肥大细胞

Candida colonisation did not enhance oalbumin permeation in mast cell deficient W/W mice but did in congenic littermate control +/+ mice. Reconstitution of mast cells in W/W mice by transplantation of bone marrow deried mast cells restored the ability to increase oalbumin permeation in response to Candida colonisation.

念珠菌定植不加强肥大细胞缺陷W/W小鼠的蛋白通透性，而其同系同窝对照+/+小鼠则有加强，W/W小鼠通过骨髓肥大细胞移植重建肥大细胞功能可恢复念珠菌定植后对卵白蛋白通透性的增强作用。

Results: HE stain showed the obvious thicken of the artery, RV/ and mPAP elevated obviously (P.05) in model group in comparison with the control group, they were also elevated obviously (P.05) in the model group compared with the treatment group. The tryptase + mast cell and the chymase + mast cell obviously increased in the model group compared with the control group, also increased in the model group compared with the treatment group.

平均肺动脉压力模型组动物较对照组明显升高(P.05)，模型组较治疗组也明显升高(P.05)；右心室／模型组较对照组明显增加(P.05)，模型组较治疗组也明显增加(P.05)，肥大细胞总数（类胰蛋白酶阳性的肥大细胞）及类糜蛋白酶阳性的肥大细胞模型组较对照组明显增多(P.05)，肥大细胞在治疗组较模型组明显减少(P.05)。

Results: Mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. After ovum implantation ,the mast cells were comparatively less at the place of implantation. The number of mast cells of early and late pregnancy(1～5 dyas, 15～19 dyas) was apparently higher than that during middle pregnancy(P＜0. 05). Stained by alcian blue-safranin, both red and reddish blue mast cells decreased gradually in number during pregnancy, but the proportion of blue mast cells was increased. Critical electrolyte concentration of mast cells decreased as time went on.

结果：(1)肥大细胞主要分布于子宫肌层，子宫内膜较少，且胚泡植人部位的肥大细胞明显少于非植入部位；(2)妊娠早期(孕1～5d)和妊娠后期(孕15～19d)肥大细胞数显著多于妊娠中期(P＜0.05)；(3)Alcian蓝藏红组化染色后，可见妊娠过程中红色和红蓝混合色肥大细胞逐渐减少；蓝色肥大细胞所占比例逐渐增大，孕9～19d肥大细胞几乎全部为蓝色；(4)肥大细胞临界电解质浓度值随妊娠时间的推移而变小。

The results indicated that there were only single mucosal mast cell sunset in rat mammary gland.They had various shapes.Most of mast cells were located in the interalveolar stroma and intralobular connective tissue.

结果 ：AB- S染色显示，大鼠乳腺只存在黏膜型肥大细胞；无论是泌乳期还是静止期，乳腺肥大细胞大多分布于腺泡间和小叶间结缔组织中，细胞的形态各异，但细胞数量在静止期和泌乳期有显著差异，泌乳期的乳腺肥大细胞数量明显减少(P 。0 1)。

In conclusion, like human mast cells, bullfrog mast cells also contain the special enzyme, tryptase and it was likely that bullfrog can be used as an ideal experimental animal for the study of mast cell biology.

研究证实，与人类肥大细胞相似的是牛蛙肥大细胞也含有这种特有的类胰蛋白酶，牛蛙有可能是用作肥大细胞生物学研究的理想实验动物。

The histamine in the particles will increase the transmission capacity of capillary and the velocity of the tissue-fluid flow and the superficial shear stress of related mast cell will be increased, which will once again activate mast cells to discharge active substance. Part of the active substance will be absorbed by capillary and the other part will be passed by tissue fluid to a remote place and it will activate the mast cells and the capillaries there. The directional path of the tissue fluid is approximately the same as the meridian, which forms the phenomenon of feeling-along-meridian on macroscale.

脱颗中的组胺等物质会引起毛细血管壁通透性的增加，进而引起组织液流动速度的增加和肥大细胞表面的切应力加大，这样会进一步促进肥大细胞的激活，从而释放更多的活性物质，这些物质一部分被静脉端毛细血管吸收进入血液，另一部分则随着组织液的定向流动向远处，并进一步刺激远处的毛细血管和肥大细胞，而组织液的定向流动途径和传统的经络线走向基本吻合，在宏观上就构成了循经感传现象。

The topics include: development of mast cells and basophils, IgE-mediated activation of mast cells and basophils, mechanisms of exocytosis, non-IgE-mediated activation and modulation of mast cells and basophils, description of mediators including those that are lipid-derived, proteases, proteoglycans and cytokines.

文章讨论的课题包括：肥大细胞和嗜碱性粒细胞的发育，IgE介导的肥大细胞和嗜碱性粒细胞的活化，胞吐作用的机制，非IgE介导的肥大细胞和嗜碱性粒细胞的活化和调节，这些课题中涉及到的介质包括：脂类衍生物，蛋白酶、蛋白聚糖和细胞因子等。

Concludes:Three subsets of mast cell in human tissue are sorted and depurated with antibodies of specific enzyme and immunofluorescence labelling and flow cytometry. Mast cell of containing profuse secretary vacuoles could be displayed by confocal microscopy. It demonstrate that mast cell have material substructure offering for fast reaction of I type allergies of human being.

利用肥大细胞的特征性酶抗体、免疫荧光标记和流式细胞仪可将人组织中的肥大细胞分选纯化为三种亚型；以共聚焦显微镜显示肥大细胞含有丰富的分泌颗粒，它说明肥大细胞具备了为人体I型变态反应提供快速反应的物质基础。

Using immunofluorescence staining with antibodies of specific enzyme of mast celland phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning confocal microscope.

利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

Using immunofluorescence staining with antibodies of specific enzyme of mast cell and phalloidin. MC subset was defined by sorting and measuring of flow cytomery. Distribution of secretory vacuole and microfilament in MC were showed by laser scanning cofoncal microscope.

分泌方法：利用肥大细胞的特征性蛋白酶抗体和鬼比环肽的免疫荧光标记；以流式细胞仪检测分选人皮肤组织中肥大细胞的亚型；使用激光扫描共聚焦显微镜显示肥大细胞内分泌颗粒和微丝的分布。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。