肥大

It is one of the most important pathological foundations resulting in cardiogenic death.

背景与目的：心肌肥大是多种心血管疾病的共同病理过程，是导致心原性死亡的最重要的病理基础之一。

A 30-year-old female patient presented with macrodactyly of the left thumb, hemihypertrophy of the left lower extremity, plantar cerebriform hyperplastic mass and nodules for thirty years.

因左拇趾粗大、左下肢偏侧肥大、左足跖脑回状增生性斑块和结节30年就诊。

RESULTS: Twelve days after mandibular vertical functional positioning the condylar height increased. Nine days after mandibular vertical functional positioning the thickness of prechondroblast layer and chondroblast layer increased significantly compared with control groups. However the thickness of hypertrophic layer decreased significantly after 6 days' mandibular repositioning. No change was found in the thickness of mesenchymal layer during the experimental period.

结果：下颌垂直向功能性移位后12d，髁突高度较对照组显著增加，前斜面更倾斜；髁突后上区前成软骨细胞及成软骨细胞层厚度在实验第3~6天无明显差异，第9天开始出现显著变化，实验组较对照组显著增厚，这种变化持续到实验第12天；肥大软骨细胞层厚度在实验第3、9、12天，实验组与对照组无显著差异，在实验第6天较对照组显著减少，间充质细胞层变化不大。

Part one Isolating, culturing, chondroblast induction and evaluation of human MSC in vitroObjective:To examine the property and proliferation ability of primary culturing MSC ,mvestgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

结果：原代骨髓基质干细胞易于体外培养，增殖旺盛；经轻度离心和化学限定培养基诱导后细胞呈高密度生长，仍保持较旺盛的增殖能力，细胞转化成圆形肥大细胞，甲苯胺蓝染色显示软骨细胞外基质 v的特异性异染，11胶原蛋白免疫细胞化学染色呈强阳性，BT干CR鉴定显示＊胶原mRNA丰富的表达。

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的：在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段，并制备其鼠源性多克隆抗体。

Immunohistochemical stains were used to show the tryptase + mast cell and the chymase + mast cell distribution in order to observe the change of the mast cell in the model group, the control group and the treatment group.

取右下肺组织，用抗类胰蛋白酶单克隆抗体和抗类糜蛋白酶单克隆抗体免疫组织化学染色观察实验组与正常组之间肥大细胞数量的变化，HE染色观察肺组织一般病理变化。

The chymase levels were ditermined by using the method of enzyme-linked immunosorbant assay in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically by using anti-chymase antibody.

采用酶联免疫吸附实验检测45例慢性肝炎肝组织中Chymase浓度，观察不同的纤维化分期(S1-S4)，其肝组织中Chymase浓度；用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。

Results:Three immunophenotypes were determined: MCs positive for tryptase, no immunodetectable chymase; MCs positive for chymase, no immunodetectable tryptase ; and MCs positive for tryptase and chymase .

结果：三种免疫表型被确定：肥大细胞的类胰蛋白酶阳性、类糜蛋白酶阴性；类糜蛋白酶阳性、类胰蛋白酶阴性和类胰蛋白酶阳性、类糜蛋白酶阳性。

Using the SLC-2000B colposcope that was maded in the DIGENE company to examine and make biossy in the doubtful sites.

100例妇检发现宫颈肥大，并存在不同程度的糜烂，疑有宫颈癌变的患者，采用DIGENE公司生产的SLC-2000B阴道镜检查，并在检查出的可疑部位做组织活检。

In TUNEL assay it can be observed that cystamine can reduce myocardial cell apoptosis . At the same time Western Blot analysis to prove cystamine can reduce cardiac cell apoptosis and Deth receptor and Mitochondria through theses two pathsway, NF-κB protein expression significantly increased furthermore, it promots cell survival, which can reduce cardiac hypertrophy,apoptosis.

在TUNEL assay中可以观察到胱胺可以减少心肌细胞的凋亡，同时我们也利用Western Blot证明胱胺可以减少心肌细胞的凋亡是透过Deth receptor和Mitochondria这两条路径，而这样子降低凋亡的现象很有可能与NF-kB这讯息传递的分子上升有关，因此胱胺确实具有保护心肌细胞的作用，可改善心肌细胞肥大、凋亡的情形。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。