肠肥大

Mast cells could develop a key role in the expression of these three phenotypes because their mediators have the ability to produce all the aforementioned alterations, both at the splanchnic level (portal hypertensive enteropathy, mesenteric adenitis, liver steatosis) and the systemic level (portal hypertensive encephalopathy).

肥大细胞可能在这一过程中其关键作用，因为它的介质可以引起前面提到的变化，包括内脏（门静脉高压性肠病，肠系膜淋巴结炎，肝脂肪变性）和全身。

Objective:To investigate changes in activities of the intestinal mucosal mast cells in multiple organ failure.

目的 ：研究多器官衰竭时肠黏膜肥大细胞活性的变化，从黏膜免疫的角度探讨 MOF的病理生理机制。

Our results showed that LMWH at the concentrations of 16000and 8000 u·L(-1) could significantly (P.01, P.05 vs vehicle) inhibit constriction of isolated ileumsmooth muscle of ovalbumin-sensitized guinea pigs induced by OA in vitro, LMWH 200, 50and 12.5 u·kg(-1) ip could markedly (P.01 vs vehicle) inhibit degranulation of Pericranium MC inrats, LMWH 60 and 30 u·kg(-1) ip could significantly (P.01, P.05 vs vehicle) depress the lickingresponse induced by 4-aminopridine and the increasing of capillary permeability induced by aceticacid in mice.

研究结果显示，LMWH16000u·L(-1)（P＜0.01）和8000u·L(-1)（P＜0.05）均能显著降低OA所致的致敏豚鼠离体回肠平滑肌收缩高度；LMWH200u·kg(-1)，50u·kg(-1)和12.5u·kg(-1)（P＜0.01）均能明显抑制颅骨骨膜肥大细胞脱颗粒；LMWH60u·kg(-1)（P＜0.01）和30u·kg（P＜0.05）均能显著减少个氨基毗陡诱发的小鼠舔体反应次数，并能抑制冰乙酸所致的小鼠毛细血管通透性增加。

RESULTS:LMWH 16000 and 8000 u.L-1 could significantly inhibit the constriction of the isolated ileum smooth muscle of ovalbumin-sensitized guinea pigs induced by OA in vitro (P0.01,P0.05 vs vehicle);LMWH 200,50 and 12.5 u.kg-1ip could markedly inhibit the degranulation of pericranium MC in rats (P0.01 vs vehicle),LMWH 60 and 30 u.kg-1 ip could sigificantly depress the licking response induced by 4-aminopyridine and the increasing of capillary permeabili ty induced by acetic acid in mice (P0.01,P0.05 vs vehicle).

结果：LMWH 16000 u.L-1(P0.01)和8000 u.L -1(P0.05)均能显著降低OA所致的致敏豚鼠离体回肠平滑肌收缩高度；LMWH 200，50和12.5 u.kg-1 (P0.01)均能明显抑制颅骨骨膜肥大细胞脱颗粒；LMWH 60 u.kg-1(P0.01)和30 u.kg-1(P0.05)均能显著减少4-氨基吡啶诱发的小鼠舔体反应次数，并能抑制冰醋酸所致的小鼠毛细血管通透性增加。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin，OA)免疫豚鼠造成过敏模型，观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响；在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏，观察LMWH对OA引起肥大细胞脱颗粒的影响；以4-氨基吡啶诱发小鼠舔体反应，观察LMWH对舔体反应的影响；以冰醋酸使小鼠毛细血管通透性增加，观察LMWH对通透性的影响。

The correlation between ICJ mucosal MC counts and the number of SP positive fibers was found ( r =0.646 7, P .01), and MCs were close to SP positive fibers in lamina propria, which were demonstrated as unmyelinated nerves.

广泛分布于肠道黏膜和黏膜下层的肥大细胞(mastcell，MC)很有可能是这一机制中的一个中心环节，因为MC参与肠黏膜的免疫调节，受炎症刺激激化后，可分泌多种介质，如组胺、5 羟色胺、细胞介素

NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.

结果 丰富的NGFR染色阳性神经纤维分布于正常结肠环肌层及粘膜下层，少量分布于纵肌层，NGFR染色阳性神经元分布于肌间神经丛及粘膜下神经丛；NGFR 染色阳性神经纤维在HD无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如，而肌间肥大的神经干及粘膜下神经干为NGFR染色阳性，并且在其外周可见一强阳性染色带。

Histological observation showed that degeneration,necrosis of liver cells,edema,extravasated blood,erosion and degranulation of the mast cells in intestine mucosa.

肠谷氨酰胺酶活性降低，肠粘膜对谷氨酰胺利用减少，肠粘膜通透性增加。组织细胞学显示，TAA组动物肝细胞发生严重变性坏死；肠粘膜淤血、水肿、糜烂，肠肥大细胞脱颗粒。

Irritable bowel syndrome ; Mast cell ; Substance P ; Vasoactive intestinal ploypept ide ; Hypersensitivity

肠易激综合征；肥大细胞； P物质；血管活性肠肽；内脏高敏感性

Diseased cobias were collected from some net-cage farms at Hsiqu Liouchiou Island, next to Dunggang, Taiwan. Some little nodes appeared on surfaces of their fins and skins. When the symptom became serious, they looked like sarcomas. Almost every organ, including liver, spleen, kidney, fin, heart, stomach, intestine of a hard-sick cobia were dealt through the following processes:fixation, section and staining. Only at the connective tissues of fins and epithelium did we find hypertrophy cells which own big nuclei and inclusion bodies around the nuclei.

中文摘要本实验自屏东小琉球采集某家养殖业者箱网网中的罹病海鲡，外观上，鳍与部分体表出现结节状之小颗粒，严重时，呈现瘤状肉柄，取其各部位组织包括：肝脏、脾脏、肾脏、鳍、心脏、胃、肠等进行固定、切片及染色，发现唯有於鳍与皮肤部位之结缔组织细胞，明显可见肥大之现象，肥大之细胞拥有巨大细胞核与围绕著细胞核的包涵体网状结构，由於肥大细胞之堆叠，导致鱼体之鳍与一些表皮部位有颗粒状的「结节」存在。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。