肠膜

To isolate, cultivate and identify the human embryonic fibroblasts derived from the gonadal ridges and dorsal mesenteries of human embryos, and to detect the expression by hEFs of cytokines crucial for the growth of human embryonic germ cells in vitro.

目的：从人胚胎生殖腺嵴和肠背系膜中分离、培养及鉴定人胚胎成纤维细胞，检测hEFs表达人胚胎生殖细胞体外生长所需的重要细胞因子。

The hEFs derived from gonadal ridges and dorsal mesenteries of human embryos are successfully isolated and cultivated,and the cells express the cytokines essential for the growth of hEG in vitro.

成功地分离和培养了人胚胎生殖腺嵴和肠背系膜来源的成纤维细胞，证明其表达对于hEG体外生长起重要作用的细胞因子。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

Methods The hEFs were isolated by enzyme digestion from gonadal ridges and dorsal mesenteries of 5- to 9- week old human embryos. The cells were then cultivated. The biologic characteristics (morphology, growth characteristics and cell cycle) of these cells were also studied.

利用酶消化法从孕5～9周龄人胚胎的生殖腺嵴和肠背系膜中分离培养hEFs，检测其生物学特性（细胞形态、细胞生长特点、细胞周期）。

Methods The hEFs were isolated by enzyme digestion from gonadal ridges and dorsal mesenteries of 5-to 9-week old human embryos.

利用酶消化法从孕5~9周龄人胚胎的生殖腺嵴和肠背系膜中分离培养hEFs，检测其生物学特性(细胞形态、细胞生长特点、细胞周期)。

In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at 21st day before birth. With the development of rat digestive tract, neurokinin A-immunoreactivity positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexes, while mature nerve fibres could be seen at one month after birth. 2. In rat stomach, the positive reaction of NKA initially happened in the myenteric plexus at the 14th day of embryo, and then appeared on circular muscles, longitudinal muscles, submucosal, muscularis mucosae, lamina propria and epithelium. 30 days after birth, expression of NKA is same as seen in adaulthood. 3. In rat small intestinal, the NKA-IR could first be found in the myenteric plexus of the duodenum, jejunum and ileum at 14th , 15th and 17th day before birth respectively. Then the NKA-IR occurred in the longitudinal muscle layer, circular muscle layer, intestinal villus, intestinal gland, muscularis mucosae, submucosa, submucosal plexes, mucosa plexus and deep muscular plexus.

结果 1、在食管，于胚胎21天的粘膜肌层和环肌层内观察到神经激肽A免疫阳性膨体纤维，出生后，随幼鼠的生长发育，相继在上皮，粘膜下层，纵肌层、肌间丛、粘膜下丛有NKA-IR表达，30天时已和成年鼠相似；2、在胃，首先于胚胎14天的肌间丛出现NKA-IR的表达，随发育相继在环肌、纵肌，粘膜下层、粘膜肌、固有膜及上皮内出现NKA-IR的表达，30天时具备成年鼠的分布特征；3、在小肠，分别于胚胎14、15、17天的十二指肠、空肠和回肠的肌间丛处出现NKA-IR的表达，随发育相继出现在纵肌、环肌、绒毛、小肠腺周、粘膜肌、粘膜下层、粘膜下丛、粘膜丛、深肌丛。

The embryonic development of Cynoglossus semilaevis was observed with tissue slice method. Notochord, optic vesicle, mesoderm, spinal cord floor-plate, neural tube, gut, otic vesicle, brain, oropharyngeal membrane and tubular heart of C.

对半滑舌鳎 Cynoglossus semilaevis 胚胎发育进行了组织学观察，首次描述了半滑舌鳎胚胎发育过程中脊索、眼囊、中胚层、脊髓底板、神经管、肠、耳囊、脑、口咽膜和心管等组织结构。

The embryonic development of Cynoglossus semilaevis was observed with tissue slice method. Notochord, optic vesicle, mesoderm, spinal cord floor-plate, neural tube, gut, otic vesicle, brain, oropharyngeal membrane and tubular heart of C.

中文摘要：对半滑舌鳎 Cynoglossus semilaevis 胚胎发育进行了组织学观察，首次描述了半滑舌鳎胚胎发育过程中脊索、眼囊、中胚层、脊髓底板、神经管、肠、耳囊、脑、口咽膜和心管等组织结构。

Objective To report the results of reversed sural neurovascular fascio cutaneous island flap in clinical application.

作者：匡亚华，董忠根编辑：studa20 目的报告带腓肠神经营养血管蒂逆行岛状筋膜皮瓣的临床运用效果。

PL could maintain cellular membrane integrity,induce the oxidative damage to biomembrane by free radicle and improve antioxygen ability and ATPase activity of larval fish.5 Effect of phospholipid supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvaePurpose:This study was undertaken to determine the effect of commercial PL supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvae,which can further investigated the mechanism of the growth-promoting effect of PL with the study of histology and digestive physicalogy.

磷脂能够维持黄颡鱼仔稚鱼细胞膜的完整性，降低自由基对生物膜的过氧化损伤，提高机体抗氧化系统的活力和ATP酶活性。5饲料中不同磷脂添加水平对黄颡鱼仔稚鱼前肠和肝脏组织学的影响目的：研究在实际配方的人工微粒饲料中添加商品大豆磷脂对黄颡鱼仔稚鱼消化肠道肝脏器官发育及脂肪消化吸收的影响，进一步从组织学消化生理角度揭示磷脂对仔稚鱼促生长作用机理。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。