肥大细胞

METHODS: Cultured rat cardiomyocytes were induced to be hypertrophy by angiotensin Ⅱ and norepinephrine. Glucose oxidation rate, glucolysis rate and fatty acid oxidation rate were determined by liquid scintillation counting, and cell apoptosis was detected by TUNEL.

应用血管紧张素Ⅱ(AngⅡ， 0.1mol/L)+去甲肾上腺素(NE 1mol/L)诱导培养大鼠心肌细胞肥大，以同位素液闪计数法测定葡萄糖有氧氧化率、葡萄糖酵解率和脂肪酸有氧氧化率，TUNEL法测定细胞凋亡。

Results: The renal perfusion was increased in diabetes group after the injection of alloxan. The PSI, AUC and ascending slope rate of the curve of the time-intensity curve were increased in 1w and 2w with decreased of HPT; the pathological examination exhibited glomeruli hypertrophy and no thickening of basal lamina. The PSI and AUC in 4w and 6w were more than that of control group; the PIT and ascending slope rate of the curve got closed to normal in 4w and 6w; the intercapillary cells increased. The speed of ascending slope rate of the curve in 8w, 10w and 12w was slower, shown as PIT and HPT delayed; the pathological examination showned increasing of mesangial cell, hyperplasia of mesangial matrix and thickening of basal lamina, followed with obliteration of partial micrangium, glomerular sclerosis and reduce of PSI in 12w.

结果：糖尿病组兔注射四氧嘧啶后肾皮质血流灌注增大，1周、2周表现为时间-强度曲线参数中峰值强度升高、曲线下面积增大、达峰时间缩短、曲线上升斜率升高，此时病理可见肾小球肥大，系膜基质正常，基底膜无增厚；4周、6周，峰值强度、曲线下面积高于对照组，达峰时间、曲线上升斜率接近正常，系膜细胞增多。8周、10周、12周，达峰时间延长、曲线上升斜率减慢、峰值强度减半时间延长，病理出现肾小球系膜细胞明显增多，系膜基质增生，基底膜增厚，至12周病理出现部分毛细血管管腔闭塞，肾小球节段性硬化，峰值强度测值减低。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

Result］All of enzyme tests were normal.The author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

Result］all of enzyme tests were normal.the author could observe the dissolved degeneration of part of sacrospinal muscle fibers,muscle fiber hypertrophy,and a small quantity of inflammatory cell infiltration with a light microscope.focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察。［结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

The author could observe the dissolved degeneration of part of sacrospinal muscle fibers, muscle fiber hypertrophy, and a small quantity of inflammatory cell infiltration with a light, microscope. Focal solution of muscle fiber, the aggregation of mitochondria around the nucleus, the increase of lipid droplet and lysosome in cyle, and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope. Pathogenesis of chronic lumboscaral compartment syndrome may be as followed.

术中切取竖脊肌标本用于组织病理学观察和透射电镜观察［结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灯状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

AngiotensionⅡand endothelin-1 are two important vasoactive peptide,having powerful vasoconstricting and hemodynamic function,except this,both have nonhemodynamic effects as growth factor to induce proliferation,hypertrophy,to stimulate synthesis of matrix proteins,furthermore,also to inhibit matrix degradation, even to mediate inflammation reaction.

血管紧张素Ⅱ(angiotensinⅡ，AngⅡ)和内皮素1(endothelin1，ET-1)是两个重要的血管活性激素，除了血流动力学作用外，还有非血流动力学作用，如促进细胞增生、肥大，刺激细胞外基质的合成并抑制其降解，介导炎症反应。

The bones were harvested, then bones length and length of their diaphyses were measured under stereo microscope. The histomorphometric analyses on midlongitudinal sections of bones were performed.

16d胎龄雌性小鼠尺骨在含有葛根素的BGJb培养基中旋转培养4 8h后，测量骨总长和骨干长度；HE染色，光镜下计数破骨细胞和肥大软骨细胞。

The ulnas of fetal mice were cultured for 48 hours in BGJb medium treated respectively with estradiol and purearin. After fixing long bones and decalcification, paraffin sections were made, and then performed immunohistochemical staining for determining the level of typeⅠcollagen protein in growth plates. Positive cell locations in the resting zone, proliferative zone and hypertrophic zone of epiphyseal plate were observed under light microscope.

剥离出胎鼠尺骨，置于BGJb培养基中，葛根素和雌二醇作用48 h后，将长骨固定，脱钙后作石蜡切片，进行免疫组织化学染色，光镜下观察骺板静止区、增殖区和肥大区阳性细胞定位，图像分析系统下测定免疫反应阳性细胞面积。

Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。