肠肽酶

At early stage after brain injury, there were a quantity of fibrous callus and cartilaginous callus formation in brain injury and fracture group and many neuropeptides immunoreactive nerve fibers in callus were found. Strong immunoreactivites of CGRP, SP, VIP, NPY, TOH occurred to osteogenitor cells and chondroblast, which proliferated in thickened endothecium.

脑损伤合并骨折组早期形成大量纤维骨痂和软骨骨痂，骨痂中神经肽免疫阳性神经纤维较多，明显增厚的骨膜内层骨祖细胞、幼稚的软骨细胞胞质内降钙素基因相关肽、P物质、血管活性肠肽、酪氨酸羟化酶、神经肽Y强阳性表达。

Enterokinase was a serine protein hydroltyic enzyme that could recognize amino acid sequence DDDDK and cleave the peptide bond after "K".

肠激酶(enterokinase， EK)是一种专一识别DDDDK氨基酸序列、并水解K后肽键的丝氨酸蛋白水解酶。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Expression of PS2 and ITF was determined with RT-PCR,and the determination of contents of aninohexose and phosphatide were carried out.

逆转录-多聚酶链反应检测乳癌相关肽（PS2）、肠三叶因子基因表达变化，并同时检测氨基己糖及磷脂含量。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

Assulta and the induction of GST activity by nicotine and capsicine in H. assulta provided partial supports for the hypothesis that diet breadth was governed by detoxification abilities. The results also indicated that two sibling species had different strategies of adaptation to toxic substances: H. assulta saves expenditure of nutrients and energy by restricting their countermeasures to a few potentially toxic compounds. H. armigera, by contrast, must be prepared to counter a wider range of potential toxins. 5. Compared with parental species, hybrids of reciprocal hybridization had significant difference in feeding preference for hot pepper.

结果显示，广食性的棉铃虫在中肠羧酸酯酶和谷胱甘肽-S-转移酶活性方面高于寡食性的烟青虫，但烟青虫谷胱甘肽-S-转移酶活性可以被寄主植物内的烟碱和辣椒素所诱导，表明解毒酶活性在一定程度上影响着植食性昆虫的食性广度；同时也暗示着二近缘种对有毒的次生物质采取不同的适应策略：烟青虫有选择地对付种类较少的植物次生物质以减轻营养和能量的消耗；棉铃虫则必须对付存在于其寄主植物内的种类繁多的有毒次生物质。

PACAP is a pleiotropic neuropeptide that belongs to the secretin/glucagon/vasoactive intestinal peptide family.

垂体腺苷酸环化酶激活肽是多效性的神经肽，属于肠促胰液素/胰高血糖素/肠血管活性肽家族。

Abjective To study the expression of glutathione- S-transfereesand p53 in colorectal proliferate diseases and cancer and relationship.

目的 研究胎盘型谷胱甘肽转移酶与p53蛋白在肠腺癌和增生性病变中表达的意义。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

A total of 200 strains (z50% gentamicinresistant) from the United States were used for the cross-resistance study including Staphylococcus aureus (110), coagulase-negative staphylococci (CoNS; 50), Pseudomonas aeruginosa (10), Escherichia coli (20), and other Enterobacteriaceae (10) tested against TAO, bacitracin, polymyxin B, neomycin, amikacin, gentamicin, streptomycin, tobramycin, and mupirocin. Fifty gentamicin-resistant isolates from each year (1997–2002) were used to determine the activity of TAO over time. Baseline resistance rates of TAO among 300 Australian isolates (AGARS Program, 2002–2003) were also studied.

来至美国的200株菌株（ z50 ％对庆大霉素有耐药性）用于交叉耐药性研究，其中包括金黄色葡萄球菌（ 110株），凝固酶阴性葡萄球菌（ CoNS ； 50株），铜绿假单胞菌（ 10株），大肠埃希氏菌（ 20 株）和其他肠杆菌属菌（ 10株），检测这些菌对于以下抗生素的耐药性，抗生素为TAO，杆菌肽，多粘菌素B ，新霉素，丁胺卡那霉素，庆大霉素，链霉素，妥布霉素，莫匹罗星。1997到2002年期间每年分离出五十株对庆大霉素有抗药性菌株来确定随时间的变化TAO对其的活性是否有所改变。300株澳大利亚分离的菌株(AGARS Program， 2002–2003)也对TAO的耐药性基准进行了研究。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。