuclei and the clear circular constrictions were found on the surface of the larger vessel in the lung parenchyma. The round imprints of endothelial nuclei also occurred on the subpleural venule and capillary.

胸膜面浅层的微血管与胸膜下微血管之间形成毛细血管水平或毛细血管前水平的吻合;低倍镜下肺实质内微血管网也呈现大小不等、形态各异的蜂窝状结构;高倍镜下肺泡毛细血管与微静脉之间联系紧密;在肺泡隔内毛细血管网成单层分布;肺实质内管径较大的微血管表面可以见到纵行的嵴状结构和密集的内皮细胞核的压痕,以及明显的环形缩窄;胸膜下微静脉和毛细血管表面可见圆形内皮细胞核的压痕。

A total of 50 couples allogene rats were performed same proceeding like that.

供体经过肝素化后,在0~4℃条件下取心肺,并作适当修剪,最后在0~4℃条件下将心肺供体SD大鼠的主动脉与受体Wistar大鼠的颈总动脉行端侧吻合,并进行心肺复苏。

Results 6 patients with PAP presented with ground-glass opacification of the bilateral lungs on HRCT. Typical chest HRCT scan showed "map-like" lesions in 3 cases and "cobble stone-like" lesions in 3 cases. Multifocal consolidative opacities in the lungs were seen on HRCT in 3 cases. Lung biopsies from 6 patients with PAP revealed a prominent positive periodic acid-Schiff intra-alveolar exudates. There were normal alveolar spetasis in 3 patients while interstitial thickening, fibroblast hyperplasia, interstitial fibrosis in the others.

结果 6例患者在不同层面可见磨玻璃影,其中2例可见小结节影;3例表现为两肺斑片状磨玻璃影与周围肺组织分界清楚,呈地图样改变;3例因磨玻璃影与小叶间隔增厚交织成铺路石样改变;3例可见肺泡实变融合成密度较高的斑片状阴影,1例在肺泡实变区可见&空气支气管征&。6例患者活检肺组织在光镜下显示肺泡腔内充满大量块状或颗粒状嗜伊红物质,PAS染色阳性,AB染色阴性,其中3例肺泡间隔正常,3例肺泡间隔增宽,可见慢性炎性细胞浸润、成纤维细胞增生和胶原沉积。

The intersegmental veins can be considered as the main tributary of pulmonary segmental veins and it commonlly located in the inferior, interior and posterior side of the corresponding segmental bronchi.

肺段动脉与支气管紧密伴行,并有基本一致的分支和分布,多位于支气管的上、外或前方;肺段静脉不与支气管分支一致,可分段内支与段间支,后者可视为肺段静脉的主干,多位于肺段支气管的下、内或后方。

Objective CT-guided percutaneous transthoracic fine needle cutting biopsy was performed in small solitary pulmonary nodulus,to find out the useful way for diagnosis of the pulmonary lesion earlier.

目的 对肺孤立性结节行CT定位导引下肺穿刺切割活检术,探讨早期确诊肺孤立性结节的有效方法。

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内,让其自由泳动自然溺死,除溺水致死组外,各组动物分别在规定的时间点断头处死,行体重和血氧饱和度等指标检测,并在冰盘中迅速开胸取肺,准确称重以计算肺系数,观察各组大鼠肺脏外观,取肺组织福尔马林固定,制成病理切片HE染色,光镜下观察肺组织病理改变;用免疫组化的方法检测肺内IL-8、IL-10的表达,并对其相关性进行分析研究。

Results Thirty-one(7.64%) of 406 patients showed abnormal ventilatory function, while 165 of 404 patients showed diffusing abnormality. Of the 434 patients who had received HRCT scans,151(34.79%) showed abnormalities including subpleural and basal ground-glass and reticular attenuation,nodular septal thickening and bronchiectasis. Of the 395 patients who had received both lung function test and HRCT scanning,55(13.92%) had abnormalities both in the lung function and HRCT.

结果 在4 0 6例进行肺通气功能检测的患者中,31例(7 6 4 %)异常;4 0 4例进行肺弥散功能检测,其中16 5例(40 84 %)异常;4 34例进行胸部HRCT检查,15 1例(34 79%)异常,表现为磨玻璃密度阴影、网格状阴影和胸膜下弧线影等;395例同时接受肺弥散功能检测和胸部HRCT检查,5 5例(13 92 %)两项均异常,且HRCT显示双肺病变典型者的肺弥散功能较HRCT正常者有显著的下降,两组差异有统计学意义。

In this experimental conditions, the VEC had not damnified obviously by perfusion with IECS in 4 hours. The pulmonary vascular resistance could decreased by POE1 and so vascular endolhelial cells would be protected. Mannitol not only increase osmotic pressure but also cleanse OFR. The addition of mannitol, POE1, glucose and osmotic pressure to IEC solution contributes to reduce the quantities of OFR.

2在本实验条件下所采用的IECS进行犬肺灌洗、离体保存4h之内肺血管内皮细胞无明显损伤,PGE;具有保护肺血管内皮细胞的作用;甘露醇具有增加渗透压和清除自由基的作用;灌洗前肺动脉内注射PGE;及增加 ECS渗透压和代谢底物、提高PH值使之略微偏碱均有助于抑制供体肺离体、低温、冷缺血保存期间OFR的产生。

Results:Compared with the normal controls, manifestations of severe right heart failure,well-elevated pulmonary arterial pressure and serious right heart hypertrophywere observed in the pulmonary hypertensive rats 28 days after left lungresection and MCT injection.

结果:与对照组相比,MCT 注射后28 天大鼠出现重度右心衰竭征象,平均肺动脉压、右室肥厚度均明显升高;镜下可见明显肺血管重构,表现为右肺纤维化、肺小动脉内皮和中膜平滑肌层增生、血管密度降低;肺组织中HGF、eNOS 表达明显减少、TGF-β和ET-1 显著升高(P0.05),这些细胞因子表达量的改变与肺血管重构存在着明显的相关性。

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