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The virions were observed in the cylindrical cell at 9 h p.i.. The intact virions probably penetrated through the peritrophic membrane,then absorbed and invaded the microvilli for infecting the cylindrical cells.

作者认为病毒入侵是以整个病毒粒子穿越中肠围食膜,吸附并进入微绒毛,感染圆筒形细胞,与前人病毒入侵只是髓核进入细胞,而病毒衣壳仍留在细胞外的推测不同。

The biggest skin defect was 14 cm x 20 cm in size. Skin defects were repaired with flaps, myocutaneous flaps, free skin grafts and suturations. There were 4 gastronomies flaps, 6 latissimus flaps, 2 tensor fascia lata myocutaneous flaps, 1 transverse recuts abdominis myocutaneous flap, 5 plantaris medialis flaps, 3 random flaps, 12 free skin grafts and 5 suturings.

采用 7种不同类型的皮瓣、肌皮瓣、游离植皮和手术后直接缝合等方法进行修复,其中腓肠肌皮瓣 4例,背阔肌皮瓣6例,阔筋膜张肌皮瓣 2例,腹直肌皮瓣1例,足底内侧皮瓣 5例,邻近皮瓣3例,游离大块植皮1 2例,肿瘤切除后直接缝合5例。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果:从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs,该细胞可传25代以上,且经过传代及冻存复苏后生物学特性无改变;hEFs表达脯氨酰4-羟化酶β亚单位,不表达细胞角蛋白4,确定其为成纤维细胞;该成纤维细胞表达bFGF和LIF。

Objective To construct an expression vector for fusion gene of heat-liable enterotoxin B subunit and major outer membrane protein of Chlamydia psittaci and express the fusion gene in prokaryotic cells.

目的构建含大肠杆菌热不稳定肠毒素B亚单位和鹦鹉热衣原体主要外膜蛋白融合基因的表达载体,并在原核细胞中表达融合蛋白。

Chlamydia psittaci ; Escherichia coli heat-liable enterotoxin B subunit ; Major outer membrane protein ; Prokaryotic expression

鹦鹉热衣原体;大肠杆菌热不稳定肠毒素B亚单位;主要外膜蛋白;原核表达

The authors explain that in a standard blow-hole colostomy, the omentum and seromuscular layers of the colon are sutured to the peritoneum and the rectus fascia.

作者认为在标准的低位肠造瘘术中,结肠的浆膜和浆肌层与壁腹膜和腹横筋膜缝合在一起。

Objective To evaluate clinical effects of sural neurovascular fasciocutaneous flap on sensational reconstruction and repair of soft-tissue defects in foot.

目的 研究应用结扎小隐静脉的腓肠神经营养血管筋膜皮瓣修复足部软组织缺损,并重建皮瓣感觉功能的临床效果。

To study application sural neurovascular flap to repair tissue defect of down shank.

目的 研究应用逆行腓肠神经营养血管筋膜皮瓣修复小腿下段及足部组织缺损。

Methods: During intravenous anesthesia, twenty male SD rats received single transcranical magnetic and electric stimulation with Mag 2 stimulator and Cantada electromyograph. The characteristics of MEP recorded epidurally or in the bilateral forelimbs′ extensor muscles and hindlimbs′ gastrocnemius muscles under different stimulation intensities were observed.

20只雄性SD大鼠经静脉麻醉后采用 Mag 2型磁刺激仪和Cantada型肌电图仪分别进行单次经颅磁及电刺激,观察不同刺激强度下,在T12硬膜外和双侧前肢伸肌和后肢腓肠肌记录的MEP变化特征。

The basolateral M cell surface is invaginated to form an intracellular central pocket into and out of which lymphocytes and macrophages.

M细胞是一种特化的上皮细胞,多分布于Peyer's结圆顶区的周边,FAE的顶端未见M细胞;其结构有别于相邻的肠上皮细胞,表面没有长而整齐的微绒毛,取而代之的是粗而短的微皱褶,胞质内终末网不发达,溶酶体较少,基底膜向顶部穹隆状突起,形成一&口袋&样结构,其中含有多个免疫活性细胞。

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